Abstract
Hormonal factors stimulating starch accumulation were extracted with water from the vascular tissue containing expiants of kale (Brassica oleracea L. var.medullosa cv. Krasa) and fractioned by elution chromatography on a column of polyvinylpyrrolidone. Their activities were compared with those of some known phytohormones and other metabolites using a special bioassay based on protein synthesis-dependent starch accumulation in stem pith expiants free of vascular tissue. The highest relative activities of the hormonal factors compared were as follows: Most active eluate fraction (14.0), cytokinins (8.0), auxins (2.3), ethylene precursor (2.0), ABA (0.8), 24-epi-brassinolide (0.7), GA3 (0.4), water control (1.0).
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Abbreviations
- IAA:
-
indol-3-yl-acetic acid
- 2iP:
-
N6-(Δ2-isoppent.enyl)adeuine
- PVP:
-
polyvinylpyrrolidone
- SA:
-
starch accumulation stimulating activity
- Z:
-
trans-zeatin
- ZR:
-
irans-zea,tinriboside
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Luštinec, J., Conrad, K., Kamínek, M. et al. Hormonal factors stimulating starch accumulation in stem expiants of kale. Biol Plant 27, 281–285 (1985). https://doi.org/10.1007/BF02879862
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DOI: https://doi.org/10.1007/BF02879862