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Viability of spores after repeate freezing and thawing shocks

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Abstract

Survival of spores of the fungusRhizopus nigricans after repeated freezing and thawing was investigated. The cooling rate was 104°C/min. Dry spores were fully inactive after 32 repeated shocks. About one-half of spores were killed after 8 repetitions. The water content did not change the resistance, swollen spores reacted to shocks much like dry ones. The sensitivity of spores to freezing-thawing shocks increased considerably when the spores changed from the dormant to the active state. Already after a 30 min cultivation of spores in the nutrient medium two freezing and thawings were sufficient for inactivation of 60% spores. After a 90 min cultivation one freezing and one thawing were sufficient to inactivate practically all spores.

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Authors and Affiliations

  1. Department of Biology, Faculty of Medicine, J. E. Purkyně University, 662 43, Brno

    O. Nečas & M. Gabriel

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  1. O. Nečas
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  2. M. Gabriel
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Nečas, O., Gabriel, M. Viability of spores after repeate freezing and thawing shocks. Folia Microbiol 23, 126–132 (1978). https://doi.org/10.1007/BF02915312

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  • DOI: https://doi.org/10.1007/BF02915312

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