Abstract
A process for the continuous production of high purityL-lactic acid in a membrane bioreactor at 65°C has been developed. Two differentBacillus stearothermophilus strains have been tested in batch experiments. Lactic acid yields are between 60 and more than 95% of theoretical yields. The amounts of ethanol, acetate, and formate formed varied between 0 and 0.4, 0 and 0.1, and 0 and 0.5, respectively (mol/mol glucose). All byproducts are valuable and may be separated easily by rectification of the fermentation broth. Complete cell retention enables high volumetric productivity (5 g/Lh), and a minimum of growth supplements. The high temperature of 65°C allows the autoselective fermentation without problems with contamination.
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References
Litchfield, J. H. (1996),Adv. Appl. Microbiol. 42, 45–96.
Lipinsky, E. S. and Sinclair, R. G. (1986).Chem. Eng. Prog. 82, 26.
Lerner, M., (1996),Chem Marketing Reporter May 13, 7–18.
McCoy, M. (1996),Chem Marketing Reporter. September 16, 1992.
Kharas, G. B., Sanchez-Riera, F., and Severson, D. K. (1994), inPlastic from microbes, Mobley D. P., ed., Hanser/Gardner, pp. 93–132.
Tyagi, R. D., Kluepfel, D., and Couillard, D. (1991), inBioconversion of Waste Materials to Industrial Products, Martin, A.M. (ed.), Elsevier, Essex, UK.
Sneath, P. H. A., Mair, N. S., Sharpe, M. E., and Holt, J. G. (1986),Bergey’s Manual of Systematic Bacteriology, Williams & Wilkins, Baltimore.
Ferras, E., Minier, M., and Goma, G. (1986),Biotechnol. Bioeng. 28, 523–533.
Taniguchi, M., Kotani, N., and Kobayashi, T. (1987),Appl. Microbiol. Biotechnol. 25, 438–441.
Blanc, P. and Goma, G. (1987),Bioprocess Eng. 2, 137–139.
Borgardts, P., Krischke, W., Chmiel, H., and Trosch, W. (1994), Proceedings ECB 6, inProgress in Biotechnology 9, 905–908. Elsevier.
Mehaia, M., and Cheryan, M. (1985),Enzyme Microb. Technol. 8, 289–292.
Jones, D., Pell, R., Sneath, R. H. A. (1991), inMaintenance of Microorganisms and Cultured Cells. A manual of Laboratory Methods. 2nd ed. Kirsop B. E. and Doyle A. Academic New York, 45–50.
Gawehn, K. (1984), inMethods of Enzymatic Analysis 3rd ed., vol., Bergmeyer, H. U., (ed.), Verlag Chemie, Weinheim, Deerfield Beach Florida, Basel, pp. 588–592.
Hartley, B. S., Baghaei-Yazdi, N., Javed, M., Jackson, R. A., San Martin, R., and Leak, D. J. (1993), Straw 93’ Conference, Royal Agricultural College, Cirencester, Gloucestershire, UK.
Boyaval, P., Corre, C, and Terre, S. (1987),Biotechnol. Lett 9/3, 207–212.
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Danner, H., Neureiter, M., Madzingaidzo, L. et al. Bacillus stearothermophilus for thermophilic production of l-lactic acid. Appl Biochem Biotechnol 70, 895–903 (1998). https://doi.org/10.1007/BF02920200
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DOI: https://doi.org/10.1007/BF02920200