Skip to main content
SpringerLink
Log in

Preparation of magnetic gelatin microspheres for the targeting of drugs

  • Original Articles
  • Published:
Archives of Pharmacal Research Aims and scope Submit manuscript

Abstract

Magnetically responsive gelatin microspheres for the targeting of drugs have been prepared using a water-in-oil emulsion technique with chemical cross-linking of the protein. The manufacturing variables affecting microsphere size, size distribution and surface characteristics have been examined as well as the magnetic responsivenessin vitro.

Sesame oil was utilized for non-aqueous phase and magnetic gelatin microspheres of different size from 1.89 to 14.88 μm in mean diameter could be obtained with variation of HLB values of non-ionic surfactants. The content of magnetite which uniformly distributed throughout the microspheres was 26.7% (w/w). It was possible to control the localization of magnetic gelatin microspheres at specific sites within capillary models by using external magnetic field of under 5K gauss.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  1. Goldberg, E.P.: “Targeted Drugs”, John Wiley & Sons, New York, p. 296 (1983).

    Google Scholar 

  2. Gregoriadis, G., Senior, D. and Trouet, A.: “Targeting of Drugs”, Plenum Press, New York, p. 419 (1982).

    Google Scholar 

  3. Schacht, E., Ruys, Vermeersch, J., Remon, J.P. and Duncan, R.: Use of polysaccharides as drug carriers.Annals. N.Y. Acad. Sci.,446, 199 (1985).

    Article  CAS  Google Scholar 

  4. Bodor, N.: Targeting of drugs to the brain, in “Drug and Enzyme Targeting” (Widder, K. J. and Green R. ed), Academic Press, London, p. 381 (1985).

    Chapter  Google Scholar 

  5. Gregoriadis, G. and Allison, A.C. “Liposomes in biological systems”, John Wiley & Sons, New York, p. 412 (1980).

    Google Scholar 

  6. Davis, S.S. and Illum, L.: The targeting of drugs using polymeric microspheres.British Polymer J.,15, 160 (1983).

    Article  CAS  Google Scholar 

  7. Oppenheim, R.C.: Solid colloidal drug delivery systems.Int. J. Pharm.,8, 217 (1981).

    Article  CAS  Google Scholar 

  8. Gregoriadis, G.: “Drug Carriers in Biology and Medicine”, Academic Press, London, p. 363 (1981).

    Google Scholar 

  9. Widder, K.J. and Senyei, A.E.: Magnetic microspheres: A vehicle for selective targeting drugs.Pharmac. Ther.,20, 377 (1983).

    Article  CAS  Google Scholar 

  10. Morimoto, Y., Okumua, M., Sugibayashi, K. and Kato, Y.: Biomedical applications of magnetic fluids. II. Preparation and magnetic guidance of magnetic albumin microsphere for site specific drug deliveryin vivo.J. Pharm. Dyn.,4, 624 (1981).

    CAS  Google Scholar 

  11. Tanaka, T., Takino, S. and Utsumi, I.: A new gelatinized sustained-release dosage form.J. Pharm. Sci.,52, 664 (1963).

    Article  PubMed  CAS  Google Scholar 

  12. Scheffel, U., Rhodes, B.A., Natarajan, T.K. and Wagner, Jr., N.N.: Albumin microspheres for study of the reticuloendothelial system.J. Nucl. Med.,13, 498 (1972).

    PubMed  CAS  Google Scholar 

  13. Oppenheim, R.C.: Nanoparticles, in “Drug Delivery System” (Juliano, R.L. ed.), Oxford Univer. Press, Oxford, pp. 177 (1980).

    Google Scholar 

  14. Sezaki, H., Hashida, M. and Muranishi, S.: Gelatin microspheres as carriers for antineoplastic agents, in “Optimization of Drug Delivery” (Bundgaard, H., Hansen, A.B. and Kofod, H. eds.), Munksgaard, Copenhagen, pp. 316 (1982).

    Google Scholar 

  15. Morimoto, Y.: Personal communication.

  16. Jizomoto, H.: Phase separation induced in gelatin-base coacervation systems by addition of water-soluble nonionic polymers I: Microencapsulation.J. Pharm. Sci.,73, 879 (1984).

    Article  PubMed  CAS  Google Scholar 

  17. Kramer, P.A.: Albumin microspheres as vehicles for achiving specificity in drug delivery.J. Pharm. Sci.,63, 1646 (1974).

    Article  PubMed  CAS  Google Scholar 

  18. Gallo, J.M., Hung, C.T. and Perrier, D.G.,: Analysis of albumin microsphere preparation.Int. J. Pharm.,22, 63 (1984).

    Article  CAS  Google Scholar 

  19. Dunn, A.S.: Effects of the choice of emulsifier in emulsion polymerization, in “Emulsion Polymerization” (Piirma, I. ed.), Academic Press, New York, pp 221 (1982).

    Google Scholar 

  20. Marty, J.J., Oppenheim, R.C. and Speiser, P.: Nanoparticles-a new colloidal drug delivery system.Pharm. Acta Helv.,53, 17 (1978).

    PubMed  CAS  Google Scholar 

  21. Ycshioka, T., Hashida, M., Muranishi, S. and Sezaki, H.: Specific delivery of Mitomycin C to the liver, spleen and lung: Nano and microspherical carriers of gelatin.Int. J. Pharm.,8, 131 (1981).

    Google Scholar 

  22. Eberth, K. and Merry, J.: A comparative study of emulsions prepared by ultrasound and by a conventional method. Droplet size measurements by means of a coulter counter and microscopy.Int. J. Pharm.,14, 349 (1983).

    Article  CAS  Google Scholar 

  23. Tomlinson, E.: Microsphere delivery systems for drug targeting and controlled release.Int. J. Pharm. Technol. Prod. Manuf.,4, 49 (1983).

    CAS  Google Scholar 

  24. Oppenheim, R.C.: Solid colloidal drug delivery systems.Int. J. Pharm.,8, 217 (1981).

    Article  CAS  Google Scholar 

  25. Willmott, N., Cummings, J., Stuart, J.F.B. and Florence, A.T.: Adriamycin-loaded albumin microspheres: Preparation,in vivo distribution and release in the rat.Biopharm. & Drug Disposition,6, 91 (1985).

    Article  CAS  Google Scholar 

  26. McMullen, J.N., Newton, D.W. and Becker, C.H.: Pectin-gelatin complex coacervates II: Effect of microencapsulated sulfamerzine on size, morphology, recovery, and extraction of water-dispersible microglobules.J. Pharm. Sci.,73, 1799 (1984).

    Article  PubMed  CAS  Google Scholar 

  27. Illum, L. and Davis, S.S.: Effect of the nonionic surfactant poloxamer 338 on the fate and deposition of polystyrene microspheres following intravenous administration.J. Pharm. Sci. 72, 1086 (1983).

    Article  PubMed  CAS  Google Scholar 

  28. Ghose, T., Blair, A.H., Uadia, P., Kulkarni, P.N., Goundalkar, A., Mezei, M. and Ferrone, S.: Antibodies as carriers of cancer chemotherapeutic agents.Annals N.Y. Acad. Sci.,446, 213 (1985).

    Article  CAS  Google Scholar 

  29. Treleaven, J.G., Gibson, F.M., Ugelstad, J., Rembaum, A., Philip, T., Caine, G.D. and Kemshead, J.T.: Removal of neuroblastoma cells from bone marrow with monoclonal antibodies conjugated to magnetic microspheres.The Lancet, Jan.14, 70 (1984).

    Article  Google Scholar 

  30. Whitmore, R.L.: “Rheology of the circulation”, Pergamon Press, Oxford, (1968).

    Google Scholar 

  31. Allen, T., “Particle size measurements”, 3rd edn., Chapman and Hall, London, p. 127 (1981).

    Google Scholar 

  32. Kato, T.: Encapsulated drugs in targeted cancer therapy. in “Controlled Drug Delivery” Vol. II (Bruck, S.D. ed.), CRC Press, Boca Raton, pp. 189 (1983).

    Google Scholar 

  33. Fujimoto, S., Miyazaki, M., Enndoh, F., Takahasi, O., Shrestha, R.D., Okui, K., Morimoto, Y. and Terao, K.: Effects of intra-arterially infused biodegradable microspheres containing Mitomycin C.Cancer,55, 522 (1985).

    Article  PubMed  CAS  Google Scholar 

  34. Ratcliffe, J.H., Hunneyball, I.M., Smith, A., Wilson, C.G. and Davis, S.S.: Preparation and evaluation of biodegradable polymeric systems for the intra-articulr deliverry of drugs.J. Pharm. Pharmacol.,36, 431 (1984).

    PubMed  CAS  Google Scholar 

  35. Widder, K.J., Senyei, A.E., Ovadiaa, H. and Paterson, P.Y.: Specific cell binding using Staphylococcal Protein A magnetic microspheres.J. Pharm. Sci.,70, 387 (1981).

    Article  PubMed  CAS  Google Scholar 

  36. Burgess, D.J. and Carless, J.E.: Manufacture of gelatin/gelatin coacervate microcapsules.Int. J. Pharm.,27, 61 (1985).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Pharmacy, Chonnam National University, 500, Gwangju, Korea

    Kang Choon Lee, Ik Bae Koh & In Joon Oh

Authors
  1. Kang Choon Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ik Bae Koh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. In Joon Oh
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lee, K.C., Koh, I.B. & Oh, I.J. Preparation of magnetic gelatin microspheres for the targeting of drugs. Arch. Pharm. Res. 9, 145–152 (1986). https://doi.org/10.1007/BF02899998

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02899998

Keywords

Search

Navigation