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Effects of carbodiimide structure on the immobilization of enzymes

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Abstract

A series of water-soluble disubstituted carbodiimides of different structure was tested for enzyme immobilization. In the experiments, a polyacrylamide-type bead polymer possessing carboxylic functional groups was used as support. The enzymes immobilized were amino-acylase (N-acylamino acid amidohydrolase; EC 3.5.1.14), arginase (L-arginine amidinohydrolase; EC 3.5.3.1), cyclodextrin glycosyltransferase (α-l,4-glucan 4-glycosyltransferase, cyclizing; EC 3.2.1.19), glucoamylase (1,4-α-D-glucan glycohydrolase, EC 3.2.1.3), and carboxypeptidase B (peptidyl-L-lysine [L-arginine] hydrolase; EC 3.4.17.2). It was found that the degree of immobilization strongly depended on the structure of carbodiimide used.

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Authors and Affiliations

  1. Reanal Factory of Laboratory Chemicals, Hungary and Polytechnical University of Budapest, Budapest 70, PO Box 54, H-1521, Budapest, Hungary

    B. SzajÀNi, P. SÜdi, Gabriella KlamÀr, Zsuzsa M. JÀszay, I. PetnehÀzy & L. TŐke

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  1. B. SzajÀNi
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  2. P. SÜdi
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  3. Gabriella KlamÀr
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  4. Zsuzsa M. JÀszay
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  5. I. PetnehÀzy
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  6. L. TŐke
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SzajÀNi, B., SÜdi, P., KlamÀr, G. et al. Effects of carbodiimide structure on the immobilization of enzymes. Appl Biochem Biotechnol 30, 225–231 (1991). https://doi.org/10.1007/BF02921689

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  • DOI: https://doi.org/10.1007/BF02921689

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