Summary
Isolated follicular dendritic cells (FDCs) showed true and pseudoemperipolesis of fresh tonsillar lymphocytes, even after long-term (50-day) cultivation. Emperipolesis by FDCs was not restricted by allotype specificity, nor was it inhibited by the addition of antibodies against MHC-I & II antigens. Follicular dendritic cells predominantly engulfed B-cells; monocytes and macrophages were not found between FDC cytoplasmic extensions. When highly purified T-cell populations were added to FDC cultures emperipolesis of T-cells occurred, particularly those of the CD4-positive phenotype. Mitoses appeared within 6 h in the emperipolesed lymphocytes and, after an additional 18 h, some lymphocytes exhibited apoptosis.
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Tsunoda, R., Nakayama, M., Heinen, E. et al. Emperipolesis of lymphoid cells by human follicular dendritic cells in vitro. Virchows Archiv B Cell Pathol 62, 69–78 (1992). https://doi.org/10.1007/BF02899667
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DOI: https://doi.org/10.1007/BF02899667