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Improvement ofArabidopsis thaliana seed transformation efficiency

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Biologia Plantarum

Abstract

Agrobacterium tumefaciens induced transgenosis by treatment of germinatingArabidopsis thaliana seed embryos has been achieved with differentAgrobacterium strains including the strain LBA4404, which was ineffective in seed transformation experiments of the other authors. The frequency of transgenosis was increased several times by application of acetosyringone to the growingA. tumefaciens suspension cultures. The DNA demethylating agent 5-azacytidine partly restored the distorted Mendelian segregation ratios in the offspring of transgenic plants.

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Abbreviations

AS:

acetosyringone

5-AC:

5-azacytidine

Kmr :

kanamycin resistant

Km5 :

kanamycin sensitive

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Authors and Affiliations

  1. Institute of Plant Molecular Biology, Academy of Sciences of Czech Republic, Branišovská 31, CZ-370 05, České Budějovice, Czech Republic

    D. Pavingerová & M. Ondřej

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  1. D. Pavingerová
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  2. M. Ondřej
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Pavingerová, D., Ondřej, M. Improvement ofArabidopsis thaliana seed transformation efficiency. Biol Plant 37, 467–471 (1995). https://doi.org/10.1007/BF02914001

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  • DOI: https://doi.org/10.1007/BF02914001

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