Abstract
The relationship between the viscosity of an acid flour extract and the acid-soluble, acid-insoluble and total β-glucan content of barley flour has been investigated. The extract viscosity was found to be closely related (R2=0.99, S.E.E.=0.19) to the content of soluble β-glucan. It is concluded that the acid-soluble β-glucans from 14 of the 18 different genotypes studied have a similar structural nature. Genotypes containing the lys-3a allele were found to have very low extract viscosities and β-glucan contents. Quick and accurate methods are described for measuring viscosities of acid extracts made from flour derived from grains of a single plant and flour derived from a single grain. These methods can be used to screen plants for their soluble β-glucan content in a plant breeding program.
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Allison, M. J., I. A. Cowe &R. McHale: The use of infra red reflectance for the rapid estimation of the soluble β-glucan content of barley. J. Inst. Brew. 84, 153–155 (1978)
Anderson, R. L., M. A. Anderson & B. A. Stone: Some structural enzymatical aspects of nonstarchy polysaccharides in cereal grains. Inst. Brew. Aust. N.Z.Sec. Proc. 14th conv. 153–160 (1976)
Anderson, M. A., J. A. Cook &B. A. Stone: Enzymatic determination of 1,3∶1,4 β-glucans in barley grains and other cereals. J. Inst. Brew. 84, 233–239 (1978)
Aspinal, G. O. &C. T. Greenwood: Aspects of the chemistry of cereal polysaccharides. J. Inst. Brew. 68, 167–177 (1962)
Barrett, J., J. F. Clapperton, D. M. Divers &H. Rennie: Factors affecting wort separation. J. Inst. Brew. 79, 407–413 (1973)
Bathgate, G. N. &C. E. Dalgliesh: The diversity of barley and malt beta-glucans. ASBC Proceedings 33, 32–36 (1975)
Bendelow, V. M.: Determination of non-starch polysaccharides in barley breeding programmes. J. Inst. Brew. 81, 127–130 (1975)
Bourne, D. T., M. Jones &J. S. Pierce: β-glucan and β-glucanase in malting and brewing. MBAA Technical Quarterly 13, 3–7 (1976)
Bourne, D. T. &J. S. Pierce: β-glucan and β-glucanase in brewing. J. Inst. Brew. 76, 328–335 (1970)
Burnett, G. S.: Studies of viscosity as the probable factor involved in the improvement of certain barleys for chickens by enzyme supplementation. British Poultry Science 7, 55–75 (1966)
Clarke, A. E. &B. A. Stone: Chemistry and biochemistry of 1–3-glucans. Rev. Pure Appl. Chem. 13, 134–156 (1963)
Dalgliesh, C. E. &J. R. Hudson: Research Foundation Report. J. Inst. Brew. 84, 61–62 (1978)
Djurtoft, R. &K. L. Rasmussen: Studies on barley gum preparations including determination of the molecular weight. EBC Proc. 17, 17–25 (1955)
Dubois, M., K. Gilles, J. K. Hamilton, P. A. Rebers &F. Smith: Colorimetric method for determination of sugars and related substances. Anal. Chem. 28, 350–356 (1956)
Erdal, K. & P. Gjertsen: Beta-glucans in malting and brewing II. The fate of beta-glucans during mashing. EBC Proc. 11th congr. 295–302 (1967)
Erdal, K. & P. Gjertsen: Beta-glucans in malting and brewing III. The action of endo-beta-glucanases. EBC Proc. 13th congr. 49–57 (1971)
Ewertson, G.: Protein content and grain quality relations in barley. Agri Hortique Genetica. band XXXV, 58–61 (1977)
Fincher, G. B.: Morphology and chemical composition of barley endosperm cell walls. J. Inst. Brew. 81, 116–122 (1975)
Flemming, M., D. J. Manners, R. M. Jackson &S. C. Cooke: The estimation of β-glucans in barley. J. Inst. Brew. 80, 399–404 (1974)
Flemming, M. &K. Kawakami: Studies of the fine structure of β-D-glucans of barley extracted at different temperatures. Carbohydrate Research 57, 15–23 (1977)
Flory, P. J.: Principles of polymer chemistry. VII–4a, pp. 308–311, Cornell University Press, Ithaca, New York (1953)
Forrest, I. S. & T. Wainwright: Differentiation between desirable and troublesome β-glucans. Eur. Brew. Conv. Congr. pp. 401–412 (1977)
Forrest, I. S. &T. Wainwright: The mode of binding of β-glucans and pentosans in barley endosperm cell walls. J. Inst. Brew. 83, 279–286 (1977)
Gjertsen, P.: Filtration of beer and factors influencing its filterability. Brew. Dig. XLV, 68–80 (1970)
Gjertsen, P.: Beta-glucans in malting and brewing. I. Influence of beta-glucans on the filtration of strong beers. ASBC. Proc. 113–120 (1966)
Gohl B., S. Alden, K. Elwinger &S. Thomke: Influence of β-glucanase on the feed value of barley for poultry and moisture content of excreta. Br. Poult. Sci. 19, 41–47 (1978)
Gohl, B. &I. Gohl: The effect of viscous substances on the transit time of barley digesta in rats. J. Sci. Food. Agric. 28, 991–1006 (1977)
Greenberg, D. C.: β-glucan and extract viscosity. J. Inst. Brew. 80, 435 (1974)
Greenberg, D. C. &E. T. Whitmore: A rapid method for estimating the viscosity of barley extracts. J. Inst. Brew. 80, 31–33 (1974)
Jensen, J. &H. Doll: Gene symbols for barley high-lysine mutants. Barley Genetics Newsletter 9, 33–37 (1979)
Koszyk, P. F. &M. J. Lewis: Unmalted grains as malters' adjuvant and brewers' adjunct. J. Am. Soc. Brew. Chem. 37, 77–81 (1977)
Meilgaard, M. C.: Wort composition: With special reference to the use of adjuncts. MBAA Technical Quarterly 13, 78–90 (1976)
Meredith, W. O. S.: The significance of malt analysis in industry and plant breeding. Inst. Brew. Aust. N.Z. Sec. Proc. 13th Conv. 29–40 (1974)
Morrall, P. &D. E. Briggs: Changes in cell wall polysaccharides of germinating barley grains. Phytochemistry, 17, 1495–1502 (1978)
Morgan, A. G.: The relationship between barley extract viscosity curves and malting ability. J. Inst. Brew. 83, 231–234 (1977)
Morgan, A. H. &P. G. Gothard: A rapid, simple viscometric technique for indirect estimation of soluble β-glucan content of raw barley. J. Inst. Brew. 83, 37–38 (1977)
Morgan, K.: Heat lability of barley β-glucan. J. Inst. Brew. 77, 509 (1971)
Munck, L.: High lysine barley—a summary of the present research development in Sweden. Barley Genetics Newsletter 2, 54–59 (1972)
Palmer, G. H.: Influence of endosperm structure on extract development. ASBC Proc. 33, 174–180 (1975)
Polyakov, V. A., V. N. Ugryumova &I. Ya. Veselov: Determination of β-glucan content in barley and malt. Fermentn. Spirt. Prom-st. 7, 38–39 (1976)
Preece, I. A. &K. G. Mackenzie: Non-starchy polysaccharides of cereal grains I. Fractionation of the barley gums. J. Inst. Brew. 58, 353–362 (1952)
Preece, I. A. &K. G. Mackenzie: Non-starchy polysaccharides of cereal grains II. Distribution of water-soluble gum-like materials in cereals. J. Inst. Brew. 58, 457–464 (1952)
Scott, R. W.: The viscosity of worts in relation to their contents of β-glucans. J. Inst. Brew. 78, 179–186 (1972)
Schuster, K., L. Narziss &J. Kumada: Über die Gummistoffe der Gerste und ihr Verhalten während der Malz- und Bierbereitung. I. Problemstellung und Analysemetoden. Brauwissenschaft 20, 125–135 (1967)
Schulster, K., L. Naziss &J. Kumada: Über die Gummistoffe der Gerste und ihr Verhalten während der Malz- und Bierbereitung. II. Gummistoffe in der Gerste und ihre Veränderungen beim Mälzen und Maishen. Brauwissenschaft 20, 185–206 (1967)
Smart, J. G.: The β-glucan content of New Zealand barley. Inst. Brew. Aust. N.Z. sec. Proc. 14th Conv. 161–168 (1976)
Smith, D. B., A. G. Morgan & A. A. Gill: The major biochemical constituents of an acid flour extract of barley flour as used in viscosity determinations by the falling ball method. J Inst. Brew. (in press) (1979)
Sparrow, D. H. G. &W. O. S. Meredith: Malt cytolytic activity of barley of diverse origins and its relation to maltability. J. Inst. Brew. 75, 237–242 (1969)
Tanford, C.: Physical chemistry of macromolecules. Chap. 6, 19f, pp. 328–329, John Wiley & sons, inc., New York, London (1961)
Thompson, R. G. &D. E. LaBerge: Barley endosperm cell walls: A review of cell wall polysaccharides and cell wall-degrading enzymes. MBAA Technical Quarterly 14, 238–243 (1977)
Wainwright, T.: Barley analysis and malt quality. MBAA Technical Quarterly 15, 184–189 (1978)
Wainwright, T. &G. K. Buckee: Barley and malt analysis—a review. J. Inst. Brew. 83, 325–347 (1977)
Wood, P. J., D. Paton &I. R. Siddiqui: Determination of β-glucan in oats and barley. Cereal Chem. 54, 524–533 (1977)
Wood, P. J., I. R. Siddiqui &D. Paton: Extraction of high-viscosity gums from oats. Cereal Chem. 55, 1038–1049 (1978)
Yang, J. T.: Adv. Protein Chem. 16 IIB, pp. 328–239, Academic Press, New York, London (1961)
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Aastrup, S. The relationship between the viscosity of an acid flour extract of barley and its β-glucan content. Carlsberg Res. Commun. 44, 289–304 (1979). https://doi.org/10.1007/BF02906492
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DOI: https://doi.org/10.1007/BF02906492