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Macroligandd-alanyl-d-alanine-dextran for vancomycin purification

Equilibrium binding study

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Abstract

Water soluble macroligands for vancomycin purification in affinity ultrafiltration have been prepared by coupling the ligandd-alanyl-d-alanine to dextran activated by tosyl chloride, carbonyldiimidazole, and chloroformate, respectively. Centrifugal ultrafiltration has been used to study the equilibrium binding of vancomycin for the macroligands. The affinity binding can be described as Langmuir type adsorption and is strongly affected by temperature. The binding between vancomycin and macroligand is an unusual endothermic process that binding capacity of macroligand increases with temperature. Vancomycin has also been successfully purified from fermentation liquor using the macroligand in a centrifugal ultrafiltration device.

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Authors and Affiliations

  1. Department of Chemical Engineering, National Taiwan Institute of Technology, Taipei, 10772, Taiwan, R.O.C.

    Cheng-Kang Lee, Li-Chih Pan & Yi-Hsu Ju

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  1. Cheng-Kang Lee
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  2. Li-Chih Pan
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  3. Yi-Hsu Ju
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Lee, CK., Pan, LC. & Ju, YH. Macroligandd-alanyl-d-alanine-dextran for vancomycin purification. Appl Biochem Biotechnol 44, 21–30 (1994). https://doi.org/10.1007/BF02921848

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  • DOI: https://doi.org/10.1007/BF02921848

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