Abstract
To study the structure of microbial communities in the biological hydrogen production reactor and determine the ecological function of hydrogen producing bacteria, anaerobic sludge was obtained from the continuous stirred tank reactor (CSTR) in different periods of time, and the diversity and dynamics of microbial communities were investigated by denaturing gradient gel electrophoresis (DGGE). The results of DGGE demonstrated that an obvious shift of microbial population happened from the beginning of star-up to the 28th day, and the ethanol type fermentation was established. After 28 days the structure of microbial community became stable, and the climax community was formed. Comparative analysis of 16S rDNA sequences from reamplifying and sequencing the prominent bands indicated that the dominant population belonged to low G+C Gram-positive bacteria (Clostridium sp. andEthanologenbacterium sp.), β-proteobacteria (Acidovorax sp.), γ-proteobacteria (Kluyvera sp.), Bacteroides (uncultured bacterium SJA-168), and Spirochaetes (uncultured eubacterium E1-K13), respectively. The hydrogen production rate increased obviously with the increase ofEthanologenbacterium sp.,Clostridium sp. and uncultured Spirochaetes after 21 days, meanwhile the succession of ethanol type fermentation was formed. Throughout the succession the microbial diversity increased however it decreased after 21 days. Some types ofClostridium sp.Acidovorax sp.,Kluyvera sp., and Bacteroides were dominant populations during all periods of time. These special populations were essential for the construction of climax community. Hydrogen production efficiency was dependent on both hydrogen producing bacteria and other populations. It implied that the cometabolism of microbial community played a great role of biohydrogen production in the reactors.
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References
Benemann, J., Hydrogen biotechnology: progress and prospects, Nat. Biotechnol., 1996, 14: 1101–1103.
Cortright, R. D., Davada, R. R., Dumesic, J. A., Hydrogen from catalytic reforming of biomass derived hydrocarbons in liquid water, Nature, 2002, 418: 964–967.
Miyake, M., Schnackenberg, J., Nakamura, C., Asada, Y., Miyake, J., Molecular handling of hydrogenase, In Biohydrogen II (eds. Miyake, J., Matsunaga, T., Pietro, A.S.), Amsterdam: Elsevier Publishers, 2001, 205–219.
Das, D., Verziroglu, T. N., Hydrogen production by biological processes: A survey of literature, Int. J. Hydrogen Energy, 2001, 26: 13–28.
Tanisho, S., Ishiwata, Y., Continuous hydrogen production from Molassess by the bacteriumEnterobacter Aerogenes, Int. J. Hydrogen Energy, 1994, 19(10): 807–812.
Ren, N. Q., Wang, B. Z., Hydrogen gas bio-production technology for organic wastewater treatment, China Environ. Sci., 1994, 14(6): 413–415.
Levin, D. B., Pitt, L., Love, M., Biohydrogen production: Prosergy, 2004, 29: 173–185.
Chang, J. S., Lee, K. S., Lin, P. J., Biohydrogen production with fixed-bed bioreactors, Int. J. Hydrogen Energy, 2002, 27: 1167–1174.
Hallenbech, P. C., Benemann, J. R., Biological hydrogen production: Fundamentals and limiting process, Int. J. Hydrogen Energy, 2000, 27: 1185–1193.
Hawkes, R., Dionsdale, D. L., Hawkes, I., Hussy, F. R., Sustainable fermentative hydrogen production: Challenges for process optimization, Int. J. Hydrogen Energy, 2002, 27: 1339–1347.
Ren, N. Q., Wang, B. Z., Huang, J. C., Ethanol-type fermentation from carbohydrate in high rate acidogenic reactor, Biotechnol. & Bioeng., 1997, 54(5): 428–433.
Lin, M., Ren, N. Q., Wang, A. J., Wang, X. J., Cooperation of mixed culturing bacteria in the hydrongen production by fermentation, Environ. Sci., 2003, 24(2): 54–59.
Amann, R. I., Ludwig, W., Schleifer, K. H., Phylogenetic identification andin situ detection of individual microbial cells without cultivation, Microbiol. Rev., 1995, 59(1): 143–169.
Moter, A., Göbel, U. B., Fluorescence in situ hybridization (FISH) for direct visualization of microorganisms, J. Microbiol. Methods., 2000, 41: 85–112.
Muyzer, G., Waal, E. C., Uitterlinden, A. G., Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA, Appl. Environ. Microbiol., 1993, 59: 695–700.
Ueno, Y., Haruta, S., Ishii, M., Igarashi, Y., Microbial community in anaerobic hydrogen-producing microflora enriched from sludge compost, Appl. Microbiol. Biotechnol., 2001, 57: 555–562.
Miller, D. N., Bryant, J. E., Madsen, E. L., Ghiorse, W. C., Evaluation and optimization of DNA extraction and purification procedures for soil and sediment samples, Appl. Environ. Microbiol., 1999, 65: 4715–4724.
Bassam, B. J., Caetano-Anolles, G., Gresshoff, P. M., Fast and sensitive silver staining of DNA in polyacrylamide gels, Anal. Biochem., 1991, 196 (1): 80–83.
Wang, Xiangjing, Ren, Nanqi, Xiang, Wensheng, Partial characteristics of hydrogen production by fermentative hydrogen-producing bacterial strain B49, High Technol. Letters, 2003, 9(3): 65–70.
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Xing, D., Ren, N., Gong, M. et al. Monitoring of microbial community structure and succession in the biohydrogen production reactor by denaturing gradient gel electrophoresis (DGGE). Sci. China Ser. C.-Life Sci. 48, 155–162 (2005). https://doi.org/10.1007/BF02879668
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DOI: https://doi.org/10.1007/BF02879668