Skip to main content
Log in

Preparation of Chloroplast DNA from Barley and Lettuce and Comparison of Restriction Fragments

  • Published:
Biologia Plantarum

Abstract

Chloroplast DNA from three barley cultivars and from one lettuce cultivar was prepared from chloroplasts isolated by Conventional differential centrifugation. Barley chloroplast DNA size was sensibly lower (130 kpb) than lettuce chloroplast DNA (150 kpb). Chloroplast DNAs from the three barley cultivars showed similar restriction fragment patterns after digestion with: BamHI, EcoRI or HindIII. The lettuce chloroplast DNA restriction pattern was very different from the barley chloroplast DNA restriction pattern.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Callb, F., Martin, M., Sabater, B.: Cytoplasmic and mitocliondrial localization of the glutamate dehydrogenase induced by senescence in barley (Hordeum vulgäre). - Physiol. Plant.66: 451–456,1986.

    Article  Google Scholar 

  • Clbgg, M. T., BroWn, A. H. D., Whitfeld, P. R.: Cliloroplast DNA diversity in wild and cultivated barley: implications for genetic conservation. - Genet. Res.13: 339–343, 1084.

    Google Scholar 

  • Cukllo, J., Sabater, B.: Control of some enzymes of nitrogen metabolism during senescence of detached barley (Hordeum vulgäre L.) leaves. - Plant Cell Physiol.23: 561–565, 1982.

    Google Scholar 

  • Garcia, S., Martin, M., Sabater, B.: Protein synthesis by chloroplasts during the senescence of barley leaves. - Physiol. Plant.57: 260–266, 1983.

    Article  CAS  Google Scholar 

  • Hermann, R. G.: The preparation of circular DNA fromplastids. - In:Edelman, M., Hallicf, R. B., Chita, N. H. (ed.): Methods in Cliloroplast Molecular Biology. Pp. 259–280. Elsevier Biomedical, Amsterdam 1982.

    Google Scholar 

  • Hirai, A., Ishibashi, T., Morikami, A., Iwatsuki, N., ShinozakI, K., SuGuvra, M.: Rice chloroplast DNA: a physical map and the localization of the genes for the large subunit of ribulose- 1, 5-bisphosphate carboxylase and the 32 KD photosystetn II reaction center protein. - Theor. appl. Genet.70: 117–122, 1985.

    CAS  Google Scholar 

  • Holwerda, B., Jana, S., Crosby, W.: Chloroplast and mitocliondrial DNA variation in Hordeum vulgare andHordeum spontaneum, - Genetics114: 1271–1291, 1986.

    PubMed  CAS  Google Scholar 

  • Jansen, R. K., Palmer. J. D.: Chloroplast DNA from lettuce and Barnadesw (Asteraceac): structure, gene localization, and characterization of a large inversion. - Curr. Genet.11: 553–564, 1987.

    Article  CAS  Google Scholar 

  • Maniatis, T., Fritsch, E. F., Sambrook, J.: Molecular Cloning: a Laboratory Manual. - Cold Spring Harbor Laboratory, Cold Spring Harbor. New York 1982.

    Google Scholar 

  • Martin, M., Urteaga, B., Sabater, B.: Chloroplast protein synthesis during barley leaf growth and senescence: effect of leaf excision. - J. exp. Bot.37: 230–237, 1986.

    Article  CAS  Google Scholar 

  • Palmer, J. D.: Chloroplast DNA and molecular phylogeny. - BioEssays2: 263–267, 1985.

    Article  CAS  Google Scholar 

  • Poulsen, B.: The barley chloroplast genome: physical structure and transcriptional activity in vivo. - Carlsberg Res. Commun.48: 57–80, 1983.

    Article  CAS  Google Scholar 

  • Sabater, B.: Hormonal regulation of senescence. - In: Purohit, S. S. (ed.): Hormonal Regulation of Plant Growth and Development. Pp. 169–217. M. Nijhoff/Dr. W. Junk Publ., Dordrecht, 1985.

    Google Scholar 

  • Yu, S. M., Kao, C. H.: Retardation of leaf senescence by inhibitors of RNA and protein synthesis. - Physiol. Plant.52: 207–210, 1981.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Martin, M., Tomás, R., Cruz Izquierdo, M. et al. Preparation of Chloroplast DNA from Barley and Lettuce and Comparison of Restriction Fragments. Biol Plant 31, 67–71 (1989). https://doi.org/10.1007/BF02890680

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02890680

Additional index words

Navigation