Summary
In order to clarify the function of human S100β- positive T-cells, S100β-positive T-leukemia cells (S100β TLC) were examined in vitro. S100β TLC were obtained from the peripheral blood of a patient with S100β-positive T-cell leukemia and enriched by an E-rosetting method. Two dimensional flow cytometric analysis indicated that the vast majority of the E-positive fraction were S100β TLC expressing CD3 and CD8 antigens. Although S100β TLC expressed CD3 antigen, they were negative for the α/β and γ/δ T-cell antigen receptor (TCR) defined by monoclonal antibodies (mabs) WT-31 and δ TCS-1, respectively. It was speculated that S100β TLC initially expressed α/β TCR but lost it during malignant transformation. When S100β TLC were cultured for 24 h, they acquired cytotoxic activity towards various NK-sensitive cell lines including K-562, Molt-3 and CEM-CCLF, but did not exhibit lysing activity towards NK-resistant cell lines including Raji, Daudi and MT-1. Despite the NK-activity of cultured S100β TLC, they lacked the morphological features of large granular lymphocytes (LGL). S100β TLC did not exhibit lymphokine-activated killer (LAK) activity. When S100β TLC were cocultivated with NK-sensitive cells or NK-resistant cells, they selectively bound to NK-sensitive cells, indicating that they lysed target cells by cell-to-cell contact. The finding that S100 β TLC lacked TCR molecules and their NK activity was not inhibited by mabs reactive with the CD3-TCR complex indicated that the CD3-TCR complex was not involved in their target recognition. These findings suggest that S100 β-positive T-cells are functionally similar to NK cells. We discuss the roles of S100 β-positive T-cells in the human immune system.
Similar content being viewed by others
References
Allavena P, Introna M, Rambald A, Zanaboni F, Rossini S, Villa A, Bassan R, Barbui T, Mantovani A (1986) Induction of cytotoxicity by interleukin-2 in T γ-lymphoproliferative disorders. Int J Cancer 37:27–33
Borst J, van de Griedn RJ, van Oostveen JW, Ang S-L (1987) A T cell receptor γ/CD3 complex found on cloned functional lymphocytes. Nature 325:638–688
Brenner MB, Trowbridge IS, Strominger JL (1985) Cross-linking of human T-cell receptor proteins: Association between the Tcell indiotypeβ subunit and the T3 glycoprotein heavy subunit. Cell 40:183–190
Brooks CG, Holscher M (1987) Cell surface molecules involved in NK recognition by cloned cytotoxic T lymphocytes. J Immunol 138:1331–1337
Chen JKCNCS, Chu YC, Wong KF (1987) S-100 positive sinusoidal large cell lymphoma. Human Pathol 18:756–759
Cocchia D, Michetti F, Donato R (1981) Immunochemical and immunocytochemical localization of S100 antigen in normal human skin. Nature 294:85–87
Dennert G, Anderson CG, Prochazka G (1987) High activity of Nα-benzyloxycarbonily-L-lysine thiobenzyl ester serine esterase and cytolytic perforin in cloned cell lines is not demonstrable in in vivo-induced cytotoxic effector cells. Proc Natl Acad Sci USA 84:5004–5008
Groscurth P, Qiao B, Podack ER, Hengartner H (1987) Cellular localization of perforin 1 in murine cloned cytotoxic T lymphocytes. J Immunol 138:2749–2752
Isobe T, Okuyama T (1978) The amino acid sequence of S100 protein (PAP-1b) protein and its relation to calcium binding proteins. Eur J Biochem 89:379–388
Knowles DM (1986) The Human T-cell leukemia: Clinical, cytomorphologic, immunophenotypic, and genotypic characteristics. Human Pathol 17:14–33
Merle-Beral H, Boucheix C, Karray S, Hercend T, Capron F, Autran B, Vazquez A, Blanc C, Oberling F, Debre P (1987) A T chronic leukemia with large granular lymphocytes. Phenotype and finctions of leukemic cells under in vitro treatment by differentiation inducers. Cancer 59:1296–1303
Moore BW (1965) A soluble protein characteristic of the nervous system. Biochem Biophys Res Commun 19:739–744
Morio T, Nonoyama S, Yata J (1990) Suppression of in vitro Ig synthesis by CD16 (Leulla)+ CD56 (NKH1, Leu19)+ non-T lineage NK cells and the lack there of in cells from immunodeficient patients. Clin Exp Immunol (in press)
Motoi M, Yoshino T, Nose S, Akagi T, Nakamoto S, Takahashi K (1987) Three cases with S-100 positive malignat lymphoma (Abstr in Japanese). J Jpn Soc Reticuloendothel Sys 27:111
Nakanishi N, Maeda K, Ito K, Heller M, Tonegawa S (1987) T γ protein is expressed on murine fetal thymocytes as a disulphide-linked heterodimer. Nature 325:720–723
Ostergaard HL, Kane KP, Mescher MF, Clark WR (1987) Cytotoxic T lymphocyte mediated lysis without release of serine esterase. Nature 330:71–72
Panfilis GD, Rowden G, Manara G, Ferrari C, Torresani C, Sansoni P (1988) The S100β protein in normal human peripheral blood is uniquely present within a discrete suppressor T-cell compartment. Cell Immunol 114:398–404
Picker LJ, Brenner MB, Michie S, Warnke RA (1988) Expression of T-cell receptor delta chains in benign and malignant T lineage lymphoproliferations. Am J Pathol 132:401–405
Phillips JH, Weiss A, Gemlo BT, Rayner AA, Lanier LL (1987) Evidence that the T-cell antigen receptor may not involved in cytotoxicity mediated by γ/δ and α/β thymic cell lines. J Exp Med 166:1579–1584
Ruco LP, Stoppaciaro A, Barosotti P, Vitro D, Mirolo M, Cassano AM, Guglielum C, Mandelli F, Uccini S, Baroni CD (1984) S100+ lymph node neoplasm. Report of a case with histologically AL and immunological features intermediate between T cell lymphoma and malignant histiocytosis. Virchows Arch [A] 404:351–358
Sansoni P, Rowden G, Manara GC, Ferrari C, Panfilis G (1987) S-100 positive T cells are largely restricted to a CD8-positive, 9.3 negative subset. Virchows Arch [B] 53:301–307
Schmidt RE, Murray C, Daley JF, Schlossman SF, Rits J (1986) A subset of natural killer cells in peripheral blood displays a mature T cell phenotype. J Exp Med 164:351–356
Takahashi K, Yamaguchi H, Ishizeki J, Nakajima T, Nakazato Y (1981) Immunohistochemical and immunoelectron microscopic localization of S100 protein in the interdigitating reticulum cells of the human lymph nodes. Virchows Arch [B] 37:125–135
Takahashi K, Isobe T, Ohtsuki Y, Sonobe H, Akagi T, Okuyama T (1984) Immunohistochemical study on the distribution of α andβ subunits of S100 protein in the human normal and neoplastic tissues. Virchows Arch [B] 45:383–396
Takahashi K, Isobe T, Ohtsuki Y, Sonobe H, Yamaguchi H, Akagi T (1985) S100 protein positive human T-lymphocyte. Am J Clin Pathol 83:69–72
Takahashi K, Ohtsuki Y, Sonobe H, Hayashi K (1987a) Immunocytochemical characterization of S100β positive human T lymphocytes by a double immunostaining method. Virchows Arch[B] 53:375–377
Takahashi K, Yoshino T, Hayashi K, Sonobe H, Ohtsuki Y (1987b) S100 beta positive human T-lymphocytes: Their characteristics and behavior under normal and pathologic conditions. Blood 70:214–220
Takahashi K, Ohtsuki Y, Sonobe H, Hayashi K, Nakamura S, Kotani S, Kubonishi I, Miyoshi I, Isobe T (1988) S100β positive T cell leukemia. Blood 71:1299–1303
Tanio M, Koide O, Shimazu A, Suzuki K, Yoshimatsu H (1989) Immunohistochemical studies on non-epithelial cells in thymoma tissues using paraffin-embedded sections with special reference to myastenia gravis (in Japanese). J Jpn Soc RES 29:359–365
Targan S, Brieva J, Newman W, Stevens R (1985) Is the NK lytic process involved in the mechanism of NK suppression of antibody producing cells? J Immunol 134:666–669
Trenn G, Takayama H, Sitkovsky MV (1987) Exocytosis of cytolytic granules may not be required for target cell lysis by cytotoxic T-lymphocytes. Nature 330:72–74
Ueda T, Yamaki T, Kikuchi K (1986) Functional analysis of lymphocytes infiltrating into tumors (in Japanese). Clin Immunol 18:92–102
Young DE, Hengartner JH, Podack ER, Cohn ZA (1986) Purification and characterization of cytolytic PFP from granules of cloned lymphocytes with natural killer activity. Cell 44:849–859
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Takahashi, K., Yoshino, T., Akagi, T. et al. Natural killer (NK) activity of cultured S100β-positive T-leukemia cells. Virchows Archiv B Cell Pathol 59, 159–164 (1990). https://doi.org/10.1007/BF02899400
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02899400