Abstract
The dynamic of degradation of antigen (human serum albumin) by rabbit cathepsin D was studied in the presence of homologous immunoglobulin G having antibody activity to substrate (antigen) as well as without antibody activity. Both immunoglobulins possessed an inhibitory effect on the intensity of antigen degradation, however, immunoglobulin G with antibody activity had a more pronounced effect. The studies on the kinetics of inhibition showed that we were probably dealing with a special case of inhibition of the mixed type which is known as coupling or anticompetitive inhibition.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ballieux, R. E.:Structuranalyse en Classificatie van π 2A -paraproteinin. Schotanus and Jens, Utrecht 3 1963.
Baumstark, J. S., Laffin, R. J., Bardwill, W. A.:A preparative method for the separation of 7S gamma globulin from human serum. Arch. Biochem. Biophys. 108: 514, 1964.
Dodgson, K. S., Spencer, R., Williams, K.:Examples of anticompetitive inhibition. Nature 177: 432, 1956.
Friedenwald, J. S., Maengwyn-Davies, G. D.:The mechanism of enzyme action (Eds. McElroy, W. D. & Glass, B.). John Hopkins Press, Baltimore 1954.
Grabar, P.:The hypothesis of “Carrier-globulins”. In: Molecular and Cellular Basis of Antibody Formation, p. 621. Publ. house Czechoslov. Acad. Sci., Prague, 1965.
Heidelberger, M., Kendall, F. E.:A quantitative study of the precipitin reaction between type III Pneumococcus polysaccharide and purified homologous antibody. J. exptl. Med. 50: 809, 1929.
Heremans, J. F., Heremans, M. T.:Studies on “abnormal” serum globulins (M-components) in myeloma, macroglobulinemia and related diseases. Acta Med. Scand. 367 (Supl.): 27, 1961.
Lapresle, C.:Hétérogénicité des anticorps antiprotéines. Ann. Inst. Pasteur 97: 626, 1959.
Lapresle, C., Kaminski, M., Tanner, C. E.:Immunochemical study of the enzymatic degradation of human serum albumin. An analysis of the antigenic structure of a protein molecule. J. Immunol. 82: 94, 1959.
Lapresle, C., Webb, T.:Study of proteolytic enzyme from rabbit spleen. Biochem. J. 76: 538, 1960a.
Lapresle, C., Webb, T.:Étude de la dégradation de la sérumalbumine humaine par un extrait de rate de lapin. Ann. Inst. Pasteur. 99: 523, 1960b.
Lapresle, C., Webb, T.:The purification and properties of a proteolytic enzyme, rabbit cathepsin E and further studies on rabbit cathepsin D. Biochem. J. 84: 455, 1962.
Libenson, L., Jena, M.:Role of the glutathione in the catheptic hydrolysis of plasma albumine. Arch. Biochem. Biophys. 104: 292, 1964.
Mannik, M.:Binding of albumin to γ-A-myeloma proteins and Waldström macroglobulins by disulfide bonds. J. Immunol. 99: 899, 1967.
McDuffic, F. C., Kabat, E. A.:A comparative study of methods used for analysis of specific precipitates in quantitative immunochemistry. J. Immunol. 77: 193, 1956.
Štefanovič, J.:Proteases of rabbit polymorphonuclear leukocytes, macrophages and lymphocytes. and their relation to the degradation of antigen. (In Slovak) Folia Fac. Med. Univ. Comenianae 2: 3, 1964.
Štefanovič, J., Webb, T., Lapresle, C.:Étude des cathepsines D et E dans les préparations de polynucléaires, de macrophages et de la lymphocyte du lapin. Ann. Inst. Pasteur. 108: 108, 1962.
Uriel, J.:A method for the direct detection of proteolytic enzymes after electrophoresis in agar gel. Nature 188: 853, 1961.
Webb, J. L.:Enzyme and metabolic inhibitors I. Acad. Press, N. Y.-London 1963.
Weiglo, W. O.:Secondary and non-specific anamnestic response. In: Mechanism of Antibody Formation, p. 283. Publ. house Czechoslov-Acad. Sci., Prague, 1960.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Štefanovič, J., Ferenčík, M. The inhibitory effect of homologous immunoglobulin G on the degradation of antigen (human serum albumin) by rabbit cathepsin D. Folia Microbiol 14, 571–577 (1969). https://doi.org/10.1007/BF02884171
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02884171