Abstract
Sugar beet grown in pots was sprayed with N6-(m-hydroxybenzyl)adenosine, (mOH)- [9R]BAP, one of the synthetic cytokinins. Root tissue was then examined for respiration and for H+-adenosinetriphosphatase activity and both leaf and root tissue served as the object for 6-deoxy-D-glucose and 2-aminoisobutyric acid uptake estimations. Treatment with (mOH)[9R]BAP depressed the uptake of oxygen by the roots of both young and old plants by 17 – 30 % while addition of (mOH)[9R]BAP to the respiring slices decreased it by 10 – 23 %. Uptake of 6-deoxy-D-glucose was mostly diminished byin vivo spraying with the cytokinin (by up to 12 % in leaves and by up to 60 % in roots), as well as by adding it to the experimental vessel (insignificantly in the leaves but by up to 80 % in the roots). The H+-ATPase activity was stimulated bothin vivo andin vitro appreciably in young plants but not at all in plants at the end of their vegetation period.
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Acknowledgement: The work described here was supported by grant No. 501/94/0413 of the Grant Agency of the Czech Republic
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Kotyk, A., Kamínek, M., Pulkrábek, J. et al. Effect ofin vivo andin vitro application of the cytokinin N6 -(m-hydroxybenzyl)adenosine on respiration and membrane transport processes in sugar beet. Biol Plant 38, 363–368 (1996). https://doi.org/10.1007/BF02896663
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DOI: https://doi.org/10.1007/BF02896663