Abstract
The green fluorescent protein (GFP) gene from the jellyfishAequorea victoria as a vital reporter for gene expression in plants is considered to have several advantages over other reporter genes. The pBIN35S-mGFP4 plasmid DNA has been introduced into cotton embryos by the pollen-tube pathway method. A transformed seedling has been verified according to its GFP-related fluorescence and Southern blotting analysis. The results provided direct and convincing facts in cytology and molecular biology for the pollen-tube pathway method, an efficient transformation technique used in plants.
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Huang, G., Dong, Y. & Sun, J. Introduction of exogenous DNA into cotton via the pollen-tube pathway with GFP as a reporter. Chin.Sci.Bull. 44, 698–701 (1999). https://doi.org/10.1007/BF02909705
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DOI: https://doi.org/10.1007/BF02909705