Abstract
The authors describe an efficient method for generating large deletions (>200 nts) of precise length using the PCR-based method of gene splicing by overlap extension (1). This method is technically simple and less time consuming than conventional loop-out mutagenesis techniques requiring preparation of a single-stranded DNA template.
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Senanayake, S.D., Brian, D.A. Precise large deletions by the PCR-based overlap extension method. Mol Biotechnol 4, 13–15 (1995). https://doi.org/10.1007/BF02907467
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DOI: https://doi.org/10.1007/BF02907467