Summary
Changes in polyamine and nucleic acid concentrations were studied during the regeneration of mouse liver. Partial hepatectomy caused an early accumulation of putrescine. There was a rapid increase in spermidine concentration parallel to an increase in RNA concentration and concomitant with a decrease in spermine concentration. Only moderate changes in DNA concentration were found during the growth period. The results are discussed in relation to transcription and replication during the restoration of mouse liver after partial hepatectomy.
This is a preview of subscription content, log in via an institution to check access.
References
Abraham, K. A.: Studies on DNA-dependent RNA polymerase from Escherichia coli. 1. The mechanism of polyamine induced stimulation of enzyme activity. Europ. J. Biochem.5, 143–146 (1968).
Agrell, I. P. S., Heby, O.: Interaction between spermine and histones at nucleoprotein formation. Hoppe-Seylers Z. physiol. Chem.352, 39–42 (1971).
Bade, E. G., Sadnik, I. L., Pilgrim, C., Maurer, W.: Autoradiographic study of DNA-synthesis in the regenerating liver of the mouse. Exp. Cell Res.44, 676–678 (1966).
Bannai, S., Terayama, H.: Template activity of chromatins isolated from regenerating rat liver. J. Biochem. (Tokyo)66, 289–295 (1969).
Baserga, R.: Biochemistry of the cell cycle: A review. Cell Tiss. Kinet.1, 167–191 (1968).
Becker, F. F.: Cell division in normal mammalian tissues. Ann. Rev. Med.20, 243–258 (1969).
Bengmark, S.: Liver regeneration. In: Tumours of the liver, ed. by Pack, G. T. and Islami, A. H., p. 187–212. Berlin-Heidelberg-New York: Springer 1970.
Bucher, N. L. R.: Regeneration of mammalian liver. Int. Rev. Cytol.15, 245–300 (1963).
— Experimental aspects of hepatic regeneration. New Engl. J. Med.277, 686–696, 738–746 (1967).
Burton, K.: A study of the conditions and mechanisms of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J.62, 315–323 (1956).
Chaudhuri, S., Lieberman, I.: Control of ribosome synthesis in normal and regenerating liver. J. biol. Chem.243, 29–33 (1968).
Dykstra, W. G., Herbst, E. J.: Spermidine in regenerating liver: Relation to rapid synthesis of ribonucleic acid. Science149, 428–429 (1965).
Hammond, J. E., Herbst, E. J.: Analysis of polyamines by thin-layer chromatography. Analyt. Biochem.22, 474–484 (1968).
Heby, O., Agrell, I. P. S.: Observations on the affinity between polyamines and nucleic acids. Hoppe-Seylers Z. physiol. Chem.352, 29–38 (1971).
Higgins, G. M., Anderson, R. M.: Experimental pathology of the liver. Arch. Path.12, 186–202 (1931).
Jänne, J.: Studies on the biosynthetic pathway of polyamines in rat liver. Acta physiol. scand., Suppl.300, 1–71 (1967).
— Raina, A.: Stimulation of spermidine synthesis in the regenerating rat liver: Relation to increased ornithine decarboxylase activity. Acta chem. scand.22, 1349–1351 (1968).
— — Siimes, M.: Spermidine and spermine in rat tissues at different ages. Acta physiol. scand.62, 352–358 (1964).
Jaffe, J. J.: Diurnal mitotic periodicity in regenerating rat liver. Anat. Rec.120, 935–954 (1954).
Jardetzky, C. D., Barnum, C. P., Vermund, H.: Deoxyribonucleic acid and phospholipide metabolism in regenerating liver and the effect of X-radiation. J. biol. Chem.222, 421–433 (1956).
Munro, H. N., Fleck, A.: Recent developments in the measurement of nucleic acids in biological materials. Analyst91, 78–88 (1966).
Pegg, A. E., Williams-Ashman, H. G.: Stimulation of the decarboxylation of S-adenosylmethionine by putrescine in mammalian tissues. Biochem. biophys. Res. Commun.30, 76–82 (1968).
Rabes, H. M., Brändie, H.: Synthesis of RNA, protein and DNA in the liver of normal and hypophysectomized rats after partial hepatectomy. Cancer Res.29, 817–822 (1969).
Raina, A.: Studies on the determination of spermidine and spermine and their metabolism in the developing chick embryo. Acta physiol. scand.60, Suppl. 218, 1–81 (1963).
— Jänne, J.: Polyamines and the accumulation of RNA in mammalian systems. Fed. Proc.29, 1568–1574 (1970).
— — Siimes, M.: Stimulation of polyamine synthesis in relation to nucleic acids in regenerating rat liver. Biochim. biophys. Acta (Amst.)123, 197–201 (1966).
Rosenthal, S. M., Tabor, C. W.: The pharmacology of spermine and spermidine. Distribution and excretion. J. Pharmacol. exp. Ther.116, 131–138 (1956).
Russell, D., Snyder, S. H.: Amine synthesis in rapidly growing tissues: Ornithine decarboxylase activity in regenerating rat liver, chick embryo, and various tumors. Proc. nat. Acad. Sci. (Wash.)60, 1420–1427 (1968).
Schwimmer, S.: Differential effects of putrescine, cadaverine, and glyoxal-bis-(guanylhydrazone) on DNA- and nucleohistone-supported DNA synthesis. Biochim. biophys. Acta (Amst.)166, 251–254 (1968).
Shimizu, H., Kakimoto, Y., Sano, I.: Changes in concentration of polyamines in the developing mouse brain. Nature (Lond.)207, 1196–1197 (1965).
Siimes, M.: Studies on the metabolism of 1,4-14C-spermidine and 1,4-14C-spermine in the rat. Acta physiol. scand., Suppl.298, 1–66 (1967).
Stevens, L.: Evidence for the presence of spermidine and spermine in the nuclei of rat liver and calf thymus gland. Biochem. J.99, 11P (1966).
— The biochemical role of naturally occurring polyamines in nucleic acid synthesis. Biol. Rev.45, 1–27 (1970).
Tabor, H., Tabor, C. W.: Spermidine, spermine, and related amines. Pharmacol. Rev.16, 245–300 (1964).
Tsuboi, K. K., Yokoyama, H. O., Stowell, R. E., Wilson, M. E.: The chemical composition of regenerating mouse liver. Arch. Biochem.48, 275–292 (1954).
Weinbren, K., Taghizadeh, A.: The mitotic response after subtotal hepatectomy in the rat. Brit. J. exp. Path.46, 413–417 (1965).
Wilson, M. E., Stowell, R. E., Yokoyama, H. O., Tsuboi, K. K.: Cytological changes in regenerating mouse liver. Cancer. Res.13, 86–92 (1953).
Yokoyama, H. O., Wilson, M. E., Tsuboi, K. K., Stowell, R. E.: Regeneration of mouse liver after partial hepatectomy. Cancer Res.13, 80–85 (1953).
Author information
Authors and Affiliations
Additional information
This investigation was facilitated by grants from the Royal Physiographical Society.
Rights and permissions
About this article
Cite this article
Heby, O., Lewan, L. Putrescine and polyamines in relation to nucleic acids in mouse liver after partial hepatectomy. Virchows Arch. Abt. B Zellpath. 8, 58–66 (1971). https://doi.org/10.1007/BF02893515
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02893515