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Cloning, sequencing and function ofsanA, a gene involved in nikkomycin biosynthesis ofStreptomyces ansochromogenes

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Abstract

Several genetically stable mutants blocked in nikkomycin biosynthesis were obtained after the slightly germinated spores ofStreptomyces ansochromogenes, a nikkomycin producer, were treated with ultra violet radiation. One of the mutants is the same in morpholotical differentiation as the wild type strain and is designated as NBB19. A DNA library was constructed using plasmid pIJ702 as cloning vector, NBB19 as cloning recipient. A 6 kb DNA fragment which can genetically complement NBB19 was cloned when screening the library for antifungal activity. Sequence analysis showed that the 3 kbBgl II -Sal I fragment contains one complete ORF (ORF1) and one partial ORF (ORF2). ORF1 is designated assanA. sanA is 1 365 bp, encoding a protein consisting of 454 amino acid residues. Database searching indicated thatsanA is homologous to the hypothetical methyltransferase inPyrococcus horikoshii with 25% identities and 41% positives. Disruptant ofsanA lost the ability to synthesize nikkomycin. It indicated thatsanA is a novel gene which is essential for nikkomycin biosynthesis.

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Authors and Affiliations

  1. Institute of Microbiology, Chinese Academy of Sciences, 100080, Beijing, China

    Junyong Jia, Wenli Li, Wei Chen, Liping Nie & Huarong Tan

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  1. Junyong Jia
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  2. Wenli Li
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  3. Wei Chen
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  4. Liping Nie
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  5. Huarong Tan
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Correspondence to Huarong Tan.

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Jia, J., Li, W., Chen, W. et al. Cloning, sequencing and function ofsanA, a gene involved in nikkomycin biosynthesis ofStreptomyces ansochromogenes . Sci. China Ser. C.-Life Sci. 43, 30–38 (2000). https://doi.org/10.1007/BF02881715

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  • DOI: https://doi.org/10.1007/BF02881715

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