Abstract
A 7.3 kb BamH I genomic fragment from fowlpox virus (FPV) strain 282E4 has been sequenced. After comparison with the FPV sequences collected in Genbank, and the entire genomic sequence of vaccinia virus (VV) strain Compenhagen, a ll.2 kb BamH I fragment from FPV strain Munich HP-438 has been found homologous to the fragment. Every predicted open reading frame (ORF) within the fragment has been compared with the ORFs of VV strain Copenhagen at the amino acid level. The signal peptide and transmembrane region of each peptide encoded by each corresponding ORF predicted in the fragment have been studied as well.
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Boyle, D. B., Coupar, B. E. H., Gibbs, A. J.et al., Fowlpox virus thymidine kinase: nucleotide sequence and relationships with other thymidine kinases,Virology, 1987, 156: 355.
Binns, M. M., Tomley, F. M., Camphell, J.et al., Comparison of a conserved region in fowlpox virus and vaccinia virus genomes and the translocation of the fowlpox virus thymidine kinase gene,J. Gen Virol., 1988, 69: 1275.
Gu, W. J., Jin, N. Y., Yin, Z., Sequence analysis of TK gene in 2.2 kb fragment of fowlpox virus 282E4 genome,Chinese Journal of Veterinay Science (in Chinese), 1995, 15(1): 43.
Binns, M. M., Stenzler, L., Tomley, F. M.et al., Identification by a random sequencing strategy of the fowlpox virus DNA polymerase gene, its nucleotide sequence and comparison with other viral DNA polymerase,Nucleic Acids Reserch, 1981, 15: 6563.
Tomley, F., Binns, M., Campbell, J.et al., Sequence analysis of an 11.2 kilobase, near-terminal,Bam H I fragment of fowlpox virus,J. Gen Virol., 1988, 69: 1025.
Tartaglia, J., Winslow, J., Goebel, D.et al., Nucleotide sequence analysis of a 10.5 khpHind III fragment of fowlpox virus, relatedness to the central portion of the vaccinia virusHind III D region,J. Virol., 1990, 71: 1517.
Campbell, J. I. A., Binns, M. M., Tomley, F. M.et al., Tandem repeated sequences within the terminal region of the fowlpox virus genome,J. Gen. Virol., 1989, 70: 145.
Kumar, S., Boyle, D. B., Mapping of a major early/late gene of fowlpox virus,Virus Res., 1990, 15: 175.
Calvert, J. G., Ogawa, R., Yanagida, N.et al., Identification and functional analysis of the fowlpox virus homolog of the vaccinia virus p37k major envelope antigen gene,Virology, 1992, 191: 783.
Ogawa, R., Calvert, J. G., Yanagida, N.et al., Insertional inactivation of a fowlpox virus homologue of the vaccinia virus F121, gene inhibits the release of enveloped virions,J. Gen. Virol., 1993, 74: 55.
Binns, M. M., Bousnell, M. E. G., Tomley, F. M.et al., Analysis of the fowlpox virus gene encoding the 4b core polypeptide and demonstration that it possesses efficient promoter sequences,Virology, 1989, 170: 288.
Zantinge, J. L., Krell, P. J., Derbyshire, J. B.et al., Partial transcriptional mapping of the fowlpox virus genome and analysis of the EcoR I L fragment,J. Gen. Virol., 1996, 77: 603.
Binns, M. M., Brittan, B. S., Masan, C.et al., Analysis of the fowlpox virus genome region corresponding to the vaccinia virus D6 to A1: location of, and variation in non-essential genes in poxviruses,J. Gen. Virol., 1990, 71: 2873.
Gu, W. J., Wang, S. M., Jin, Y.et al., The cloning and analysis ofBam H I fragments of PPV 282E4 genome,Journal of Jilin Agricultural University (in Chinese), 1995, 17(3): 1.
Guo, W. J., Jin, N. Y., Zhou, N. C.et al., Enzymogram analysis and identification of gene in 7.3 kbBam H I non-essential fragment of fowlpox virus 282E4 genome,Chinese Journal of Veterinary Science (in Chinese), 1995, 15(2): 193.
Si, X. K., Gu, W. J., Jin, N. Y.et al., Subcloning ofBam H I 7.3 kb fragment of FPV 282E4 strain,Journal of Jilin Agricultural University (in Chinese), 1996, 18(2): 61.
Sambrook, F., Fritsch, E. F., Maniatis, T.,Molecular Cloning: A Laboratory Mannul, 2nd ed., New York: Cold Spring Harbor Laboratory Press, 1989.
Scott, J. G., Gerard, P. J., Marion, E. P.,et al., The complete DNA sequence of vaccinia virus,Virol., 1990, 179: 247.
Nazerian, K. Structual analysis of unstable intermediate and stable forms of recomdinant fowlpox virus,J. Gen. Virus., 1991, 72: 2791.
Fatkunmbi, O. O., Read, W. M., Evaluation of commercial modified live fowlpox vaccine for the control of “variant” fowlpox virus infectious,Avian. Dis., 1996, 40(3): 582.
Mishra, S. S., Mallick, B. B., Comparative immunological and genomic characterization of fowlpox virus isolates,Indian J. Exp., 1996, 34(1): 11.
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Jin, N., Guo, Z., Luo, K. et al. Sequence analysis of a 7.3 kbBam H I genomic fragment of fowlpox virus strain 282E4 . Chin. Sci. Bull. 44, 2068–2072 (1999). https://doi.org/10.1007/BF02884923
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DOI: https://doi.org/10.1007/BF02884923