Skip to main content
SpringerLink
Log in

Growth rate, sugar consumption, chlortetracycline production and anhydrotetracycline oxygenase activity inStreptomyces aureofaciens

  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

The relationship between the activity of ATC oxygenase, CTC production and growth rate was investigated in a low-producing strain ofStreptomyces aureofaciens, closely related to the wildtype strain, and in a higher-producing variant. Different growth rates were achieved by using glucose, fructose and sucrose as carbon sources. Activity of the enzyme and CTC yield in both strains were inversely proportional to the rate of sugar utilization but in the higherproducing variant sugar utilization was slower than in the low-producing strain. The expression of ATC oxygenase was less sensitive to “catabolite repression” in the higherproduc ing strain. BT, a stimulator of CTC production, markedly inhibited growth of the higher-producing variant in a medium with slowly utilized sugars (fructose and sucrose) but had little effect on growth of the lowproducing strain. It also increased the level of ATC oxygenase in both strains under all experimental conditions. It could be established that there was no obligatory relationship between the increase of antibiotic synthesis and the increase of enzyme activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

CTC:

chlortetracycline

ATC:

anhydrotetracycline

BT:

benzylthiocyanate

References

  • Běhal V., Cudlín J., Vasěk Z.: Regulation of biosynthesis of secondary metabolites. III. Incorporation of 1-14C-acetic acid into fatty acid and chlortetracycline inStreptomyces aureofaciens. Folia Microbiol.14, 117 (1969).

    Google Scholar 

  • Běhal V., Hošťálek Z., Vanék Z.: Anhydrotetracycline oxygenase activity and biosynthesis of tetracyclines inStreptomyces aureofaciens.Biotechnol. Lett. 1, 177 (1979).

    Article  Google Scholar 

  • Běhal V., Prušáková-Grégrová J., Hošťálek Z.: Effect of inorganic phosphate and benzylthiocyanate on the activity of anhydrotetracycline oxygenase inStreptomyces aureofaciens.Folia Microbiol. 27, 102 (1982).

    Article  Google Scholar 

  • Gallo M., Katz E.: Regulation of secondary metabolite biosynthesis: Catabolite repression of phenoxazinone synthase and actinomycin by glucose.J.Bacteriol. 109, 659 (1972).

    PubMed  CAS  Google Scholar 

  • Hošťálek Z.: Relationship between the carbohydrate metabolism ofStreptomyces aureofaciens and the biosynthesis of chlortetracycline. I. The effect of interrupted aeration, inorganic phosphate, and benzylthiocyanate on chlortetracycline biosynthesis.Folia Microbiol. 9, 78 (1964).

    Article  Google Scholar 

  • Hošťálek Z., Blumauerová M., Vaněk Z.: Tetracycline antibiotics, p. 294 inEconomic Microbiology, Vol. 3, Secondary Products of Metabolism (A.H. Rose, ed.). Academic Press, London 1979.

    Google Scholar 

  • Hošťálek Z.: Catabolite regulation of antibiotic biosynthesis.Folia Microbiol. 25, 445 (1980)

    Article  Google Scholar 

  • Levine J., Garlock E.A., Fischbach H.: The chemical assay of aureomycin.J.Am.Pharm.Assoc. 38, 473 (1949).

    CAS  Google Scholar 

  • Makarevich V.G., Upiter G.D., Slugina M.D., Tarasova S.S., Gravin N.F.: Effect of orthophosphate on growth rate ofActinomyces aureofaciens and tetracycline biosynthesis by it.Antibiotics 20, 295 (1975).

    CAS  Google Scholar 

  • Pogell B.M., Sankaran L., Redshaw P.A., McCann P.: Regulation of antibiotic biosynthesis and differentiation inStreptomycetes, p. 543 inMicrobiology (D. Schlessinger, ed.). American Society for Microbiology, Washington 1976.

    Google Scholar 

  • Satoh A., Ogawa H., Satomura Y.: Regulation of N-acetylkanamycin amidohydrolase in the idiophase in kanamycin fermentation.Agr.Biol.Chem. 40, 191 (1976).

    CAS  Google Scholar 

  • Soltero F., Johnson M.J.: Effect of the carbohydrate nutrition of penicillin production byPenicillium chrysogenum Q-176.Appl.Microbiol. 1, 52 (1953).

    PubMed  CAS  Google Scholar 

  • VanHandel E.: Determination of fructose and fructose yielding carbohydrates with cold anthrone.Anal.Biochem. 19, 193 (1967).

    Article  CAS  Google Scholar 

  • VanHandel E.: Direct microdetermination of sucrose.Anal.Biochem. 22, 280 (1968).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biogenesis of Natural Substances, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20, Prague 4

    V. Erban, J. Novotná, V. Běhal & Z. Hošťálek

Authors
  1. V. Erban
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Novotná
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. Běhal
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Z. Hošťálek
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Erban, V., Novotná, J., Běhal, V. et al. Growth rate, sugar consumption, chlortetracycline production and anhydrotetracycline oxygenase activity inStreptomyces aureofaciens . Folia Microbiol 28, 262–267 (1983). https://doi.org/10.1007/BF02879556

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02879556

Keywords

Search

Navigation