Abstract
1. Ethyl methanesulphonate (BMS)-treated barley seeds stored at 30 % water content for 1 – 4 weeks are capable of recovering from the mutagen induced injury. The level and speed of the recovery depends on the EMS dose and storage period. Seeds treated with extremely severe EMS doses reducing the% of germination to 5 – 10 % cannot recover from the induced injury. 2. Due to a 1 to 6 weeks storage of EMS-treated seeds with 20 % water content the amount of induced injury increases in dependence on the applied EMS dose and storage period. Doses of EMS, reducing the seedling height in the M1 generation to 90 – 95 % of the control reveal no storage after-effects. 3. Storage of X-ray exposed seeds at 20 % or 30 % water content did not result in any significant changes in the yield of induced genetic damage.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Corry, P. M., Cole, A.: Double strana rejoining in mammalian DNA. - Nature New Biol.245: 100–101, 1973.
Curtis, H. J., Delihas, N., Caldbcott, R. S., Konzak, C. F.: Modifioation of radiation damage in dormant seeds by storage. - Radiat. Res.8: 526–534, 1958.
Ehrenberg, L.: Radiobiological mechanisms of genetic effects. A review of some current lines of research. - Radiat. Res. (Suppl.)1: 102–123, 1959.
FoUsová, S., Velemínský, J., Gichneb, T., Pokorný, V.: DNA synthesis during the early stage of germination in the barley embryo meristems and its inhibition by N-methyl-N-nitroso-urea. - Biol. Plant.16: 168–173, 1974.
Froese-Gertzen, E. E., Konzak, C. F., Nilan, R. A., Heiner, R. E.: The effeot of ethyl metha-nesulfonate on the growth response, chromosome structure and mutation rate in barley. - Radiat. Bot.4: 61–69, 1964.
Gaul, H.: Mutations in plant breeding. - Radiat. Bot.4: 155–232, 1964.
Gichner, T., Eheenberg, L.: The influence of post-treatment storage on the frequency of EMS-induced chromosomal aberration in barley. - Biol. Plant.8: 256–259, 1966.
Gichner, T., GaUl, H.: Storage effeot following treatment of barley seeds with ethyl methane-sulfonate, I. Influence of seed moisture content. - Radiat. Bot.11: 53–58, 1971.
Gichner, T., GaUL, H., OmuRa, T.: The influenoe of post-treatment washing and redrying of barley seeds on the mutagenic aotivity of N-methyl-N-nitroso-urea and N-ethyl-N-nitroso-urea. - Radiat. Bot.8: 499–507, 1968.
Gichner, T., Velemínský, J.: Differential response of mutagenic effects to storage of barley seeds treated with propyl methanesulphonate and isopropyl methanesulphonate. - Mutation Res.16: 35–40, 1972.
Howland, G. P., HaRt, R. W.: Radiation biology of cultured plant cells. - In:Reinert, J., Bajaj, Y. P. S. (ed.): Applied and Fundamental Aspacts of Plant Cell, Tissue, and Organ Culture. Pp. 731–754. Springer Verlag, Berlin, Heidelberg, New York 1977.
Joshi, R. K., GaUR, B. K., Notani, N. K.: Recovery from gamma radiation injury in barley eeeds. -Radiat. Bot.9: 141–145, 1969.
Kilbey, B. J.: Comparative aspects of mutagenesis inDrosophila and eukaryotic mieroorganisms. - Hereditas82: 43–50, 1976.
KÜnzel, G.: Vergleich der mutagenan Wirkung von N-Nitroso-N-Methylharnstoff, Äthyl-methansulfonat und Röntgenstrahlen bei Gerste. - Die Kulturpflanze19: 101–150, 1972.
Mikablsen, A., Ahnstböm, G., Li, W. C: Genetic effects of alkylating agents in barley. Influence of post-storage, metebolic statě and pH of mutagen solution. - Hereditas59: 353–374, 1968.
Strauss, B., Coyle, M., Mc Mahon, M., Kato, K., Dolyniuk, M.: DNA synthesis repair and chromosome breaks in eucaryotic cell. - In:Beers, R. F.,Herriott, R. M.,Tilghman, R. C. (ed.): Molecular and Cellular Repair Processes. - Johns Hopkins Med. J. (Suppl.) 1: 111 to 124, 1971.
Švachulová, J., Gichner, T., Vexemínský, J.: Respiration in barley seeds treated with mutagenic methyl methanesulphonate and stored at different water oontents. - Biol. Plant.15: 140–143, 1973.
Velemínský, J., Gichner, T.: Recovery from mutagen-induced injury and DNA repair in barley. - In:Gaul, H. (ed.): Barley Geneties. III. Proc. 3-rd International Barley Genetics Symposium. Pp. 146–154. Garching 1976.
Velemínský, J., ZadRaźil, S., Gichner, T.: Repair of single-strand breaks in DNA and recovery of induced mutagenic effects during the storage of ethyl methanesulphonate-treated seeds. - Mutation Res.14: 259–261, 1972.
Velemínský, J., Zadraźil, S., Pokoený, V., Gichneb, T., Švachulová, J.: Storage effect in barley. Changes in the amount of DNA lesions induced by methyl and ethyl methanesulphonate. - Mutation Res.19: 73–81, 1973.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gichner, T., Velemínský, J., Švachulová, J. et al. Response of “storage” effects in barley seeds to the dose of ethyl methanesulphonate and X-ray treatment. Biol Plant 19, 284–291 (1977). https://doi.org/10.1007/BF02923130
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02923130