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Production and purification of CGTase of alkalophylicBacillus isolated from Brazilian soil

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Abstract

Alkalophylic bacilli that produce cyclodextringlycosyltransferase (CGTase) were isolated from Brazilian soil, with a scheme of two plating steps. In the first step, the bacterial isolate forms a halo in the cultivation medium that contains γ-cyclodextrin (CD) complexing dyes. The CGTase of an isolate was purified 157-fold by biospecific affinity chromatography, with β-CD showing a mol wt of 77,580 Daltons. It produces a γ- to β-CD ratio of 0.156 and a small amount of α-CD, using maltodextrin 10% as substrate, at 50°C, pH 8.0 and 22 h reaction time, reaching 21.4% conversion of the substrate to cyclodextrins. In the second screening step, the isolates chosen give larger halos with β-CD complexing dyes, and smaller halos with β-CD complexing dyes, leading to a 30% improvement in γ-CD selectivity, although at lower total yield for cyclodextrins (11.5%).

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Authors and Affiliations

  1. Chemical Engineering Department, State University of Marin’a, Av. Colombo, 5790-Bl. E46-09, 87020-900, Maringá, PR, Brazil

    Graciette Matioli & Gisella M. Zanin

  2. Biochemistry Department, Federal University of Paraná, P. O. Box 19046, 81531-990, Curitiba-PR, Brazil

    Manoel F. Gljimarães & Flávio f. De moraes

Authors
  1. Graciette Matioli
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  2. Gisella M. Zanin
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  3. Manoel F. Gljimarães
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  4. Flávio f. De moraes
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Correspondence to Flávio f. De moraes.

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Matioli, G., Zanin, G.M., Gljimarães, M.F. et al. Production and purification of CGTase of alkalophylicBacillus isolated from Brazilian soil. Appl Biochem Biotechnol 70, 267–275 (1998). https://doi.org/10.1007/BF02920143

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