Skip to main content
Log in

Effects of extracellular calmodulin on pollen germination and tube growth

  • Bulletin
  • Published:
Chinese Science Bulletin

Abstract

The effects of calmoddin (CaM) antagonist W7-agarose, anti-CaM serum and exogenous purified CaM on pollen germination and tube growth ofForsythia suspensu were studied. The pollen germination and tube growth were inhibited or completely stopped by CaM antagonist W7-agarose. The addition of exogenous purified CaM stimulated pollen germination and tube growth, whereas the same amount of bovine serum albumin (BSA) had no effect. The inhibitory effects caused by W7-agarose and anti-CaM serum could be reversed completely by the addition of exogenous purified CaM. The tube growth of germinated pollen was also inhibited or completely stopped by W7-agarose. The results suggest that endogenous extracellular CaM initiates pollen germination and tube growth, whereas exogenous CaM enhances the above processes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Sun, D. Y., Tang, J., Li, H. B., The studies on the existence and significance of extracellular calmodulin,Chinese Science Bulletin (in Chinese), 1995, 40(13): 1153.

    Google Scholar 

  2. Biro, R. L., Sun, D. Y., Serlin, B. S.et al., Characterization of oat calmodulin and radioimmunoassay of its subcellular distribution,Plant Physiology, 1984, 75(2): 382.

    Article  PubMed  CAS  Google Scholar 

  3. Ye, Z. H., Sun, D. Y., Guo, J. F., Preliminary study on wheat cell wall calmodulin,Chinese Science Bulletin (in Chinese), 1988, 33(8): 624.

    Google Scholar 

  4. Li, J. X., Liu, J. W., Sun, D. Y., Immunoelectron microscopic localization of calmodulin in corn root cells,Cell Research, 1993, 3(1): 11.

    Google Scholar 

  5. Li, H. B., Cheng, G., Sun, D. Y., The effect of extracellular calmodulin on proliferation of suspension culture cells ofAngelica duhuric, Chinese Science Bulletin, 1992, 38: 1028.

    Google Scholar 

  6. Sun, D. Y., Li, H. B., Cheng, G., Extracellular calmodulin accelerates the proliferation of suspension-cultured cells ofAngelica dahurica, Plant Science, 1994. 99(1): 1.

    Article  CAS  Google Scholar 

  7. Sun, D. Y., Hian, Y. Q., Zhao, B. H.et al., The effects of extracellular calmodulin on cell wall regeneration of protoplasts and cell division,Pant Cell Physiology, 1995, 36(1): 133.

    CAS  Google Scholar 

  8. Tang, J., Wu, S. P., Sun, D. Y., Extracellular calmodulin-binding proteins in plants: purification of a 21-kDa calrnodulin binding protein,Plunta, 1996, 198(3): 510.

    Google Scholar 

  9. Polito, V. S., Calmodulin and calmodulin inhibitors: effects on pollen germination and tube growth, inPollen: Biology and Implications for Plant Breeding (eds. Mulcahy, D. L., Ottaviano, E.), New York: Elsevier Publishing Co. Inc., 1983, 53–60.

    Google Scholar 

  10. Gong, M., Yang, Z. H., Cao, Z. X., Involvement of calmodulin in pollen germination and pollen tube growth,Acta Phytophysiologica Sinicu, 1994, 20(3): 240.

    CAS  Google Scholar 

  11. Malho, R., Read, N. D., Trewavas, A. J.et al., Calcium channel activity during pollen tube growth and reorientation,Plant Cell, 1995, 7(8): 1173.

    Article  PubMed  CAS  Google Scholar 

  12. Pierson, E. S., Miller, D. D., Callaham, D. A.et al.. Tip-localized calcium entry fluctuates during pollen tube growth,Developmental Biology, 1996, 174(1): 160.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

About this article

Cite this article

Ma, L., Xu, X., Cui, S. et al. Effects of extracellular calmodulin on pollen germination and tube growth. Chin. Sci. Bull. 43, 143–146 (1998). https://doi.org/10.1007/BF02883929

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02883929

Keywords

Navigation