Abstract
Schizosaccharomyces pombe, growing in a complex medium at 32°C, was treated with 6 or 7 heat shocks (30 min, 41°C) spaced one normal cell generation apart (110 min, 32°C). This treatment results in synchronization of cell division, nuclear division, and DNA synthesis.
Between successive shocks increases in the activities of ATCase and OTCase are stepwise. However, steps are not seen at constant temperature, neither in free running heat synchronized, nor in selection synchronized cells. The enzyme steps we have studied therefore seem to be directly induced by the temperature shocks, and they are dissociable from the pattern of classic cell cycle events, which we find almost identical in heat and in selection synchronized cells.
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Kramhøft, B., Nissen, S.B. & Zeuthen, E. The cell cycle in heat- and selection-synchronized schizosaccharomyces pombe. Carlsberg Res. Commun. 41, 15–25 (1976). https://doi.org/10.1007/BF02908690
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DOI: https://doi.org/10.1007/BF02908690