Abstract
In partly purified protein complexes obtained from 22 species of theAllium genus and 6 cultivars ofAllium cepa the activity of cholinesterases was detected and measured using the method of Ellman et al. The degree of its inhibition with 10-4 M neostigmine was also tested. It was found that the activity of cholinesterase differed in individual species up to two hundred times, while the differences in the inhibitory activity of 10-4 M neostigmine occurred only in a few cases. Individual sections and cultivars could not be characterized on the basis of the differences in the activities of the cholinesterases. Of all the sections that ofPhyllodolon shows the highest average activity. In the case of the tested cultivars distinctly the lowest activity was observed in cv. Kastická. The values of the enzymatic activity measured by Ellman’s method in this plant material include the activity of specific and unspecific cholinesterases and the part uninhibitable by neostigmine.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bradford, M. M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. - Anal. Biochem.72: 248–254, 1976.
Ellman, G. L., Courtney, K. D., Andres, V., Featherstone, R. M.: A new and rapid colorimetric determination of acetylcholinesterase activity. - Biochem. Pharmacol.7: 88–95, 1961.
Ernst, M., Hartmann, E.: Biochemical characterization of an acetylcholinehydrolyzing enzyme from bean seedlings. -Plant Physiol.65: 447–450, 1980.
Fluck, R. A., Jaffé, M. J.: The acetylcholine system in plants. - Curr. Adv. Plant. Sci.5: 1–22, 1974a.
Fluck, R. A., Jaffé, M. J.: The distribution of cholinesterases in plant species. - Phyto-chemistry13: 2475–2480, 1974b.
Goryachenko, E. V.: [An enzyme from garlic, producing allicin (allicinase)-proteid of phospho-pyridoxal.] In Russ. - Dokl. Akad. Nauk SSSR87: 457–480, 1952.
Gupta, R., Maheshwari, S. C.: Preliminary characterization of a cholinesterase from roots of Bengal gram -Cicer arietinum L. - Plant Cell Physiol.21: 1675–1679, 1980.
Hadačová, V., Hofman, J., Almeida, R. M., Vackovà, K., Kutáček, M., Klozová, E.: Choline esterases and choline acetyltransferase in the seeds ofAllium altaicum (Pall.) Reyse. - Biol. Plant.23: 220–227, 1981a.
Hadačová, V., Klozová, E., Hadač, E., Turková, V., Pitterová, K.: Comparison of esterase isoenzyme patterns in seeds of someAllium species and in cultivars ofAllium cepa L. - Biol. Plant.23: 174–181, 1981b.
Kasturi, R., Vasantharajan, V. N.: Properties of acetylcholinesterase fromPisum sativum. -Phytochemistry15 : 1345–1347, 1976.
Klozová, E., Hadačová, V., Turková, V.: A contribution to the standardization of methods for the preparation of seed proteins ofAllium cepa L. - Biol. Plant.21: 284–290, 1979.
Mansfield, D. H. G., Clark, D. G., Taylor, I. E. P.: Partial purification and some properties of a cholinesterase from bush bean (Phaseolus vulgaris L.) roots. - Biochem. J.175: 769–777, 1978.
Pearse, A. G. E.: Histochemistry, Theoretical and Applied (3. ed.). - Churchill Livingstone, Edinburgh -London 1972.
Riddles, P. W., Blakeley, R. L., Zerner, B.: Ellman’s reagent 5,5′-dithiobis (2-nitrobenzoic acid) — a reexamination. - Anal. Biochem.94: 75–81, 1979.
Riov, J., Jaffé, M. J.: A cholinesterase from bean roots and its inhibition by plant growth retardants. -Experientia29: 264–265, 1973a.
Riov, J., Jaffé, M. J.: Cholinesterase from plant tissues. I. Purification and characterization of a cholinesterase from mung bean roots. - Plant Physiol.51: 520–528, 1973b.
Selby, C., Galpin, I. J., Collin, H. A.: Comparison of the onion plant (Allium cepa) and onion tissue culture. Aliinase activity and flavour precursor compounds. - New Phytol.83: 351 to 359, 1979.
Verheul, F. E. A. M., Draijer, J. W., Muijser, A. O., Van Gelder, B. F.: The reactivity of thiol groups in bovine heart cytochrome c oxidase towards 5,5′-dithiobis (2-nitrobenzoic acid). - Biochim. biophys. Acta681: 118, 1982.
Voss, G.: Taxonomy related cholinesterase inhibition patterns in insects. - J. econ. Entomol.74 : 555–557, 1981.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hadačová, V., Vacková, K., Klozová, E. et al. Cholinesterase activity in some species of theAllium genus. Biol Plant 25, 209–215 (1983). https://doi.org/10.1007/BF02902105
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02902105