Abstract
The ability ofPhanerochœte chrysosporium, Trametes versicolor, Coriolopsis polyzona, andPleurotus ostreatus growing in a nitrogen-limited mineral medium (NMM) to degrade PCBs in a commercial, Delor 106 mixture at a concentration of 0.9 ppm was compared. The respective amounts of PCBs removed from the fungal cultures within 3 weeks were 25, 50, 41, and 0%. The capacities of the individual fungal species to remove PCBs correlated to some extent with their capabilities of decolorization of NMM agar containing both Poly R-478 or Remazol Brilliant Blue R dyes. Enzyme estimations indicated that both high and relatively stable activities of Mn-dependent peroxidase, Mn-independent peroxidase, lignin peroxidase, and laccase characterized efficient PCB degraders.
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Barr D.P., Shah M.M., Grover T.A., Aust S.D.: Production of hydroxyl radical by lignin peroxidase fromPhanerochœte chrysosporium.Arch.Biochem.Biophys.298, 480–485 (1992).
Bogan B.W., Lamar R.T.: One-electron oxidation in the degradation of creosote polycyclic aromatic hydrocarbons byPhanerochöete chrysosporium.Appl.Environ.Microbiol.61, 2631–2635 (1995).
Bumpus J.A., Tien M., Wright D., Aust S.D.: Oxidation of persistent environmental pollutants by a white rot fungus.Science228, 1434–1436 (1985).
Dercová K., Baláž Š., Haluška L., Šturdík E., Vozárová K., Krupčík J., Benická E., Bielek P.: Degradation of Delor 103, a technical mixture of polychlorinated biphenyls, by selected bacteria.World J.Microbiol.Biotechnol.9, 648–652 (1993).
Dietrich D., Hickey W.J., Lamar R.: Degradation of 4,4′-dichlorobiphenyl, 3,3′,4,4′-tetrachlorobiphenyl, and 2,2′,4,4′,5,5′-hexachlorobiphenyl by the white rot fungusPhanerochœte chrysosporium.Appl.Environ.Microbiol.61, 3904–3909 (1995).
Eaton D.C.: Mineralization of polychlorinated biphenyls byPhanerochöete chrysosporium: a ligninolytic fungus.Enzyme Microb.Technol.7, 194–196 (1985).
Field J.A., DeJong E., Feijoo-Costa G., DeBont J.A.M.: Biodegradation of polycyclic aromatic hydrocarbons by new isolates of white rot fungi.Appl.Environ.Microbiol.58, 2219–2226 (1992).
Field J.A., DeJong E., Feijoo-Costa G., DeBont J.A.M.: Screening for ligninolytic fungi applicable to the biodegradation of xenobiotics.Trends Biotechnol.11, 44–49 (1993).
Glenn J.K., Gold M.H.: Decolorization of polymeric dyes by the lignin degrading basidiomycetePhanerochœte chrysosporium.Appl.Environ.Microbiol.45, 1741–1747 (1983).
Hatakka A.: Lignin-modifying enzymes from selected white-rot fungi: production and role in lignin degradation.FEMS Microbiol.Rev.13, 125–135 (1994).
Kerem Z., Hadar Y.: Chemically defined solid-state fermentation ofPleurotus ostreatus.Enzyme Microb.Technol.15, 785–790 (1993).
Kuwahara M., Glenn J.K., Morgan M.A., Gold M.H.: Separation and characterization of two extracellular H2O2-dependent oxidases from ligninolytic cultures ofPhanerochœte chrysosporium.FEBS Lett.169, 247–250 (1984).
Ollikka P., Alhonmäki K., Leppänen V.M., Glumoff T., Raijola T., Suominen I.: Decolorization of azo, triphenyl methane, heterocyclic, and polymeric dyes by lignin peroxidase isoenzymes fromPhanerochœte chrysosporium.Appl.Environ.Microbiol.59, 4010–4016 (1993).
Pasti M.B., Crawford D.L.: Relationship between the abilities of streptomycetes to decolorize three anthrone-type dyes and to degrade lignocellulose.Can.J.Microbiol.37, 902–907 (1991).
Rogalski J., Lundell T., Leonowicz A., Hatakka A.: Production of laccase, lignin peroxidase, and manganese-dependent peroxidase by various strains ofTrametes versicolor depending on culture conditions.Acta Microbiol.Polon.40, 221–234 (1991).
Šašek V., Volfová O., Erbanová P., Vyas B.R.M., Matucha M.: Degradation of PCBs by white rot fungi, methylotrophic and hydrocarbon utilizing yeasts and bacteria.Biotechnol.Lett.15, 521–526 (1993).
Thomas D.R., Carswell K.S., Georgiou G.: Mineralization of biphenyl and PCBs by the white rot fungusPhanerochœte chrysosporium.Biotechnol.Bioeng.40, 1395–1402 (1992).
Tien M., Kirk T.K.: Lignin peroxidase ofPhanerochœte chrysosporium.Methods Enzymol.161, 238–249 (1988).
Vyas B.R.M., Molitoris H.P.: Involvement of an extracellular H2O2-dependent ligninolytic activity of the white rot fungusPleurotus ostreatus in the decolorization of Remazol Brilliant Blue R.Appl.Environ.Microbiol.61, 3919–3927 (1995).
Vyas B.R.M., Šašek V., Matucha M., Bubner M.: Degradation of 3,3′,4,4′-tetrachlorobiphenyl by selected white rot fungi.Chemosphere28, 1127–1134 (1994).
Yadav J.S., Quensen J.F., Tiedje J.M., Reddy C.A.: Degradation of polychlorinated biphenyl mixtures (Aroclors 1242, 1254, and 1260) by the white rot fungusPhanerochœte chrysosporium as evidenced by congener-specific analysis.Appl.Environ.Microbiol.61, 2560–2565 (1995).
Zachař P., Novotný C., Voznáková Z., Matucha M., Tesařová E., Sýkora D., Kubátová A., Popl M., Šašek V.: Physical factors negatively affecting evaluation of long term biodegradation experiments of polychlorinated biphenyls.Chemosphere33, 2411–2421 (1996).
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The work was supported by a grant of theAcademy of Sciences of the Zech Republic no. A6301501 and a grant of theAgency of the Zech Republic no. 204/94/1190.
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Novotný, Č., Vyas, B.R.M., Erbanová, P. et al. Removal of PCBs by various white rot fungi in liquid cultures. Folia Microbiol 42, 136–140 (1997). https://doi.org/10.1007/BF02898723
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DOI: https://doi.org/10.1007/BF02898723