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The effect of dehydration and rehydration on the nitrogen content of various fractions from resurrection plants

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Biologia Plantarum

Abstract

Nitrogen contents were determined in 20 species of “resurrection plants”,i.e. plants with leaves which are able to revive from an air-dry state (viz. Boea hygroscopica, Borya nitida, Cheilanthes sieberi, Coleochloa pallidior, C. setifera, Craterostigma plantagineum, Myrothamnus flabellifolia, Oropetium capense, Pellaea calomelanos, P. falcata, P, viridis, Polypodium polypodioides, Ramondia pyrenaica, Selaginella lepidophylla, Sporobolus stapfianus, Talbotia elegans,Tripogon loliiformis, Xerophyta retinervis, X. villosa, X. viscosa), and in three desiccation sensitive species (Eragrostis tenuifolia, Selaginella kraussiana andSporobolus pyramidalis).

In a preponderance of resurrection plants insoluble nitrogen content fell during dehydration of intact plants and soluble non-protein N rose. Both changes were particularly marked in species which lose chlorophyll and thylakoid structure during drying. These trends were usually only partially reversed after 24 h rehydration. Recovery of14C-leucine incorporation in rehydrating leaves was slow.

Leaves of desiccation sensitive vascular plants tended on the average to lose soluble protein rather than insoluble N during drying, and tended to have higher soluble non-protein N contents than tolerant plants.

However, similarity in the changes in N-contents inXerophyta villosa leaves killed by airdrying compared to leaves surviving air-drying, opposes the view that death was due to excessive loss of protein.

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Abbreviations

EM:

electron microscope

NCS:

NCS tissue solubilizer, Amersham/Searle, Illinois

POPOP:

l,4-bis-2(4-methyl-5-phenyl)oxazolylbenzene

PPO:

2,5-diphenyloxazole

1 μCi:

37 kBq

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Authors and Affiliations

  1. Botany Department, Monash University, 3168, Clayton, Victoria, Australia

    D. F. Gaff & G. R. McGregor

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  1. D. F. Gaff
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  2. G. R. McGregor
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Gaff, D.F., McGregor, G.R. The effect of dehydration and rehydration on the nitrogen content of various fractions from resurrection plants. Biol Plant 21, 92–99 (1979). https://doi.org/10.1007/BF02909453

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  • DOI: https://doi.org/10.1007/BF02909453

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