Summary
In continuation of a previous study, the quantitative fine structural characteristics of the average normal human pan-T lymphocyte and its subsets in the peripheral blood has been established using stereological methods. T cell subpopulations were isolated and identified by means of monoclonal T3, T4 and T8 antibodies and a fluorescence-activated cell sorting machine. Comparative studies on ultrastructural morphology were made between the E-rosetting lymphocyte and the T3-positive cell, both markers of the pan-T cell, and between the functionally different T4- and T8-reactive subsets, defining the helper/inducer and suppressor/cytotoxic T cell subpopulation respectively.
No significant differences were recorded between the E-rosetting and the T3+ lymphocyte except for minor deviations regarding the surface of the plasma membrane. In comparison with the T4+ lymphocyte, the T8+ cell showed a larger cell volume and cell surface area and decreased nucleo/cytoplasmic volume and surface ratios. The increase in cell size was the result of greater volumes of residual cytoplasm as well as of intracytoplasmic organelles, such as mitochondria, RER, Golgi apparatus and granules, whereas nuclear parameter estimates were concordant. The structural deviations between the T4+ and the T8+ subsets are discussed in the context of their different functional capacity as helper and suppressor/cytotoxic cells.
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Al-Hamdani MM, Atkinson ME, Mayhew TM (1981) Ultrastructural morphometry of blasto-genesis. II. Stimulated lymphocytes, the progeny of blast cells induced in vivo with DNCB. Cell Tiss Res 215:643–649
Boesen AM, Hokland P (1982) Stereological analysis of the ultrastructure in isolated human T and non-T lymphoid cells. I. Description of method and data on normal blood lymphocytes. Virchows Arch [Cell Pathol] 39:273–284
Bøyum A (1968) Separation of leucocytes from blood and bone marrow. Scand J Clin Lab Invest (Suppl) 21:97
Darzynkiewicz Z, Andreeff M, Traganes F, Sharpless T, Melamed MR (1978) Discrimination of cycling and non-cycling lymphocytes by BUdR-suppressed acridine orange fluorescence in a flow cytometric system. Exp Cell Res 115:31–35
Ferrarini M, Cardoni A, Franzi AT, Ghigliotti C, Leprini A, Zicca A, Grossi CE (1980) Ultrastructure and cytochemistry of human peripheral blood lymphocytes. Similarities between the cells of the third population and TG lymphocytes. Eur J Immunol 10:562–570
Grossi CE, Greaves MF (1981) Atlas of blood cells. Function and pathology, vol 2. Part 1. Normal lymphocytes. In: Zucker-Franklin D, Greaves MF, Grossi CE, Marmont AM (eds) Edi Ermes, Milano, pp 347–408
Grossi CE, Webb SR, Zicca A, Lydyard PM, Moretta L, Mingari MC, Cooper MD (1978) Morphological and histochemical analysis of two human T-cell subpopulations bearing receptors for IgM or IgG. J Exp Med 147:1405–1417
Grossi CE, Cadoni A, Zicca A, Leprini A, Ferrarini M (1982) Large granular lymphocytes in human peripheral blood: ultrastructural and cytochemical characterization of the granules. Blood 59:277–283
Koehler G, Milstein C (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256:971–978
Mayhew TM, White FH (1980) Ultrastructural morphometry of isolated cells: Methods, models and applications. Pathol Res Pract 166:239–259
Moretta L, Ferrarini M, Cooper MD (1978) Characterization of human T-cell subpopulations as defined by specific receptors for immunoglobulins. Contemp Top Immunobiol 8:19–24
Petrzilka GE, Schroeder HE (1979) Activation of human T-lymphocytes. A kinetic and stereological study. Cell Tiss Res 201:101–127
Reinherz EL, Schlossman SF (1980) Current concepts in immunology. Regulation of the immune response — inducer and suppressor T-lymphocyte subsets in human beings. N Engl J Med 303:370–373
Reinherz EL, Moretta L, Roper M, Breard M, Mingari MC, Cooper MD, Schlossman SF (1980) Human T lymphocyte subpopulations defined by Fc receptors and monoclonal antibodies. A comparison. J Exp Med 151:969–974
Renau-Piqueras J, Cervera J (1979) Alterations of cell surface morphology in human T-rosetting lymphocytes. An ultrastructural study. Acta Haemat 62:185–190
Weibel ER (1969) Stereological principles for morphometry in electron microscopic cytology. Int Rev Cytol 26:235–302
Weibel ER (1980) Stereological methods, vol. 1. Practical methods for biological morphometry. Academic Press, London
Zicca A, Leprini A, Cadoni A, Franzi AT, Ferrarini M, Grossi CE (1981) Ultrastructural localization of alpha-naphthyl acid esterase in humanT M lymphocytes. Am J Pathol 105:40–46
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Boesen, A.M., Hokland, P. Ultrastructure of normal human blood T lymphocyte subsets isolated by cell sorting using monoclonal antibodies. Virchows Archiv B Cell Pathol 41, 107–117 (1982). https://doi.org/10.1007/BF02890275
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DOI: https://doi.org/10.1007/BF02890275