Summary
Monoclonal antibodies which recognize one or only a few keratin polypeptides have been used to study the distribution of different keratins in benign and malignant breast lesions by immunocytochemical methods. Seven monoclonal antibodies which recognized either different keratin polypeptides by immunoblotting techniques, or identified different epithelial cell types in complex tissues were used. In two mastopathies and three fibroadenomas the antibody 1u5 stained luminal cells as well as myoepithelial cells. In contrast the antibodies CK7, Troma 1, CK2 and KA4 labeled only luminal cells, whereas antibody CKB1 decorated only myoepithelial cells. All 15 ductal carcinomas showed a uniform staining of tumor cells with the antibodies Troma 1, CK2, KA4 and 1u5. The antibody CK7 also stained all ductal carcinomas, but in two specimens the staining was heterogeneous. The antibody CKB1 decorated only the preexisting myoepithelial cells in 11 of 12 ductal carcinomas but in the remaining specimen the tumor cells were also strongly positive. Tumor cells in lobular carcinomas were labeled by antibodies CK7, Troma 1, CK2, KA4, but not by CKB1. The antibody CKS1 showed no staining of any of the benign and malignant breast lesions.
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Altmannsberger M, Osborn M, Hölscher A, Schauer A (1981) The distribution of keratin type intermediate filaments in human breast cancer. Virchows Arch [Cell Pathol] 37:277–284
Azzopardi JG (1979) Problems in breast pathology. WB Saunders, London Philadelphia Toronto
Bässler R (1978) Pathologie der Brustdrüse. In: Doerr W, Seifert G, Uehlinger E (eds) Spezielle pathologische Anatomie, Band 11. Springer, Berlin Heidelberg New York
Bartek J, Durban EM, Hallowes RC, TaylorPapadimitriou J (1985) A subclass of luminal epithelial cells in the human mammary gland, defined by antibodies to cytokeratins. J Cell Sci 75:17–33
Brulet P, Babinet C, Kemler R, Jacob F (1980) Monoclonal antibodies against trophoectoderm specific markers during mouse blastocyst formation. Proc Natl Acad Sci (USA) 77:4113–4117
Caselitz J, Wustrow J, Walther B, Weber K, Osborn M (1986) A monoclonal antibody that detects myoepithelial cells in exocrine glands, basal cells in other epithelial and basal and suprabasal cells in certain hyperplastic tissues. Virchows Arch [Pathol Anat] (in press)
Debus E, Weber K, Osborn M (1982) Monoclonal cytokeratin antibodies that distinguish simple from stratified squamous epithelia: characterization on human tissues. EMBO J 2:1641–1647
Debus E, Moll R, Franke WW, Weber K, Osborn M (1984) Immunohistochemical distinction of human carcinomas by cytokeratin staining typing with monoclonal antibodies. Am J Pathol 114:121–130
Dixon JM, Page DL, Anderson TJ (1985) Long term survivors after breast cancer. Br J Surg 72:445–448
Franke WW, Schmid E, Freudenstein C, Appelhans B, Osborn M, Weber K, Keenan TW (1980) Intermediatesized filaments of the prekeratin type in myoepithelial cells. J Cell Biol 84:633–654
Krepier R, Denk H, Wierch E, Schmid E, Franke WW (1981) Keratinlike proteins in normal and neoplastic cells of human and rat mammary glands as revealed by immunofluorescence microscopy. Differentiation 20:242–252
Lancet editorial (1985) Pathology of breast cancers in longterm survivors. Lancet (ii): 758–759
Lane EB (1982) Monoclonal antibodies provide specific intramolecular markers for the study of epithelial tonofilament organization. J Cell Biol 92:665–673
Moll R, Franke WW (1986) Cytochemical cell typing of metastatic tumors according to their cytoskeletal proteins. In: Lapis K, Liotta LA, Rabson AS (eds) Biochemistry and molecular genetics of cancer metastasis. Nijhoff Martinus, The Hague
Moll R, Franke WW, Schiller DL, Geiger B, Krepier R (1982) The catalogue of human cytokeratin polypeptides: patterns of expression of specific cytokeratins in normal epithelia, Tumors and cultured cells. Cell 31:11–24
Nagle RB, Lucas DO, McDaniel KM, Clark VA, Schmelzel GM (1985) New evidence linking mammary and extramammary Paget cells to a common cell phenotype. Am J Clin Pathol 83:431–438
Osborn M, Altmannsberger M, Debus E, Weber K (1984) Conventional and monoclonal antibodies to intermediate filament proteins in human tumor diagnosis. In: Cancer cells I. The transformed phenotype. Cold Spring Harbor Laboratory, pp 191–200
Von Overbeck J, Stahli C, Gudat F, Carmann H, Lautenschlager C, Dürmüller U, Takacs B, Miggiano V, Staehelin T, Heitz PU (1985) Immunohistochemical characterization of an antiepithelial monoclonal antibody (mAB 1u-5). Virchows Arch [Pathol Anat] 407:1–12
Pinkstaff CA (1980) The cytology of salivary glands. Int Rev Cytol 63:141–261
Tölle HG, Weber K, Osborn M (1986) Microinjection of monoclonal antibodies to vimentin, desmin and GFAP in cells which contain more than one IF type. Exp Cell Res 162:462–474
Tseng SCG, Jarvinen MJ, Nelson WG, Huang JW, Woodcock MJ, Sun TT (1982) Correlation of specific keratins with different types of epithelial differentiation: Monoclonal antibody studies. Cell 30:361–372
WHO (1981) Histological typing of breast tumors. 2nd ed, International Histological Classifica tion of Tumors, Geneva
Wu YL, Parker LM, Binder NE, Beckett JH, Sinard JH, Griffiths CT, Rheinwald JG (1983) The mesothelial keratins: A new family of cytoskeletal proteins identified in cultured me sothelial cells and nonkeratinizing epithelia. Cell 31:670–693
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Altmannsberger, M., Dirk, T., Droese, M. et al. Keratin polypeptide distribution in benign and malignant breast tumors: subdivision of ductal carcinomas using monoclonal antibodies. Virchows Archiv B Cell Pathol 51, 265–275 (1986). https://doi.org/10.1007/BF02899036
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DOI: https://doi.org/10.1007/BF02899036