Abstract
The production of some extracellular enzymes is known to be negatively affected by readily metabolized nitrogen sources such as NH4+ although there is no consensus regarding the involved mechanisms. Asparaginase II is a periplasmic enzyme ofSaccharomyces cerevisiae encoded by theASP3 gene. The enzyme activity is not found in cells grown in either ammonia, glutamine, or glutamate, but it is found in cells that have been subjected to nitrogen starvation or have been grown on a poor source of nitrogen such as proline. In this report it is shown that the formation of this enzyme is dependent upon the functionalGLN3 gene and that the response to nitrogen availability is under the control of theURE2 gene product. In this respect the expression of ASP3 is similar to the system that regulates theGLN1, GDH2, GAP1, andPUT4 genes that codes for glutamine synthetase, NAD-linked glutamate dehydrogenase, general amino-acid permease, and high affinity proline permease, respectively.
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References
Cohen, B. L. (1972),J. Gen. Microbiol. 71, 293–299.
Cohen, B. L. and Drucker, H. (1977),Archives Biochem. Biophys. 182, 601–613.
Keyser, P., Kirk, T. K., and Zeikus, J. G. (1978),J. Baderiol. 135, 790–797.
Kirk, T. K. and Fenn, P. (1981),Arch. Microbiol. 130, 59–65.
Fenn, P., Choi, S., and Kirk T. K. (1981),Arch. Microbiol. 130, 66–71.
Reid, I. D. (1983),Appl. Environ. Microbiol. 45, 830–837.
Reid, I. D. (1983),Appl. Environ. Microbiol. 45, 838–842.
Commanday, F. and Macy, J. M. (1985),Arch. Microbiol. 142, 61–65.
Magasanik, B. (1992), inThe Molecular and Cellular Biology of the Yeast Saccharomyces:Gene Expression, Vol.2, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 283–317.
Dunlop, P. C. and Ronn, R. J. (1975),J. Bacterial 122, 1017–1024.
Wiame J. M., Grenson M., and Arst H. N. (1985),Adv. Microb. Physiol. 26, 1–87.
Kim, K. W., Kamerud, J. Q., Livingston, D. M., and Roon, R. J. (1988),J. Biol Chem. 263, 11, 948-11,953.
Dunlop, P. C, Meyer, G. M., and Roon, R. J. (1980),J. Barteriol. 143, 422–126.
Kamerud, J. Q. and Roon, R. J. (1986),J. Bacteriol. 165, 293–296.
Coschigano, P. W. and Magasanik, B. (1991),Mol. Cell. Biol. 11, 822–832.
Miller, S. M. and Magasanik, B. (1991),Mol. Cell. Biol. 11, 6229–6247.
Minehart, P. L. and Magasanik, B. (1991),Mol. Cell. Biol. 11, 6216–6228.
Minehart, P. L. and Magasanik, B. (1992),J. Bacteriol. 174, 1828–1836.
Daugherty, J. R., Rai, R., ElBerry, J. M., and Cooper, T. G. (1993),J. Bacteriol. 175, 64–73.
Kim, K. W. and Roon, R. J. (1984),J. Bacteriol. 157, 958–961.
Ito, H., Fukada, Y., Murato, K., and Kimiru, A. (1983),J. Bacteriol. 153, 163–168.
Sambroock, J., Fritsch, E. F., and Maniatis, T. (1989),Molecular Cloning. A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Plainview, NY.
Sherman, F., Fink, G. R., and Lawrence, C. W. (1978),Methods in Yeast Genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
Pauling, K. D. and Jones, G. E. (1980),Biochim. Biophys. Acta 616, 271–282.
Pauling, K. D. and Jones, G. E. (1980),J. Gen. Microbiol. 117, 423–130.
Jones, G. E. (1977),J. Bacteriol. 130, 128–130.
Courchesne, W. E. and Magasanik, B. (1988),J. Bacteriol. 170, 708–713.
Fu, Y. and Marzluf, G. A. (1990),Proc. Natl. Acad. Sci. USA 87, 5331–5335.
Kudla, B., Caddick, M. X., Langdon, T., Martinez-Rossi, N., Bennet, C. F., Silbley, S., Davies, R. W. and Arst, H. N. (1990),EMBO J. 9, 1355–1364.
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Bon, E.P.S., Carvajal, E., Stanbrough, M. et al. Asparaginase II ofSaccharomyces cerevisiae . Appl Biochem Biotechnol 63, 203–212 (1997). https://doi.org/10.1007/BF02920425
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DOI: https://doi.org/10.1007/BF02920425