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Effect of different disintegration techniques and media on yield and appearance of isolated nuclei

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Biologia Plantarum

Abstract

Cell nuclei have been released from various plant tissues (barley leaves, roots and embryos, tobacco leaves and tissue cultures,Vicia faba roots,Arabidopsis thaliana leaves) by several homogenization methods and the optimal method was established for each tissue. The effect of the composition of isolation medium on the yield and appearance of isolated nuclei was also studied. Longer incubation withn-octanol increases the yield considerably in most cases. Low concentrations of osmoticum increase the yield and their adverse effect on the integrity of nuclei is of little significance. Gum arabic has a favourable effect on nuclei isolation from roots only.

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Abbreviations

IM:

isolation medium

References

  • Birnstiel, M. L., Rho, J. H., Chipchase, M. I. H.: Fractionation of isolated pea nuclei. -Biochim. biophys. Acta55: 734–740, 1962.

    CAS  Google Scholar 

  • D’Alessio, G., Trim, A. R.: A method for the isolation of nuclei from leaves. - J. exp. Bot.19: 831–839, 1968.

    Article  Google Scholar 

  • Dick, C.: Isolation of nuclei and histones from roots ofVicia faba. - Arch. Biochem. Biophys.124: 431–435, 1968.

    Article  PubMed  CAS  Google Scholar 

  • Fraseb, D.: Bursting of bacteria by release of gas pressure. - Nature167: 33–34, 1951.

    Article  Google Scholar 

  • Hamilton, R. H., Künsch, U., Temperli, A.: Simple rapid procedures for isolation of tobacco leaf nuclei. - Anal. Biochem.49: 48–57, 1972.

    Article  PubMed  CAS  Google Scholar 

  • Kao, K. N.: A nuclear staining method for plant protoplasts. - In:Gambobg, O. L., Wetteb, L. R. (ed.): Plant Tissue Culture Methods. Pp. 60–61. National Res. Council of Canada, Prairie Regional Laboratory Saskatoon, Saskatchewan, Canada 1975.

    Google Scholar 

  • Kuehl, L.: Isolation of plant nuclei. - Z. Naturforsch.19B: 525–532, 1964.

    Google Scholar 

  • Loewus, M. W., Loewus, E.: The isolation and characterisation of d-glucose-6-phosphate cycloaldolase (NDA-dependent) fromAcer pseudoplatanus L. cell cultures. - Plant Physiol.48: 255–260, 1971.

    PubMed  CAS  Google Scholar 

  • Mennes, A. M., Voogt, E., Libbengy, K. R.: The isolation of nuclei from cultured tobacco pith expiants. - Plant. Sci. Lett.8: 171–177, 1977.

    Article  CAS  Google Scholar 

  • Opatrný, Z.: [Use of plant tissue cultures in genetics.] In Czech. -Thesis, Inst. Exp. Bot., Czechosl. Acad. Sci., Praha 1971.

  • Quail, P. H.: Plant cell fractionation. - Annu. Rev. Plant. Physiol.30: 425–484, 1979.

    Article  CAS  Google Scholar 

  • Short, C. R., Maines, M. D., Davis, L. E.: Preparation of hepatic microsomal fraction for drug metabolism studies by rapid decompression homogenization. - Proc. Soc. exp. Biol. Med.140: 58–65, 1972.

    PubMed  CAS  Google Scholar 

  • Tautydas, K. J.: Mass isolation of pea nuclei. - Plant Physiol.47: 499–503, 1971.

    Article  Google Scholar 

  • Wallach, D. F. H., Soderberg, J., Bricker, L.: The phospholipides of Ehrlich and ascites caroinoma cells composition and intracellular distribution. - Cancer Res.20: 397–402, 1960.

    PubMed  CAS  Google Scholar 

  • Yang, S. E., Street, H. E.: Studies of the growth in culture of plant cells. XXIII. Isolation of nuclei and histones from cultured cells ofAcer pseudoplatanus. - J. exp. Bot.29: 1291–1298, 1978.

    Article  CAS  Google Scholar 

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  1. Institute of Experimental Botany, Czechoslovak Academy of Sciences, Na sádkách 702, 370 05, České Budějovice, Czechoslovakia

    J. Vlasák

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  1. J. Vlasák
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Vlasák, J. Effect of different disintegration techniques and media on yield and appearance of isolated nuclei. Biol Plant 23, 406–413 (1981). https://doi.org/10.1007/BF02880585

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  • DOI: https://doi.org/10.1007/BF02880585

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