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Immunohistological demonstration of the same type of pyruvate kinase isoenzyme (M2-Pk) in tumors of chicken and rat

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Virchows Archiv B

Summary

Tumors of chicken and rat contain the M2-type of pyruvate kinase isoenzyme. The amount of this isoenzyme is significantly higher in malignant tumors compared with benign tumors. No alteration of the normal pyruvate kinase isoenzyme pattern was found in hyperplastic tissue.

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References

  • Balinsky D, Cayanis E, Geddes EW, Bersohn I (1973) Activities and isoenzyme patterns of some enzymes of glucose metabolism in human primary malignant hepatoma. Cancer Res 33: 249–255

    PubMed  CAS  Google Scholar 

  • Boxer GE, Shank CE (1966) Comparison of glycolytic enzyme activities in a series of human and rodent hepatomas. Adv Enzyme Regul 4: 107–114

    Article  PubMed  CAS  Google Scholar 

  • Cardenas JM, Blachly EG, Cecotti PL, Dyson RD (1975) Properties of chicken skeletal muscle pyruvate kinase and a proposal for its evolutionary relationship to the other avian and mamalian isozymes. Biochemistry 14: 2247–2252

    Article  PubMed  CAS  Google Scholar 

  • Criss WE (1971) A review of isoenzymes in cancer. Cancer Res 31: 1523–1542

    PubMed  CAS  Google Scholar 

  • Deng JS, Beutner EH (1974) Effect of formaldehyde, glutaraldehyde and sucrose on the tissue antigenicity. Int Arch Allergy 47: 562–569

    PubMed  CAS  Google Scholar 

  • Eigenbrodt E, Glossmann H (1980) Glycolysis one of the keys to cancer? Trends Pharmacol Sci 9: 240–245

    Article  Google Scholar 

  • Elias JH, Johnson FA (1979) The utilization of radioimmunoassay antibodies for the immunohistologic staining of polypeptide hormones on paraffin embedded tissues. Am J Clin Pathol 71: 489–491

    PubMed  CAS  Google Scholar 

  • Farina AF, Shatton JB, Morris HP, Weinhouse S (1974) Isoenzymes of pyruvate kinase in liver and hepatoma of the rat. Cancer Res 34: 1435–1446

    Google Scholar 

  • Fottrell PF, Spellman CM (1976) Immunological similarities between pyruvate kinase from human placenta and human tumours. In: Franchimont P (ed) Cancer related antigens. Elsevier/North Holland Biomédical Press, Amsterdam, pp 115–123

    Google Scholar 

  • Graham RC, Karnovsky M. J. (1966) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney. Ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14: 291–302

    PubMed  CAS  Google Scholar 

  • Hall ER, Cottam GL (1978) Isozymes of pyruvate kinase in vertebrates: Their physical, chemical, kinetic and immunological properties. Int J Biochem 9: 185–193

    Article  Google Scholar 

  • Hammond KD, Balinsky D (1978) Isozyme studies of several enzymes of carbohydrate metabolism in human adult and fetal tissues, tumor tissues and cell cultures. Cancer Res 38: 1323–1328

    PubMed  CAS  Google Scholar 

  • Imamura K, Taniuchi K, Tanaka T (1972) Multimolecular forms of pyruvate kinase. II. Purification of M2-type pyruvate kinase from Yoshida ascites hepatoma 130 cells and comparative studies on the enzymological and immunological properties of the three types of pyruvate kinases, L, M1, and M2. J Biochem 72: 1001–1015

    PubMed  CAS  Google Scholar 

  • Isobe Y, Chen S-T, Nakane PK, Brown WR (1977) Studies on translocation of immunoglobulin across intestinal epithelium. I. Improvements in the peroxidase-labeled antibody method for application to study of human intestinal mucosa. Acta Histochem Cytochem 10: 161–171

    CAS  Google Scholar 

  • Marie J, Kahn A (1977) Purification of L-type pyruvate kinase from human liver by affinity chromatography on blue-dextran. Enzyme 22: 407–411

    PubMed  CAS  Google Scholar 

  • Nakamura T, Hosoi K, Nishikawa K, Horio T (1972) Effect of injection of chromatins from rhodamine sarcoma into rats on pI-isozymes of liver pyruvate kinase. Gann 63: 239–250

    PubMed  CAS  Google Scholar 

  • Reinacher M, Eigenbrodt E, Schering B, Schoner W (1979) Immunohistochemical localization of pyruvate kinase isoenzymes in chicken tissues. Histochemistry 64: 145–161

    Article  PubMed  CAS  Google Scholar 

  • Riou JP, Claus TH, Pilkis SJ (1978) Stimulation by glucagon of in vivo phosphorylation of rat hepatic pyruvate kinase. J Biol Chem 255: 668–675

    Google Scholar 

  • Spellman CM, Fottrell PD (1973) Similarities between pyruvate kinase from human placenta and tumours. FEBS Lett 37: 281–284

    Article  PubMed  CAS  Google Scholar 

  • Stefanini M, de Martino C, Zamboni L (1967) Fixation of ejaculated spermatozoa for electron microscopy. Nature 216: 173–174

    Article  PubMed  CAS  Google Scholar 

  • Tanaka T, Harano Y, Morimura H, Mori R (1965) Evidence for the presence of two types of pyruvate kinase in rat liver. Biochem Biophys Res Commun 21: 55–60

    Article  PubMed  CAS  Google Scholar 

  • Tanaka T, Harano Y, Sue T, Mori R (1967) Crystallization, characterization and metabolic regulation of two types of pyruvate kinase isolated from rat tissues. J Biochem (Tokyo) 62: 71–91

    CAS  Google Scholar 

  • van Veelen CWM, Verbiest H, Vlug AMC, Rijksen G, Staal GEJ (1978) Isozymes of pyruvate kinase from human brain, meningiomas and malignant gliomas. Cancer Res 38: 4681–4687

    PubMed  Google Scholar 

  • van Veelen CWM, Verbiest H, Zülch KJ, van Ketel BA, van der Vlist MJM, Vlug AMC, Rijksen G, Staal GEJ (1979) L-α-alanine inhibition of pyruvate kinase from tumors of the human control nervous system. Cancer Res 39: 4263–4269

    PubMed  Google Scholar 

  • Walker PR, Potter VR (1972) Isozyme studies on adult, regenerating precancerous and developing liver in relation to findings in hepatomas. Adv Enzyme Regul 10: 339–364

    Article  PubMed  CAS  Google Scholar 

  • Weber G (1972) Molecular correlation concept: Ordered pattern of gene expression in neoplasia. Gann 13: 47–77

    Google Scholar 

  • Weinhous S (1973) Metabolism and isozyme alterations in experimental hepatomas. Fed Proc 32: 2162–2167

    Google Scholar 

  • Yanagi S, Makiura S, Arai M, Marsumura K, Hirao K, Ito N, Tanaka T (1974) Isozyme patterns of pyruvate kinase in various primary liver tumors induced during process in hepatocarcinogenesis. Cancer Res 34: 2283–2289

    PubMed  CAS  Google Scholar 

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Supported by the “Deutsche Forschungsgemeinschaft” (Re 532/1-1)

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Reinacher, M., Eigenbrodt, E. Immunohistological demonstration of the same type of pyruvate kinase isoenzyme (M2-Pk) in tumors of chicken and rat. Virchows Archiv B Cell Pathol 37, 79–88 (1981). https://doi.org/10.1007/BF02892557

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  • DOI: https://doi.org/10.1007/BF02892557

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