Summary
The isolation of a liver peptide that inhibits the growth, mitosis rate and thymidine incorporation in regenerating liver is described. The peptide has the structure Pyroglu-gln-gly-ser-asn, and the deamidated forms are also active. The peptide probably belongs to a class of growth inhibitors with a high degree of tissue specificity. Two such peptides have previously been isolated from the epidermis (Reichelt et al. 1987) and from colonic tissue (Skraastad et al. 1987).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Auger G, Blanot D, Heijenoort J van, Nadal C, Gournay M-F, Winchenne J-J, Boffa GA, Lambin P, Maes P, Tartar A (1989) Purification and partial characterization of a hepatic antip roliferative glycopeptide. J Cellular Biochem 400:39–451
Buhlen P, Castillo F, Ling R, Guillemin R (1980) Purification of peptides: an efficient procedure for the separation of peptides from amino acids and salts. Int J Peptide Prot Res 16:306–310
Burhol PG, Jenssen TG, Florhplmen J, Jorde R, Vonen B, Olsen RL (1986) Protein-binding and aggregation of somatostatin in human plasma. Ital J Gastroenterol 18:1–6
Claussen OPF, Elgjo K, Nome OD, Lærum OD, Reichelt KL, Paukowits W, Iversen OH (1986) Tissue specificity of peptide growth factors. 9th Int Chalone Conf, Milano Abs 29, p 47
Iversen OH (1981) The chalones. In: Baserga R (ed) Handbook of experimental pharmacology, vol 57. Springer, Berlin, pp 491–550
Iversen OH, Elgjo K, Reichelt KL (1989) Enhancement of methyl nitrosourea induced skin tumorigenesis and carcinogenesis by pretreatment with a mitosis-inhibiting epidermal pentapeptide Carcinogenesis 10:241–244
Elgjo K, Reichelt KL, Hennings H, Michael D, Yuspa SH (1986) Purified epidermal pentapeptide inhibits proliferation and enhances terminal differentiation in cultured mouse epidermal cells. J Invest Dermat 87:555–558
Elgjo K, Reichelt KL (1984) Purification and characterization of a mitosis inhibiting epidermal peptide. Cell Biol Int Rep 8:379–382
Elgjo K, Reichelt KL, Edminson PD, Moen E (1986) Endogenous peptides in epidermal growth control. In: Biological regulation of cell proliferation. Plenum Press, New York, pp 260–265
Elgjo K, Reichelt KL (1988) Structure and function of growth inhibitory epidermal pentapeptide. Ann NY Acad Sci 548:197–203
Gray WR (1967) Sequential degradation plus dansylation. In: Hirs CHW (ed) Methods in enzymology, XI. Academic Press, New York, pp 469–478
Hennings H, Elgjo K, Iversen OH (1969) Delayed inhibition of epidermal DNA synthesis after injection of an aqueous skin extract (chalone). Virchows Arch [B] 3:45–53
Hillar M, Santarelli I, Stolzmann Z, Wafeeg W, Allen S, Chan JYH, Patt LM, Houck JC, Wyborny (1987) Nuclear peptides from calf liver, large scale isolation and fractionation; control of gene expression in cell-free systems, and inhibition of growth of cells in culture. Bas Appl Histochem 31:299–313
Jensen PKA, Bolund L (1986) Changes in proliferating cell subpopulations and mitotic activity in human epidermal cultures treated with epithelial growth inhibitors. J Invest Dermatol 86:46–50
LaBella FL, Geiger JD, Glavin GB (1985) Administered peptides inhibit the degradation of endogenous peptides, the dilemma of distinguishing direct from indirect effects. Peptides 6:645–660
Lenfant M, Wdzie Czak-Bakala J, Guitiet E, Prome JC, Sotty D, Frindel E (1989) Inhibitor of hematopoietic pluripotent stem cell proliferation: Purification and determination of its structure. Proc Natl Acad Sci (USA) 86:779–782
Martin B, Svendsen J, Ottesen M (1977) Use of carboxy-peptidase Y for carboxy-terminal sequence determinations in proteins. Carlsberg Lab Res Comm 42:99–101
Martini F, Bossa F, Barra D (1985) Assay of pyroglutamyl aminopeptidase by high performance liquid chromatography and its use in peptide sequencing. Peptides 6:103–105
McLaughlin PJ, Zagon IS (1987) Modulation of human neuro blastoma transplantable into nude mice by endogenous opioid system. Life Sci 41:1465–1472
Moon TD (1988) The effect of opiates upon prostatic carcinoma cell growth. Biochem Biophys Res Comm 153:722–727
Paukovits WR, Laerum OD (1982) Isolation and synthesis of a hemoregulatory peptide. Z Zeilforsch 37c: 1297–1300
Paulsen JE, Reichelt KL, Pedersen AK (1987) Purification and characterization of a growth inhibitory hepatic peptide. Virchows Arch [B] 54:152–154
Reichelt KL, Johansen JH, Titlestad K, Edminson PD (1984) The isolation and characterization of an insulin releasing tetrapeptide from urines of patients with lipoatrophic diabetes. Biochem Biophys Res Comm 122:103–110
Reichelt KL, Edminson PD (1985) Principles in isolation of peptide factors from urines with retained biological activity, example from schizophrenia. In: Kalasz A, Ettre LS (eds) Chromatography, the state of the art. Hung Acad Sci 27:231–256
Reichelt KL, Elgjo K, Edminson PD (1987) Isolation and structure of an epidermal pentapeptide. Biochem Biophys Res Comm 46:1493–1501
Reichelt KL, Kvamme E (1973) Histamine dependent formation of N-acetyl-aspartyl peptides in mouse brain. J Neurochem 21:849–859
Skraastad Ø, Fossli T, Edminson PD, Reichelt KL (1987) Purification and characterisation of a mitosis inhibitory tripeptide from mouse intestinal extracts. Epithelia 1:107–119
Skraastad Ø, Reichelt KL (1988) An endogenous colon mitosis inhibitor and dietary calcium inhibit the increased colonie cellproliferation induced by cholic acid. Scand J Gastroenterol 23:801–807
Skraastad Ø, Reichelt KL (1989a) An endogenous colon mitosis inhibitor reduces proliferation of colon carcinoma cells (HT 29) in serum-restricted medium. Virchows Arch [B] 56:393–396
Skraastad Ø, Reichelt KL (1989b) An endogenous colon mitosis inhibitor reduces the increased cell proliferation in colonie epithelium induced by dieatry cholic acid and treatment with 1,2-dimethylhydrazine. Carcinogenesis 10:79–82
Watts FM, Reichelt KL, Elgjo K (1989) Pentapeptide inhibitor of epidermal mitosis: Production and responsiveness in cultures of normal, transformed and neoplastic human keratino cytes. Carcinogenesis 10:2249–2253
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Reichelt, KL., Paulsen, JE. & Elgjo, K. Isolation of a growth and mitosis inhibitory peptide from mouse liver. Virchows Archiv B Cell Pathol 59, 137–142 (1990). https://doi.org/10.1007/BF02899398
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02899398