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Secretion of thermostable β-glucosidase by an intergeneric bacterial hybrid betweencellulomonas and bacillus subtilis

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Abstract

The intergeneric protoplast fusion hybrid (Bs/C 005) betweenCellulomonas sp. andBacillus subtilisproduced extracellular aryl β-glucosidase that is otherwise intracellular in parentalCellulomonassp. This extracellular aryl β-glucosidase was active at relatively higher temperature (60°C) and lower pH (pH 5.0) conditions than that ofCellulomonas enzyme. It also exhibited increased thermostability and stability over wide range of pH. Cellobiase activity, distinctly different from aryl β-glucosidase detected in bothCellulomonassp. Bs/C 005, was only intracellular. Cellobiase from Bs/C 005, however, was more thermostable than that ofCellulomonassp.

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Authors and Affiliations

  1. NCIM, Division of Biochemical Sciences, National Chemical Laboratory, 411 008, Pune, India

    D. V. Gokhale

  2. Molecular Biology Laboratory, Department of Zoology, University of Poona, 411 007, Pune, India

    D. N. Deobagkar

Authors
  1. D. V. Gokhale
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  2. D. N. Deobagkar
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Gokhale, D.V., Deobagkar, D.N. Secretion of thermostable β-glucosidase by an intergeneric bacterial hybrid betweencellulomonas and bacillus subtilis . Appl Biochem Biotechnol 26, 207–215 (1990). https://doi.org/10.1007/BF02921502

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  • DOI: https://doi.org/10.1007/BF02921502

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