Abstract
Strawberry plants have been regenerated from petioles and flower buds Petiole segments derived from strawberry plants(Fragaria vesca L. andFragaria xananassa Duch. cvs. Gorella and Redgauntlet) maintainedin vitro have been cultured on the Gambobg’s. agar medium supplemented with 2,4-D (2 mg 1−1) either alone or with kinetin or BAP (0.1 mg I−1 or 1 mg I−1). The calli thus obtained have been transferred to media differing from the first ones only by the nature and the concentration of the growth regulators used. One or two months later, shoots appeared on these calli. The influence of the media hormonal content on plant regeneration has been observed. Moreover, genotypic variations have also been investigated: the wild strawberry exhibited the greatest organogenic capacity.
Shoots have also been obtained from flower buds(Fragaria xananassa Duch. cvs. Fern, Hummi Gento, Gorella and Redgauntlet). In this case, shoots appeared directly on small calli derived from the peduncular base of the flower buds. These regenerations have been observed, without changes of media, on the Lee and de Fossabd’s agar medium.
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References
Boxus, P.: The production of strawberry plants byin vitro micro-propagation. - J. hort. Sci.49: 209–210, 1974.
Boxus, P., Quoirin, M., Laine, J. M.: Large scale propagation of strawberry plants from tissue culture. - In: Reinert, J., Bajaj, Y. P. S. (ed.): Applied and Fundamental Aspects of Plant, Cell, Tissue and Organ Culture. Pp. 130–143. Springer Verlag, Berlin -Heidelberg- New York 1977.
De Fossard, R. A., Myint, A., Leb, E. C. M.: A broad spectrum tissue culture experiment with tobacco(Nicotiana tabacum) pith tissue callus. - Physiol. Plant.31: 125–130, 1974.
Flick, C. E.: Isolation of mutants from cells culture. - In: Evans, D.A., Sharp, W. R., Ammirato, P. V., Yamada, Y. (ed.): Handbook of Plant Cell Culture. Volume I. Techniques for Propagation and Breeding. Pp. 393 - 441. Macmillan, Publ., New York 1983.
Flick, C. E., Evans, D. A., Sharp, W. R.: Organogenesis. - In: Evans, D. A., Sharp, W. R., Ammirato, P. V., Yamada, Y. (ed.): Handbook of Plant Cell Culture. Volume I. Techniques for Propagation and Breeding. Pp. 13–81. Macmillan, Publ., New York 1983.
Gambobg, O. L., Miller, R. A., Ojima, K.: Nutrient requirements of suspension cultures of soybean root cells.- Exp. Cell Res.50: 151–158, 1968.
Karp, A., Bright, S. W. J.: On the causes and origins of somaclonal variation. - Oxford Surv. Plant mol. Cell Biol.2: 199–234, 1985.
Laneri, U., Damiano, C: Strawberry anther cultures. - In: Application de la culturein vitro à l’amélioration des plantes potagères. Réunion Eucarpia. Section „Legumes”. INRA, Versailles 1980.
Lee, E.C.M., De Fossard, R. A.: Regeneration of strawberry plants from tissue cultures. - - Proc. int. Plant Propag. Soc.25: 277–285, 1975.
Nishi, S., Ohsawa, K.: Mass production method of virus-free strawberry plants through meristem callus. - Japan agr. Res. Quart.7: 189–194, 1973.
Terzi, M., Sung, Z. R.: Somatic cell genetics of plant. - CRC crit. Rev. Biotechnol.3: 303–330, 1986.
Wang, D., Webgin, W. P., Zimmerman, R. H.: Somatic embryogenesis and plant regeneration from immature embryos of strawberry. - HortScience19: 71–72, 1984.
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Foucault, C., Letouze, R. In vitro: Régénération de plants de fraisier à partir de fragments de pétioles et de bourgeons floraux. Biol Plant 29, 409–414 (1987). https://doi.org/10.1007/BF02882207
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DOI: https://doi.org/10.1007/BF02882207