Skip to main content
SpringerLink
Log in

Duration-dependent hepatoprotective effects of propolis extract against carbon tetrachloride-induced acute liver damage in rats

  • Published:
Advances in Therapy Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

Propolis is a natural product produced by bees that was discovered through the study of traditional cures and knowledge of indigenous people throughout the world. It is rich in vitamins A, B, C, and E, and in amino acids, copper, iron, manganese, and zinc. The investigators studied the duration-dependent hepatoprotective effects of propolis extract (200 mg/kg, orally) against carbon tetrachloride (CCI4; 1.5 ml/kg, intraperitoneally)-induced liver damage in rats. Administration of CCI4 caused a sharp elevation in the activity of serum transaminases and serum alkaline phosphatase. A significant depletion in hepatically reduced glutathione was observed with significantly enhanced hepatic lipid peroxidation. After CCI4 administration, glycogen contents and activities of alkaline phosphatase, adenosine tri phosphatase, and succinic dehydrogenase were significantly decreased, whereas total protein contents and activity of acid phosphatase were increased in the liver and kidney. Propolis extract reversed alterations in all parameters when administered within 6, 12, and 24 h of toxicant exposure. Propolis therapy produced duration-dependent protection, with maximal protection achieved at 24 h after CCI4 exposure. It is believed that propolis in its natural form has general pharmacologic value and marked hepatoprotective potential because of its composition of minerals, flavonoids, and phenolic compounds.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bankova V. Chemical diversity of propolis and the problem of standardization.J Ethnopharmacol. 2005;100:114–117.

    Article  PubMed  CAS  Google Scholar 

  2. Park SH, Min TS. Caffeic acid phenethyl ester ameliorates changes in IGFs secretion and gene expression in streptozotocin-induced diabetic rats.Life Sci. 2006;78:1741–1747.

    Article  PubMed  CAS  Google Scholar 

  3. Padmavathi R, Senthilnathan P, Chodon D, Sakthisekaran D. Therapeutic effect of paclitaxel and propolis on lipid peroxidation and antioxidant system in 7,12-dimethyl benz(a)anthraceneinduced breast cancer in female Sprague Dawley rats.Life Sci. 2006;78:2820–2825.

    Article  PubMed  CAS  Google Scholar 

  4. Kujumgiev A, Tsretkova I, Serkedjieva Yu, Bankova V, Christov R, Popov S. Antibacterial, antifungal and antiviral activities of propolis of different geographic origin.J Ethnopharmacol. 1999;64:235–240.

    Article  PubMed  CAS  Google Scholar 

  5. Hu F, Hepburn HR, Li Y, Chen M, Radloff SE, Daya S. Effects of ethanol and water extracts of propolis (bee glue) on acute inflammatory animal models.J Ethnopharmacol. 2005;100:276–283.

    Article  PubMed  Google Scholar 

  6. Morello S, Vellecco V, Alfieri A, Mascolo N, Cicala C. Vasorelaxant effect of the flavonoid galangin on isolated rat thoracic aorta.Life Sci. 2006;78:825–830.

    Article  PubMed  CAS  Google Scholar 

  7. Shimizu K, Ashida H, Matsuura Y, Kanazawa K. Antioxidative bioavailability of artepillin C in Brazilian propolis.Arch Biochem Biophys. 2004;424:181–188.

    Article  PubMed  CAS  Google Scholar 

  8. Orsolic N, Knezevic AH, Sver L, Terzic S, Basic I. Immunomodulatory and antimetastatic action of propolis and related polyphenolic compounds.J Ethnopharmacol. 2004;94:307–315.

    Article  PubMed  CAS  Google Scholar 

  9. Banskota AH, Tezuka Y, Kodata SH. Recent progress in pharmacological research of propolis.Phytother Res. 2001;15:561–571.

    Article  PubMed  CAS  Google Scholar 

  10. Pascual C, Gonzalez R, Torriceila RG. Scavenging action of propolis extract against oxygen radicals.J Ethnopharmacol. 1994;41:9–13.

    Article  PubMed  CAS  Google Scholar 

  11. Moreno MI, Isla MI, Sampietro AR, Vattuone MA. Comparison of the free radical scavenging activity of propolis from several regions of Argentina.J Ethnopharmacol. 2000;71:109–114.

    Article  PubMed  CAS  Google Scholar 

  12. Shukla S, Bhadauria M, Jadon A. Effect of propolis extract on acute carbon tetrachloride induced hepatotoxicity.Indian J Exp Biol. 2004;42:993–997.

    PubMed  Google Scholar 

  13. Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases.Am J Clin Pathol. 1957;28:56–63.

    PubMed  CAS  Google Scholar 

  14. Halk PB, Oster BL, Summerson WH.Practical Physiological Chemistry. 14th ed. New York: McGraw Hill; 1954:1123.

    Google Scholar 

  15. Sharma SK, Krishna Murti CR. Production of lipid peroxides by brain.J Neurochem. 1968;15: 147–149.

    Article  PubMed  CAS  Google Scholar 

  16. Brehe JE, Burch HB. Enzymatic assay for glutathione.Anal Biochem. 1976;74:189–197.

    Article  PubMed  CAS  Google Scholar 

  17. Scifter S, Dayton S, Novic B, Muintwyler E. The estimation of glycogen with anthrone reagent.Arch Biochem. 1950;25:191–200.

    Google Scholar 

  18. Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent.J Biol Chem. 1951;193:265–275.

    PubMed  CAS  Google Scholar 

  19. Seth PK, Tangari KK. Biochemical effects of some newer salicylic acid congeners.J Pharm Pharmacol. 1966;18:831–833.

    PubMed  CAS  Google Scholar 

  20. Slatter EC, Bonner WD. The effect of fluoride on the succinic oxidase system.J Biochem. 1952;82:185–191.

    Google Scholar 

  21. Snedecor GW, Cochran WG.Statistical Methods. 8th ed. Ames, Iowa: Iowa State University Press; 1994:217–236.

    Google Scholar 

  22. Kadhska MB, Gladen BC, Baird DD, et al. Biomarkers of oxidative stress study: are plasma antioxidants markers of CCI4 poisoning?Free Radie Biol Med. 2000;28:838–845.

    Article  Google Scholar 

  23. Rajesh MG, Latha MS. Preliminary evaluation of the antihepatotoxic activity of Kamilari, a polyherbal formulation.J Ethnopharmacol. 2004;91:99–104.

    Article  PubMed  CAS  Google Scholar 

  24. Suja SR, Latha PG, Pushpangandan P, Rajasekharan S. Evaluation of hepatoprotective effects ofHelminthostachys zeylanica (L.) Hook against carbon tetrachloride-induced liver damage in Wistar rats.J Ethnopharmacol. 2004;92:61–66.

    Article  PubMed  CAS  Google Scholar 

  25. Rastogi S, Rana SVS. Influence of parathyroidectomy on liver glycogen in rats treated with carbon tetrachloride.Indian J Exp Biol. 1990;28:794–795.

    PubMed  CAS  Google Scholar 

  26. Abraham P, Wilfred G. Lysosomal enzymes in the pathogenesis of carbon tetrachloride induced injury to the kidney and testis in the rat.Indian J Pharmacol. 2000;32:250–251.

    Google Scholar 

  27. Shukla S, Jadon A, Bhadauria M. Comparative effect ofTerminalia belerica fruit extract and its active principle against carbon tetrachloride induced toxicity in rats.Indian J Pharm Sci. 2005;67:681–686.

    CAS  Google Scholar 

  28. Shukla S, Bhadauria M, Sharma A, Jadon A. Hepatoprotective effect of proprietary herbal formulation (PHF) on experimental liver damage in rats.Toxicol Int. 2005;12:75–81.

    Google Scholar 

  29. Lee TY, Mai LM, Wang GJ, Chiu JH, Lin YL, Lin HC. Protective mechanism ofSalvia miltiorrhiza on carbon tetrachloride-induced hepatotoxicity in rats.J Pharmacol Sci. 2003;91:202–210.

    Article  PubMed  CAS  Google Scholar 

  30. Pinchuk I, Lichtenberg D. The mechanism of action of antioxidants against lipoprotein peroxidation: evaluation based on kinetic experiments.Prog Lipid Res. 2002;41:279–314.

    Article  PubMed  CAS  Google Scholar 

  31. Jeon TI, Hwang SG, Park NG, et al. Antioxidative effect of chitosan on chronic carbon tetrachloride induced hepatic injury in rats.Toxicology. 2003;187:67–73.

    Article  PubMed  CAS  Google Scholar 

  32. Lin CC, Yen MH, Lo TS, Lin JM. Evaluation of hepatoprotective and antioxidant activity ofBoehmeria nivea var. nivea and B.nivea var. tenacissima.J Ethnopharmacol. 1998;60:9–17.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Reproductive Biology and Toxicology Laboratory School of Studies in Zoology, Jiwaji University, Gwalior, India

    Monika Bhadauria, Satendra Kumar Nirala & Sangeeta Shukla

Authors
  1. Monika Bhadauria
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Satendra Kumar Nirala
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sangeeta Shukla
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Monika Bhadauria.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bhadauria, M., Nirala, S.K. & Shukla, S. Duration-dependent hepatoprotective effects of propolis extract against carbon tetrachloride-induced acute liver damage in rats. Adv Therapy 24, 1136–1145 (2007). https://doi.org/10.1007/BF02877719

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02877719

Keywords

Search

Navigation