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The intratumoral microvessel density and expression of bFGF and nm23-Hl in colorectal cancer

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Pathology & Oncology Research

Abstract

It has previously been reported that intratumoral microvessel density (IMD), and the expression of bFGF and nm23-H1 are useful prognostic markers in colorectal cancer (CRC). In this study, a total of 100 CRCs were evaluated histopathologically, and IMD, bFGF and nm23-H1 expression were assessed by immunohistochemistry. IMD of patients increased with grade and stage, and this increase was statistically significant (p<0.05). A significantly higher incidence of high bFGF expression scores was also associated with increasing grade and stage (p<0.05). However, there was no significant difference between the grades in nm23-H1 expression (p=0.234). nm23-H1 expression occurred with lower incidence in stages C1, C2 and D than in stages B1 and B2 (p<0.05). Thus, a negative correlation was found between nm23-H1 expression and stage or lymph node metastasis (LNM) (p<0.05). IMD and bFGF expression were positively correlated with grade, stage, LNM, and lymphovascular invasion. Although positive correlation was found between IMD and bFGF, nm23-H1 expression negatively correlated with both of them. As a result, in clinical practice, increased IMD and bFGF expression and decreased nm23-H1 expression may provide valuable information in characterizing the malignant phenotype.

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References

  1. Harpaz N, Saxena R: Large intestine. In: Modern Surgical Pathology. (Eds: Weidner N, Cote RJ, Suster S, Weis LM). 1st Edition, Saunders, Philadelphia, 2003, pp 749–852

    Google Scholar 

  2. Vermeulen PB, Van den Eynden GG, Huget P, et al: Prospective study of intratumoral microvessel density, p53 expression and survival in colorectal cancer. Br J Cancer 79:316–322, 1999

    Article  PubMed  CAS  Google Scholar 

  3. Weidner N, Semple JP, Welch WR, et al: Tumor angiogenesis and metastasis — correlation in invasive breast carcinoma. N Eng J Med 324: 1–8, 1991

    CAS  Google Scholar 

  4. Graham CH, Rivers J, Kerbel RS, et al: Extent of vascularization as a prognostic indicator in thin (<0.76 mm) malignant melanomas. Am J Pathol 145: 510–514, 1994

    PubMed  CAS  Google Scholar 

  5. Smith-McCune KK, Weidner N: Demonstration and characterization of the angiogenic properties of cervical dysplasia. Cancer Res 54:800–804, 1994

    PubMed  CAS  Google Scholar 

  6. Goi T, Fujioka M, Satoh Y, et al: Angiogenesis and tumor proliferation/metastasis of human colorectal cancer cell line SW620 transfected with endocrine glands derived vascular endothelial growth factor, as a new angiogenic factor. Cancer Res 64: 1906–1910, 2004

    Article  PubMed  CAS  Google Scholar 

  7. Saad RS, Liu YL, Nathan G, et al: Endoglin (CD 105) and vascular endothelial growth factor as prognostic markers in colorectal cancer. Mod Pathol 17: 197–203, 2004

    Article  PubMed  CAS  Google Scholar 

  8. Fenjvesi A: Prognostic significance of tumor-induced angiogenesis in colorectal carcinoma. Med Pregl 56: 263–268, 2003

    Article  PubMed  Google Scholar 

  9. Aotake T, Lu C, Chiba Y, et al: Changes of angiogenesis and tumor cell apoptosis during colorectal carcinogenesis. Clin Cancer Res 5: 135–142, 1999

    PubMed  CAS  Google Scholar 

  10. Shan YS, Lee JC, Chow NH, et al: Immunohistochemical microvessel count is not a reliable prognostic predictor in colorectal carcinoma. Hepatogastroenterol 50: 1316–1320, 2003

    Google Scholar 

  11. Banner BF, Whitehause R, Baker SP, et al: Tumor angiogeuesis in stage II colorectal carcinoma. Association with survival. Am J Clin Pathol 109: 733–737, 1998

    PubMed  CAS  Google Scholar 

  12. Bossi P, Viale G, Lee AKC, et al: Angiogenesis in colorectal tumors: microvessel quantitation in adenomas and carcinomas with clinicopathological correlations. Cancer Res 55: 5049–5053, 1995

    PubMed  CAS  Google Scholar 

  13. Saito S, Tsuno N, Nagawa H, et al: Expression of platelet-derived endothelial cell growth factor correlates with good prognosis in patients with colorectal carcinoma. Cancer 88: 42–49, 2000

    Article  PubMed  CAS  Google Scholar 

  14. Leung DW, Cachianes G, Kuang WJ, et al: Vascular endothelial growth factor is a secreted angiogenic mitogen. Science 246: 1306–1309, 1989

    Article  PubMed  CAS  Google Scholar 

  15. New B, Yeoman L: Identification of basic fibroblast growth factor sensitivity and receptor and ligand expression in human colon tumor cell lines. Cell Physiol 150: 320–326, 1992

    Article  CAS  Google Scholar 

  16. Steeg PS, Bevilacqua G, Kopper L et al: Evidence for a novel gene associated with low tumor metastatic potential. J Natl Cancer Inst 80: 200–204, 1998

    Article  Google Scholar 

  17. Tannapfel A, Kockerling F, Katalinic A, et al: Expression of nm23-Hl predicts lymph node involvement in colorectal carcinoma. Dis Colon Rectum 38: 651–654, 1995

    Article  PubMed  CAS  Google Scholar 

  18. Ayhan A, Yasui W, Yokozaki H, et al: Reduced expression of nm23-Hl protein is associated with advanced tumor stage and distant metastases in human colorectal carcinomas. Virchows Arch B Cell Pathol 63: 213–218, 1993

    CAS  Google Scholar 

  19. Heide I, Theide C, Poppe K, et al: Expression and mutational analysis of nm23-Hl in liver metastases of colorectal cancer. Br J Cancer 70: 1267–1271, 1994

    PubMed  CAS  Google Scholar 

  20. Lee JC, Lin YJ, Chow NH, et al: Reappraisal of the role of nm23-Hl in colorectal cancers. J Surg Oncol 76: 58–62, 2001

    Article  PubMed  CAS  Google Scholar 

  21. Garinis G A, Manolis EN, Spanakis NE, et al: High frequency of concomitant nm23-Hl and E-cadherin transcriptional inactivation in primary non-inheriting colorectal carcinomas. J Mol Med 81: 256–263, 2003

    PubMed  CAS  Google Scholar 

  22. George ML, Dzik-Jurasz ASK, Padhani AR, et al: Non-invasive methods of assessing angiogenesis and their value in predicting response to treatment in colorectal cancer. Br J Surg 88: 1628–1636, 2001

    Article  PubMed  CAS  Google Scholar 

  23. Lackner C, Jukic Z, Tsybrovskyy O, et al.: Prognostic relevance of tumor-associated macrophages and von Willebrand factorpositive microvessels in colorectal cancer. Virchows Arch 445:160–167, 2004

    Article  PubMed  CAS  Google Scholar 

  24. Hawighorts H, Knapstein PG, Knapp M.V, et al: Uterine cervical carcinoma: Comparison of standard and pharmacokinetic analysis of time intensity curves for assessment of tumor angiogenesis and patient survival. Cancer Res 58: 3598–3602, 1998

    Google Scholar 

  25. Tomisaki S, Ohno S, Ichiyoshi Y, et al: Microvessel. quantification and its possible relation with liver metastasis in colorectal cancer. Cancer 77: 1722–1728, 1996

    PubMed  CAS  Google Scholar 

  26. Tanigawa N, Amaya H, Matsumara M, et al: Tumor angiogenesis and mode of metastasis in patients with colorectal cancer. Cancer Res 57:1043–1046, 1997

    PubMed  CAS  Google Scholar 

  27. Lindmark G, Gerdin B, Sundberg C, et al: Prognostic significance of the microvascular count in colorectal cancer. J Clin Oncol 14: 461–466, 1996

    PubMed  CAS  Google Scholar 

  28. Takahashi Y, Kitadai Y, Bucana CD, et al: Expression of vascular endothelial growth factor and its receptor, KDR, correlates with vascularity, metastasis, and proliferation of human colon cancer. Cancer Res 55: 3964–3968, 1995

    PubMed  CAS  Google Scholar 

  29. Frank RE, Saclarides TJ, Leurgans S, et al: Tumor angiogenesis as a predictor of recurrence and survival in patients with node-negative colon cancer. Ann Surg 222: 695–699, 1995

    Article  PubMed  CAS  Google Scholar 

  30. White JL, Hewett PL, Kosuge D, et al: Vascular endothelial growth factor-D expression is an independent prognostic marker for survival in colorectal carcinoma. Cancer Res 62:1669–1675, 2002

    PubMed  CAS  Google Scholar 

  31. Li Z-P, Meng Q-F, Sun C-H, et al: Tumor angiogenesis and dynamic CT in colorectal carcinoma: Radiologic-pathologic correlation. World J Gastroenterol 11: 1287–1291, 2005

    PubMed  Google Scholar 

  32. Basilica C, Moscatelli D: The FGF family of growth factors and oncogenes. Adv Cancer Res 59: 115–125, 1992

    Article  Google Scholar 

  33. Landriscina M, Cassano A, Ratio C et al: Quantitative analysis of basic fibroblast growth factor and vascular endothelial growth factor in human colorectal cancer. Br J Cancer 78: 765–770, 1998

    PubMed  CAS  Google Scholar 

  34. Zheng S, Han MY, Xiao ZX, et al: Clinical significance of vascular endothelial growth factor expression and neovascularization in colorectal carcinoma. World J Gastroenterol 9: 1227–1230, 2003

    PubMed  Google Scholar 

  35. Takahashi Y, Tucker SL, Kitadai Y, et al: Vessel counts and expression of vascular endothelial growth factor as prognostic factors in node-negative colon cancer. Arch Surg 132: 541–546, 1997

    PubMed  CAS  Google Scholar 

  36. Stahl JA, Leone A, Rosengard AM, et al: Identification of a second human nm23-Hl gene, nm23-Hl-H2. Cancer Res 51: 445–449, 1991

    PubMed  CAS  Google Scholar 

  37. Xerri L, Grob JJ, Battyani Z, et al.: nm23-Hl expression in metastasis of malignant melanoma is a predictive prognostic parameter correlated with survival. Br J Cancer 70: 1224–1228, 1994

    PubMed  CAS  Google Scholar 

  38. Yamaguchi A, Urano T, Goi T, et al: Expression of human nm23-Hl and nm23-Hl-H2 proteins in hepatocelluiar carcinoma. Cancer 73: 2280–2284, 1994

    Article  PubMed  CAS  Google Scholar 

  39. Kodera Y, Isobe KI, Yamauchi M et al: Expression of nm23-Hl RNA levels in human gastric cancer tissues. Cancer 73: 259–265, 1994

    Article  PubMed  CAS  Google Scholar 

  40. Zou M, Shi Y, al-Sedairy S, et al: High levels of nm23-H1 gene expression in advanced stage of thyroid carcinomas. Br J Cancer 68: 385–388, 1993

    PubMed  CAS  Google Scholar 

  41. Engel M, Theisinger B, Seib T, et al: High levels of nm23-H1 and nm23-H1-H2 messenger RNA in human squamous-cell lung carcinoma are associated with poor differentiation and advanced tumor stages. Int J Cancer 55: 375–379, 1993

    Article  PubMed  CAS  Google Scholar 

  42. Zeng ZS, Hsu S, Zhang ZF, et al: High level of nm23-Hl gene expression is associated with local colorectal cancer progression not with metastasis. Br J Cancer 70: 1025–1030, 1994

    PubMed  CAS  Google Scholar 

  43. Yamaguchi A, Urana T, Fushida S, et al: Inverse association of nm23-H1 expression by colorectal cancer with liver metastasis. Cancer 68: 1020–1024, 1993

    CAS  Google Scholar 

  44. Lindmark G: nm23-Hl immunohistochemistry is not useful as predictor of metastatic potential of colorectal cancer. Br J Cancer 74: 1413–1418, 1996

    PubMed  CAS  Google Scholar 

  45. Sarris M, Lee CS: Nm23-Hl protein expression in colorectal carcinoma metastasis in regional lymph nodes and the liver. Eur J Surg Oncol 27: 170–174, 2001

    Article  PubMed  CAS  Google Scholar 

  46. Soliani P, Ziegler S, Romani A et al: Prognostic significance of nm23-H1 gene product expression in patients with colorectal carcinoma treated with radical intent. Oncol Rep 11: 1193–1200, 2004

    PubMed  CAS  Google Scholar 

  47. Dursun A, Akyurek N, Gunel N, et al: Prognostic implication of nm23-H1 expression in colorectal carcinomas. Pathology 34: 427–432, 2002

    Article  PubMed  CAS  Google Scholar 

  48. Dusonchet L, Corsale S, Migliavacca M et al: Nm23-Hl expression does not predict clinical survival in colorectal cancer patients. Oncol Rep 10: 1257–1263, 2003

    PubMed  CAS  Google Scholar 

  49. Royds JA, Cross SS, Silcocs PB, et al: Nm23-H1 “anti-metastatic” gene product expression in colorectal carcinoma. J Pathol 172: 261–266, 1994

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Pathology, Cumhuriyet University School of Medicine, 58140, Sivas, Turkey

    Sahande Elagoz, Reyhan Egilmez, Ahmet Muslehiddinoglu & Sema Arici

  2. Department of General Surgery, Cumhuriyet University School of Medicine, Sivas, Turkey

    Ayhan Koyuncu

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  1. Sahande Elagoz
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  2. Reyhan Egilmez
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  3. Ayhan Koyuncu
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  4. Ahmet Muslehiddinoglu
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  5. Sema Arici
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Correspondence to Sahande Elagoz.

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Elagoz, S., Egilmez, R., Koyuncu, A. et al. The intratumoral microvessel density and expression of bFGF and nm23-Hl in colorectal cancer. Pathol. Oncol. Res. 12, 21–27 (2006). https://doi.org/10.1007/BF02893427

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  • DOI: https://doi.org/10.1007/BF02893427

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