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Metabolism of calcium and effect of divalent cations on respiratory activity of yeast mitochondria

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Abstract

The adsorption of Ca2+ to the mitochondria ofSaccharomyces cerevisiae was investigated and it was found that, in contrast with animal mitochondria, Ca2+ is not accumulated through an energydependent process but is more probably adsorbed to mitochondrial membranes. The adsorption magnitude depends both on the amount of added calcium and on the ionic composition of the medium. It was found by study of the effect of divalent cations on the respiratory activity of yeast mitochondria that (a) Ca2+ and Mg2+ inhibit their oxidation competitively with succinate or citrate, the oxidation of NADH not being affected; (b) stimulation of oxidation of NADH and inhibition of oxidation of citrate and succinate may be observed with Ca2+ in the mitochondria ofTorulopsis utilis and with Co2+ in the mitochondria ofSaccharomyces cerevisiae; (c) Zn2+ inhibits the oxidation of NADH and of citrate; (d) the rate of oxidation of NADH in the presence of Cd2+ is several-fold greater than State 3 activity—on the other hand, oxidation of suceinate and citrate is inhibited by cadmium. In comparison with animal mitochondria, the fate of Ca2+ as well as the effects of other divalent cations on the respiratory activity of yeast mitochondria are different.

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  1. Department of Biochemistry, Faculty of Science, Comenius University, Bratislava

    J. Šubík & J. Kolarov

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  1. J. Šubík
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Šubík, J., Kolarov, J. Metabolism of calcium and effect of divalent cations on respiratory activity of yeast mitochondria. Folia Microbiol 15, 448–458 (1970). https://doi.org/10.1007/BF02880189

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