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Laser-induced tobacco protoplast fusion

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Abstract

Laser tweezers can manipulate small particles, such as cells and organelles. When coupling them with laser microbeam selective fusion of two tobacco protoplasts containing some chloroplast was achieved. Physical and biological variables that affect laser trapping and laser-induced fusion were also discussed. The results show that the effect of chloroplast content and distribution on the yield of cell fusion is remarkable.

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Authors and Affiliations

  1. Department of Physics, University of Science and Technology of China, 230026, Hefei, China

    Yinmei Li, Lijie Guan, Liren Lou, Guoqiang Cui, Yuan Yao, Haowei Wang & Chuanshun Cao

  2. Department of Biology, University of Science and Technology of China, 230026, Hefei, China

    Runlong Lu & Xi Chen

Authors
  1. Yinmei Li
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  2. Lijie Guan
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  3. Liren Lou
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  4. Guoqiang Cui
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  5. Yuan Yao
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  6. Haowei Wang
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  7. Chuanshun Cao
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  8. Runlong Lu
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  9. Xi Chen
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Additional information

Project supported by the National Natural Science Foundation of China (Grant No. 39470205), State Science and Technology Commision, and Science and Technology Commision of Anhui Province.

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Li, Y., Guan, L., Lou, L. et al. Laser-induced tobacco protoplast fusion. Sci. China Ser. C.-Life Sci. 42, 122–127 (1999). https://doi.org/10.1007/BF02880046

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  • DOI: https://doi.org/10.1007/BF02880046

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