Summary
Heat-killedPropionibacterium acnes (P. acnes) was intravenously injected into tuberculin-sensitized guinea pigs, and 7 days later, purified protein derivative (PPD) was also injected intravenously resulting in marked reductions in bile flow and bile acid excretion. Serum levels of bile acids and cholesterol were increased, and the activities of alkaline phosphatase (ALK-p) and leucine aminopeptidase (LAP) were also elevated. Histological examination revealed dilatation of bile canaliculi and diminution of microvilli. These results indicated that intrahepatic cholestasis was induced. These pathological changes were not detected in guinea pigs in which PPD was not injected. In this experimental model, the tuberculin-sensitized lymphocytes were infiltrated into the liver byP. acnes injection and the cholestatic factor was produced by PPD injection, resulting in the induction of intrahepatic cholestasis.
Similar content being viewed by others
References
Mizoguchi Y, Ohnishi F, Monna T, et al: Studies on intrahepatic cholestasis in drug-induced allergic hepatitis: Intrahepatic cholestasis induced in rat by the culture supernatant of activated lymphocytes. Gastroenterology 1979;77:A28
Mizoguchi Y, Monna T, Yamamoto S, et al: Studies on drug-induced allergic intrahepatic cholestasis: Hepatic cholestasis induced in dogs by lymphocyte culture supernatant. Gastroenterol Jpn 1979, 14:19–23
Mizoguchi Y, Ohnishi F, Monna T, et al: Studies on intrahepatic cholestasis in drug-induced allergic hepatitis: Intrahepatic cholestasis induced in the rat by the culture supernatant of activated lymphocytes. Hepato-gastroenterol 1981;28;147–150
Mizoguchi Y, Sakagami Y, Monna T, et al: Participation of the cholestatic factor in the pathogenesis of intrahepatic cholestasis found in various liver diseases. Gastroenterol Jpn 1982; 17:224–229
Mizoguchi Y, Sakagami S, Sawai H, et al: Modulation of cholestatic factor production by serum components. Gastroenterol Jpn 1983;189:372–376
Mizoguchi Y, Ohnishi F, Monna T, et al. Studies on intrahepatic cholestasis: Intrahepatic cholestasis induced in the rat by the culture supernatant of activated lymphocytes. Hepatogastroenterol 1981;28:292–295
Sakagami Y, Miyajima K, Tsutsui H, et al: Studies on the action mechanism of cholestatic factor: Effects on the bile acid-dependent and independent bile flow and erythritol clearance. Jpn J Gastroenterol 1982; 79:2121–2126 (in Jpn)
Mizoguchi Y, Ohnishi F, Monna T, et al: Partial purification of the cholestatic factor derived from the lymphocytes of tuber-culin-sensitized guinea pigs. Gastroenterol Jpn 1981; 16:260–267
Marbet UA, Shefer S, Leevy CM: Studies of the influence of immunological and serological factors from patients with cholestasis due to alcoholic or viral hepatitis on biliary function in the rat. Eur J Clin Invest 1984; 14:346–353
Tsutsui H, Mizoguchi Y, Yamamoto S, et al: Studies on experimentally-induced acute hepatic failure: Possible involvement of activated liver adherent cells. In: Kirn A, Knook DL, Wisse E, eds. Cells of the hepatic sinusoid. vol 1. The Kupffer Cell Foundation, Amsterdam. 1986;307–314
Sandberg DH, Sjövall K, et al: Measurement of human serum bile acid by gas-liquid chromatography. J Lipid Res 1965;6:182–192
Malloy HT, Evelyn KA: The determination of bilirubin with photoelectric colorimeter. J Biol Chem 1937; 119:481–490
Kind PRN, King EJ: Estimation of plasma phosphatase by determination of hydrolyzed phenol with aminoantipyrine. J Clin Path 1954, 7:322–326
Goldberg JA, Rutenburg AM: The colorimetric determination of leucine aminopeptidase in urine and serum of normal subjects and patients with cancer and other diseases. Cancer 1958;11:283–291
Richmond W: Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum. Clin Chem 1973;19:1350–1356
Bergmeyer HU, Scheibe P, Wahlefeld AW: Optimization of methods for aspartate aminotransferase and alanine amino-transferase. Clin Chem 1978;24:58–73
Mizoguchi Y, Sakagami Y, Tsutsui H, et al: Participation of cholestatic factor in the pathogenesis of intrahepatic cholestasis in acute viral hepatitis. Jpn J Allergol 1983;32:193–198
Mizoguchi Y, Sakagami Y, Yamamoto S, et al: Possible participation of cholestatic factor in the pathogenesis of intrahepatic cholestasis in alcoholic liver disease. Jap J Med 1985;24:135–140
Mizoguchi Y, Miyajima K, Sakagami Y, et al: Detection of the cholestatic factor in the liver tissue of patients with acute intrahepatic cholestasis. Ann Allergy 1986;56:304–307
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mizoguchi, Y., Sakagami, Y., Miyajima, K. et al. Experimental immunological intrahepatic cholestasis model. Gastroenterol Jpn 23, 18–22 (1988). https://doi.org/10.1007/BF02918851
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02918851