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Expression of tachykinin receptors inXenopus oocytes injected with poly (A)+ RNA from cat dorsal root ganglion

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Abstract

The expression of the types of tachykinin receptors in the dorsal root ganglion (DRG) neurons by means ofXenopus oocyte expressing system was studied. Poly(A)+ RNAs were extracted from cat cervical and lumbar DRG. Two days after injection of Poly (A)+ RNAs, the oocytes were recorded with the two-electrode voltage clamp technique. In the oocytes injected with DRG poly(A)+ RNA, [Sar9, Met(O2)11]-substance P(Sar -SP, 1 μmol/L), neurokinin A (NKA, 1 μmol/L) or [β-Ala8]-neurokinin A(4−10) (Ala-NKA, 1 μmol/L) produced an inward current comprising a rapid spike and a long sustained oscillatory component for several minutes. Sar-SP induced response was blocked by NK-1 antagonist L-668, 169 (1 μmol/L), but not by NK-2 antagonist L-659, 877(1μmol/L). In contrast, Ala-NKA and NKA responses were only blocked by L-659, 877. The oocytes injected with DH Poly(A)+RNA also responded to Sar-SP and NKA with similar inward currents, which were selectively blocked by L-668, 169 and L-659, 877, respectively. These tachykinins-induced responses had a potent desensitization. The present data indicate expression of NK-1 and NK-2 receptors in DRG neurons, suggesting that there may be tachykinin autoreceptors on the nociceptive primary afferent terminals.

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Authors and Affiliations

  1. Shanghai Brain Research Institute, Chinese Academy of Sciences, 200031, Shanghai, China

    Hongsheng Li & Zhiqi Zhao

  2. Shanghai Institute of Physiology, Shinese Academy of Sciences, 200031, Shanghai, China

    Yongde Bao

Authors
  1. Hongsheng Li
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  2. Yongde Bao
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  3. Zhiqi Zhao
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Project supported by the National Natural Science Foundation of China (Grant No. 39370249).

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Li, H., Bao, Y. & Zhao, Z. Expression of tachykinin receptors inXenopus oocytes injected with poly (A)+ RNA from cat dorsal root ganglion. Sci. China Ser. C.-Life Sci. 41, 139–145 (1998). https://doi.org/10.1007/BF02882718

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  • DOI: https://doi.org/10.1007/BF02882718

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