Abstract
A microbubble dispersion (MBD) was used to supply oxygen for aerobic fermentations in a standard 2 L stirred tank fermenter. The microbubble dispersion was formed using only surfactants produced naturally. Growth rates ofSaccharomyces cerevisiae cultures were found to be equal or greater with MBD sparging than with gas sparging. The oxygen transfer coefficent with MBD sparging was found to be 190/h and independent of impeller speed from 100–580 rpm. The oxygen transfer coefficient with air sparging rose from 55 to 132/h over the same range of impeller speeds. Power requirements for the fermenter systems were estimated.
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Yoshida, F., Ikeda, A., Imakawa, S., and Miura, Y. (1960),Industrial and Engineering Chemistry 52, 435–438.
Wallis, D. A., Lavinder, S. R., Michelsen, D. L., and Sebba, F. (1986),1986 Summer National Meeting of the American Institute of Chemical Engineers, Boston, MA.
Sebba, F. (1971),J. Coll. Inter. Sci. 35, 643–646.
Seba, F. (1985),Chemistry and Industry, February 4, 91–92.
Michelsen, D. L., Foreman, W., and Michelsen, D. (1988),Separations Innovative Concepts Fair, US DOE (PNL), 9.1–9.19.
Michelsen, D. L., Habash, S. M., Violette, D. L., Brown, D. G., and Sebba, F. (1989),Emerging Technologies for Hazardous Waste Treatment, ACS, I&EC, Atlanta, GA.
Bradley, K. A. and Barnett, S. M. (1981),182nd Meeting of the American Chemical Society.
Misra, T. K. and Barnett, S. M. (1986),World Congress III of Chemical Engineering, Tokyo, Japan, 905–908.
Cooper, T. G. (1977),The Tools of Biochemistry, Wiley, New York, pp. 57–59.
Hug, H. and Fiechter, A. (1973),Archiv für Mikrobiologie 88, 77–86.
Cooper, C. M., Fernstrom, G. A., and Miller, S. A. (1944),Indus. Eng. Chem. 36, 504–509.
Mateles, R. I. (1971),Biotechnol. Bioeng. 13, 581–582.
Chemical Engineers’ Handbook, 5th ed., (1973), Perry, R. H. and Chilton, C. H., eds., McGraw-Hill, New York, pp. 19-6–19-8.
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Kaster, J.A., Michelsen, D.L. & Velander, W.H. Increased oxygen transfer in a yeast fermentation using a microbubble dispersion. Appl Biochem Biotechnol 24, 469–484 (1990). https://doi.org/10.1007/BF02920271
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DOI: https://doi.org/10.1007/BF02920271