Skip to main content
Log in

Identification and relationships of cultivated accessions fromLolium-Festuca complex based on RAPD fingerprinting

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

Randomly Amplified Polymorphic DNA (RAPD) technique with 15 arbitrary primers was used to identify and reveal relationships of the accessions comprising 4 species ofLolium-Festuca complex. Altogether 252 RAPD markers were considered in statistical treatment, 60 of which could be identified as potentially taxon-specific. All analyzed taxa were fully distinguishable using RAPD markers.Lolium-Festuca relationships based on RAPD data were evaluated using cluster analysis (UPGMA) and principle coordinate analysis (PCO). The results of UPGMA as well as PCO performed on data pooled from all RAPD profiles support separation of the two generaLolium andFestuca.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Amasino, R.M., Powell, A.L.T., Gordon, M.P.: Changes in T-DNA methylation and expression are associated with phenotypic variation and plant regeneration in a crown gall tumor line.—Mol. gen. Genet.197: 437–446, 1984.

    Article  PubMed  CAS  Google Scholar 

  • Arnold, M.L., Buckner, C.M., Robinson, J.J.: Pollen-mediated introgression and hybrid speciation in Lousiana irises.—Proc. nat. Acad. Sci. USA88: 1398–1402, 1991.

    Article  PubMed  CAS  Google Scholar 

  • Bassam, B.J., Caetano-Anolles, G., Gresshoff, P.M.: Fast and sensitive silver staining of DNA in polyacrylamide gels.—Anal. Biochem.80: 81–84, 1991.

    Google Scholar 

  • Benson, D., Lipman, D.J., Ostell, J.: GenBank.—Nucl. Acids Res.21: 2963–2965, 1993

    Article  PubMed  CAS  Google Scholar 

  • Bulińska-Radomska, Z., Lester, R.N.: Relationships between five species ofLolium (Poaceae)— Plant Syst. Evol.148: 169–175, 1985a.

    Article  Google Scholar 

  • Bulińska-Radomska, Z., Lester, R.N.: Relationships between three species ofFestuca sect.bovinae (Poaceae) —Plant Syst. Evol.149: 135–140, 1985b.

    Article  Google Scholar 

  • Bulińska-Radomska, Z., Lester, R.N.: Intergeneric relationships ofLolium, Festuca, andVulpia (Poaceae) and their phylogeny.—Plant. Syst. Evol.159: 217–227, 1988.

    Article  Google Scholar 

  • Caetano-Anolles, G., Bassam, B.J., Gresshof, P.M.: DNA amplification fingerprinting using very short arbitrary oligonucleotide primers.—Biotechnology9: 553–557, 1991.

    Article  PubMed  CAS  Google Scholar 

  • Charmet, G., Balfourier, F., Ravel, C.: Isozyme polymorphism and geographic differentiation in a collection of French perennial ryegrass populations.—Genet. Resour. Crop. Evolut.40: 77–89, 1993.

    Article  Google Scholar 

  • Crawford, D.J.: Electrophoretic data and plant speciation.—Syst. Bot.10: 405–416, 1985.

    Article  Google Scholar 

  • Griffith, S.M., Banowetz, G.M.: Differentiation ofLolium perenne L. andL. multiflorum Lam. seeds by two isoesterase isoforms.—Seed Sci. Technol.20: 343–348, 1992.

    Google Scholar 

  • Darbyshire, S.J., Warwick, S.I.: Phylogeny of North AmericanFestuca (Poaceae) and related genera using chloroplast DNA restriction site variation.—Can. J. Bot.70: 2415–2429, 1992.

    Article  Google Scholar 

  • Darbyshire, S.J.: Realignment ofFestuca subgenusSchedonorus with the genusLolium (Poaceae)— Novon3: 239–243, 1993.

    Article  Google Scholar 

  • Dinelli, G., Bonetti, A.: Capillary electrophoresis in species and cultivar determination.—Seed Sci. Technol.20: 561–569, 1992.

    Google Scholar 

  • Griffith, S.M., Banowetz, G.M.: Differentiation ofLolium perenne L. andL. multiflorum Lam. seeds by two isoesterase isoform.—Seed Sci. Technol.20: 343–348, 1992.

    Google Scholar 

  • Hayward, M.D., Olesen, A., Due, I.K., Jenkins, R., Morris, P.: Segregation of isozyme marker loci amongst androgenetic plants ofLolium perenne L..—Plant Breed.104: 68–71, 1990.

    Article  Google Scholar 

  • Livesey, V., Norrington-Davies, J.: Isozyme polymorphism inFestuca rubra L..—Euphytica55: 73–79, 1991.

    Article  CAS  Google Scholar 

  • Moller, M., Spoor, W.: Discrimination and identification ofLolium species and cultivars by rapid SDS-PAG electrophoresis of seed storage proteins.—Seed Sci. Technol.21: 213–223, 1993.

    Google Scholar 

  • Munthali, M., Ford-Lloyd, B.V., Newbury, H.J.: The random amplification of polymorphic DNA for fingerprinting plants.—PCR Method Appl. 1: 274–276, 1992.

    CAS  Google Scholar 

  • Murphy, P.M., Culleton, N., Flaherty, T.: Identification of grass seed cultivars by SDS polyacrylamide gel electrophoresis.—Irish J. agr. Res.29: 117–127, 1990.

    Google Scholar 

  • Nei, M., Li, W.H.: Mathematical model for studying genetic variation in terms of restriction endonucleases.—Proc. nat. Acad. Sci. USA76: 5269–5273, 1979.

    Article  PubMed  CAS  Google Scholar 

  • Ostergaard, H., Nielsen, G., Johansen, H.: Genetic variation in cultivars of diploid ryegrassLolium perenne andL. multiflorum at five enzyme loci.—Theor. appl. Genet.69: 409–421, 1985.

    Article  Google Scholar 

  • Perez-Vicente, R., Petris, L., Osusky, M., Potrykus, I., Spangenberg, G.: Molecular and cytogenetic characterization of repetitive DNA sequences fromLolium andFestuca: applications in the analysis ofFestulolium hybrids.—Theor. appl. Genet.84: 145–154, 1992.

    Article  CAS  Google Scholar 

  • Samec, P.: DNA polymorphism and RAPD technology. A review.—Genet. Šlechtění (Praha)29: 291–320, 1993.

    Google Scholar 

  • Tingey, S.V., del Tufo, J.P.: Genetic analysis with random amplified polymorphic DNA markers.— Plant Physiol.101: 349–352, 1993.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wiesner, I., Samec, P. & Našinec, V. Identification and relationships of cultivated accessions fromLolium-Festuca complex based on RAPD fingerprinting. Biol Plant 37, 185–195 (1995). https://doi.org/10.1007/BF02913208

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02913208

Key words

Navigation