Abstract
Cadmium (Cd) and kinetin (Kin) significantly affected the growth and contents of chlorophyll (Chl) and of soluble and reserve (hydrolysable) saccharides in sunflower plants. Cd-treated plants had lower contents of Chl and soluble saccharides and produced less dry matter than control (Cd-untreated) plants. Chla stability to heat (CSI) increased at all Cd concentrations. The same was true for Chlb stability (0–10 μM Cd). Spraying sunflower shoots with Kin solutions counteracted the deleterious effects of Cd. Kin application enhanced the Chla andb contents, Chla/b ratio, content of soluble saccharides and dry matter, and to less extent Chl stability. The relative role of Kin in affecting the parameters tested (as indicated by η2 values) was predominant while that of Cd was subsidiary except for Chla stability. The role of Cd×Kin interaction was dominant for hydrolysable saccharides. Hence spraying shoots of plants grown under increasing Cd concentration with Kin can partially alleviate inhibitory effects of cadmium.
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Banerji, D., Laloraya, M.M.: Chlorophyll formation in isolated pumpkin cotyledons in the presence of kinetin and chloramphenicol.—Plant Cell Physiol.8: 263–268, 1967.
Baszynski, T., Wajda, L., Krol, M., Wolinska, D., Krupa, Z., Tukendorf, A.: Photosynthetic activities of cadmium-treated tomato plants.—Physiol. Plant.48: 365–370, 1980.
Beevers, L.: Growth regulator control of senescence in leaf discs of nasturtium (Tropaeolum majus).— In: Weightman, F., Setterfield, G. (ed.): Biochemistry and Physiology of Plant Growth Substances. Pp. 1417–1435. Runge Press, Ottawa 1968.
Bishnoi, N.R., Sheoran, I.S., Singh, R.: Influence of cadmium and nickel on photosynthesis and water relations in wheat leaves of different insertion level.—Photosynthetica28: 473–479, 1993.
Czuba, M., Ormrod, D.: Effects of cadmium and zinc on ozone-induced phytotoxicity in cress and lettuce.—Can. J. Bot.52: 645–649, 1973.
Dubois, M., Gilles, K.A., Hamilton, J.K., Rabers, R.A., Smith, F.: Colorimetric method for the determination of saccharides and related substances.—Anal. Chem.28: 350–356, 1956.
Ernst, W.H.O.: Biochemical aspects of cadmium in plant.—In: Nriagu, J.O. (ed.): Cadmium and Environment. Part I: Ecological Cycling. Pp. 639–653. Wiley Interscience, New York 1980.
Ferretti, M., Ghisi, R., Merlo, L., dalla Vecchia, F., Fassera, C.: Effect of cadmium on photosynthesis and enzymes of photosynthetic sulphate and nitrate assimilation pathways in maize (Zea mays L.).—Photosynthetica29: 49–54, 1994.
Foy, C.D., Chaney, R.L., White, M.C.: The physiology of metal toxicity in plants.—Annu. Rev. Plant Physiol.29: 511–566, 1978.
Gadallah, M.A.A.: The combined effects of acidification stress and kinetin on chlorophyll content, dry matter accumulation and transpiration coefficient inSorghum bicolor plants.—Biol. Plant.36: 149–153, 1994.
Huang, C.Y., Bazzaz, F.A., Vanderhoef, L.N.: The inhibition of soybean metabolism by cadmium and lead.—Plant Physiol.54: 122–124, 1974.
Imai, I., Siegel, S.: A specific response to toxic cadmium levels in red kidney bean embryos.—Physiol. Plant.29: 118–120, 1973.
Keul, M., Rodica, A., Kazar Keul, G., Vintila, R.: Accumulation and effect of lead and cadmium in wheat and corn.—Stud. Cercet. Biol.31: 49–54, 1980.
Lagerwerff, J.V.: Lead, mercury and cadmium as environmental contaminants.—In: Mortvedt, J.J., Giardano, P.M., Lindsay, W.L. (ed.): Micronutrients in Agriculture. Pp. 619–628. Soil Sci. Soc. Amer., Madison 1972.
Meidner, H.: The effect of kinetin on stomatal opening and the rate of intake of carbon dioxide in mature primary leaves of barley.—J. exp. Bot.18: 556–561, 1967.
Murty, K.S., Majumder, S.K.: Modifications of technique for determination of chlorophyll stability index in relation to studies of drought resistance in rice.—Curr. Sci.31: 470–471, 1962.
Ostle, B.: Statistics in Research.—Iowa State Univ. Press, Ames 1963.
Påhlsson, A.M.B.: Toxicity of heavy metals (Zn, Cu, Cd, Pb) to vascular plants.—Water Air Soil Pollut.47: 287–319, 1989.
Pettersson, O.: Differences in cadmium uptake between plants species and cultivars.—Swed. J. agr. Res.7: 21–24, 1977.
Pucher, W.G., Leavens Worth, C.S., Vickery, H.B.: Determination of starch in plant tissues.—Anal. Chem.20: 850–853, 1948.
Sabater, B., Rodriquez, M.T.: Control of chlorophyll degradation in detached leaves of barley and oat through effect of kinetin on chlorophyllase levels.—Physiol. Plant.43: 274–276, 1978.
Salama, F.M., Awadalla, A.A.: The effect of different kinetin application methods on some chlorophyll parameters of two crop plants grown under salinity stress.—Phyton27: 181–193, 1987.
Shlyk, A.A., Averina, N.G.: [Nature of the influence of kinetin on the process of accumulation of protochlorophyllide in etiolated and green barley leaves.]—Dokl. Akad. Nauk SSSR213: 235–238, 1973. [In Russ.]
Stiborová, M., Ditrichová, M., Březinová, A.: Effect of heavy metal ions on growth and biochemical characteristics of photosynthesis of barley and maize seedlings.—Biol. Plant.29: 453–467, 1987.
Stobart, A.K., Shewry, P.R., Thomas, D.R.: The effect of kinetin on chlorophyll synthesis in ageing etiolated barley leaves exposed to light.—Phytochemistry11: 571–577, 1972.
Stobart, A.K., Griffiths, W.T., Ameen-Bukhari, I., Sherwood, R.P.: The effect of Cd2+ on the biosynthesis of chlorophyll in leaves of barley.—Physiol. Plant.63: 293–298, 1985.
Todd, G.W., Basler, E.: Fate of various protoplasmic constituents in droughted wheat plants.—Phyton22: 79–85, 1965.
Vallee, B.L., Ulmer, D.D.: Biochemical effects of mercury, cadmium and lead.—Annu. Rev. Biochem.41: 91–128, 1972.
Van Assche, F., Clijsters, H.: Effects of metals on enzyme activity in plants.—Plant Cell Environ.13: 195–206, 1990.
Varma, M.M., Katz, J.E.: Environmental impact of cadmium.—J. environ. Health40: 308–314, 1978.
Walker, W.R., Miller, J.E., Hassett, J.J.: Effect of lead and cadmium upon the calcium, magnesium, potassium and phosphorous concentration in young corn plants.—Soil Sci.124: 145–151, 1977.
Weigel, H.J.: Inhibition of photosynthetic reactions of isolated chloroplasts by cadmium.—J. Plant Physiol.119: 179–189, 1985.
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Gadallah, M.A.A. Effects of cadmium and kinetin on chlorophyll content, saccharides and dry matter accumulation in sunflower plants. Biol Plant 37, 233–240 (1995). https://doi.org/10.1007/BF02913219
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DOI: https://doi.org/10.1007/BF02913219