Abstract
The polyene antibiotic N-glycosyl-polifungin has been used as an effective counterselective agent for biochemical mutants in Mucor. Treatment with this antibiotic has resulted in at least 40-fold enrichment of auxotrophic mutants in a mutagenised spore population of M. circinelloides. The mutants obtained from this species belong to at least six different phenotypic classes, most of them not previously described. This is the first report of the isolation of amino acid auxotrophs from M. miehei.
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Abbreviations
- NPG:
-
N-glycosyl-polifungin
References
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Accepted by:H. Klenow, E. Lund andS.O. Andersen
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Roncero, M.I.G. Enrichment method for the isolation of auxotrophic mutants of Mucor using the polyene antibiotic N-glycosyl-polifungin. Carlsberg Res. Commun. 49, 685–690 (1984). https://doi.org/10.1007/BF02907499
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DOI: https://doi.org/10.1007/BF02907499