Summary
Alterations in the nuclear and cellular size of human hepatocytes occurring with age, and particularly in senescence, were studied by microphotometry. The material studied was obtained in 35 cases of sudden death, involving 17 males and 18 females ranging in age from 16 to 100 years. Cells of the peripheral zones of hepatic lobules were analyzed.
The following results were obtained:
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1.
The mean nuclear area of hepatocytes remained relatively constant in subjects under 60 years of age but showed an increase in those over 60, this increase being associated with a greater standard deviation.
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2.
Volumetric analysis showed that the modal value included between 61 and 100% (mean 86%) of the cell nuclei examined and did not increase with age. This cell population was presumed to consist of diploid cells, the size of which remained constant.
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3.
An increase in mean nuclear area was due to the appearance of cells with larger nuclei which probably were the result of polyploidization.
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4.
Hepatocyte size increased with age. Analysis of the nucleus-to-cell sizes showed that the increase in cell size with age was more significant than the increase in nuclear size.
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5.
Cellular enlargement was more closely correlated with decrease in gross liver weight than with nuclear enlargement.
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References
Altmann, H.W., Loeschke, K., Schenck, K.: Über das Karyogramm der menschlichen Leber unter normalen und pathologischen Bedingungen. Virchows Arch. path. Anat.341, 85–101 (1966)
Andrew, W., Brown, H.M., Johnson, J.B.: Senile changes in the liver of mouse and man, with special reference to the similarity of the nuclear alterations. Amer. J. Anat.72, 199–221 (1943)
Clara, M.: Untersuchungen an der menschlichen Leber. II. Über die Kerngrößen in den Leberzellen, zugleich über Amitose und über das Wachstum der “stabilen Elemente”. Ztschr. mikr. anat. Forsch.22, 145–219 (1930)
Fautrez, J., Laquerriere, R.: Teneur en acide désoxyribonucléique et volume des noyaux des cellules hépatiques chez l’homme. Exptl. Cell Res.13, 403–405 (1957)
Jacobj, W.: Volumetrische Untersuchungen an dem Zellkern des Menschen und das allgemeine Problem der Zellkerngröße. Anat. Anz. (Suppl.)72, 236–247 (1931)
Ross, M.H.: Aging, nutrition and hepatic enzyme activity patterns in the rat. J. Nutrition97, 563–602 (1969)
Shima, A., Sugahara, T.: Age dependent ploidy class changes in mouse hepatocyte nuclei as revealed by Feulgen-DNA cytofluorometry. Exptl. Geront.11, 193–203 (1976)
Swartz, F.J.: The development in the human liver of multiple desoxyribose nucleic acid (DNA) classes and their relation to the age of the individual. Chromosoma8, 53–72 (1956)
Tauchi, H., Sato, T.: Some micromeasuring studies on hepatic cells in senility. J. Geront.17, 254–259(1962)
Van Bezooijen, C.F.A., van Noorde, M.J., Knooke, D.L.: The viability of parenchymal liver cells isolated from young and old rats. Mech. Ageing & Develop.3, 107–119 (1974)
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Watanabe, T., Shimada, H. & Tanaka, Y. Human hepatocytes and aging: A cytophotometrical analysis in 35 sudden-death cases. Virchows Arch. B Cell Path. 27, 307–316 (1978). https://doi.org/10.1007/BF02889003
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DOI: https://doi.org/10.1007/BF02889003