Summary
A neoplastic epithelial cell line was established from nude mice tumors grown after transplantation of surgical specimens from a human parotid gland adenocarcinoma. This cell line, which had ultrastructure similarities to salivary intercalated duct cells, was found by immunohistochemical techniques to contain amylase, but myosin was not detected. Ultrastructurally, cells of an intermediate type between intercalated ductal and myoepithelial cells were found in the transplanted tumors. Moreover, the expression of myosin in addition to the presence of amylase was detected in the tumors. These findings indicate that some transplanted tumor cells appear to be differentiating towards myoepithelial cells.
Similar content being viewed by others
Abbreviations
- MEM:
-
minimal essential medium
- HSY:
-
a neoplastic intercalated duct cell line established from a human parotid gland adenocarcinoma
- PAP:
-
peroxidase-antiperoxidase
- PBS:
-
phosphate-buffered saline
References
Bloom W, Fawcett DW (1975) A textbook of histology, ed. 10. WB Saunders, Philadelphia, pp 593, 609, 915, 961
Erlander RA, Tandler B (1972) Ultrastructure of acinic cell carcinoma of the parotid gland. Arch Pathol 93: 130–140
Eversole LR (1971) Histogenic classification of salivary tumors. Arch Pathol 92: 433–443
Hara K, Ito M, Takeuchi J, Iijima S, Endo T, Hidaka H (1983) Distribution of S-100b protein in normal salivary glands and salivary gland tumors. Virchows Arch [Pathol Anat] 401: 237–249
Kay S, Schatzki PF (1972) Ultrastructure of acinic cell carcinoma of the parotid salivary gland. Cancer 29: 235–244
Kierzenbaum AL (1968) The ultrastructure of mixed salivary tumors. Lab Invest 18:391–396
Korsrud FR, Brandtzaeg P (1982) Characterization of epithelial elements in human major salivary glands by functional markers: Localization of amylase, lactoferrin, lysozyme, secretory component, and secretory immunoglobulins by paired immunofluorescence staining. J Histochem Cytochem 30: 657–666
Nakajima T, Watanabe S, Sato Y, Kameya T, Hirota T, Shimosato Y (1982) An immunoperoxidase study of S-100 protein distribution in normal and neoplastic tissues. Am J Surg Pathol 6: 715–727
Riva A, Riva-Testa F (1973) Fine structure of acinar cells of human parotid gland. Anat Rec 176: 149–166
Sato M, Hayashi Y, Yoshida H, Yanagawa T, Yura Y, Nitta T (1984) Search for specific markers of neoplastic epithelial duct and myoepithelial cell lines established from human salivary gland and characterization of their growth in vitro. Cancer 54:2959–2967
Shirasuna K, Sato M, Miyazaki T (1981) A neoplastic epithelial duct cell line established from an irradiated human salivary gland. Cancer 48: 745–752
Sternberger LA, Hardy PH, Cuculis JJ, Meyer HG (1970) The unlabelled antibody enzyme method of immunohistochemistry: Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-anti-horseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18: 315–333
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yanagawa, T., Hayashi, Y., Nagamine, S. et al. Generation of cells with phenotypes of both intercalated duct-type and myoepithelial cells in human parotid gland adenocarcinoma clonal cells grown in athymic nude mice. Virchows Archiv B Cell Pathol 51, 187–195 (1986). https://doi.org/10.1007/BF02899028
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02899028