Abstract
A strain ofArthrobacter simplex was selected as source of single cell protein for its good growth on hydrocarbons and its high protein content. The strain was able to grow on purely synthetic medium with hydrocarbons as sole carbon source. Hexadecane and ammonium sulphate were found as the most suitable C and N sources, respectively. Among the organic supplements yeast extract (0.01 %) showed the best effect. The optimum pH was 7.2–7.6 and the optimum temperature for growth was 32 °C. Penicillin, sodium dodecyl sulphate, EDTA and Tween 80 had either a very little stimulatory effect, no effect or an inhibitory effect.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bennett I.C., Hondermarck J.C., Todd J.R.: How BP makes protein from hydrocarbons.Hydrocarbon Process 48, 104 (1969).
Bergey’s Manual of Determinative Bacteriology, 7th ed. (R.E. Buchanan, N.E. Gibbons, Eds). Williams and Wilkins, Baltimore1974.
Chatterjee M.: Production of amino acids from hydrocarbons by microorganisms with special reference to lysine and valine.Ph.D. Thesis, University of Burdwan (India) 1980.
Foster J.W.: Hydrocarbons as substrates for microorganisms.Anton.van Leeuwenhoek J.Microbiol.Serol. 28, 241 (1962).
Ghosh B.B., Banerjee A.K.: Protein content and amino acid composition of some hydrocarbon utilizing microorganisms.Proc.Seminar on Basic and Applied Aspects of Microbial Research, J.N.U., New Delhi 1979.
Ghosh B.B., Banerjee A.K.: Evaluation of hydrocarbon grownArthrobacter cells as a source of protein.Biol.Bull.India 3, 140 (1981).
Humphrey A.E.: Future of large scale fermentation for production of single cell protein, p. 330 inSingle Cell Protein (R.I. Matales, S.R. Tannenbaum, Eds.) MIT Press, Massachusetts 1968.
Iyenger M.S., Baruah J.N.: Protein from petroleum hydrocarbons.Brit.Chem.Eng. 13, 684 (1968).
Ko P.C., Yu Y.: Production of SCP from hydrocarbons, Taiwan, p. 225 inSingle Cell Protein (R.I. Matales, S.R. Tannenbaum, Eds). MIT Press, Massachusetts 1968.
Kharatyan S.G.: Microbes as food for human.Ann.Rev.Microbiol. 32, 301 (1978).
Klug M.J., Markovetz A.J.: Degradation of hydrocarbons by members of the genusCandida. I. Hydrocarbon assimilation.Appl.Microbiol. 15, 680 (1971).
Keddie R.M.: Actinomycetes and related organisms. Genus IIArthrobacter (Conn and Dimick1947), p. 300 inBergey’s Manual of Determinative Bacteriology, 7th ed. (R.E. Buchanan, N.E. Gibbons, Eds). Williams and Wilkins, Baltimore 1974.
Lukins H.B.: On utilization of hydrocarbons, methyl ketones and hydrogen byMycobacteria.PhD Thesis, University of Texas (USA) 1962.
McNab J.G., Rey L.R.: Potential of single cell protein.Ann.Meet.Am.Assoc.Adv.Sci., Washington (D.C.) 1966.
Senez J.C.: Proteins from hydrocarbons.Proc.Symp. Aix-en Provence and Relavant Guideline of the UN, PAG (N.G. Pontanel, Ed.). Published by organizing committee, Paris 1972.
Shacklady C.A.: Yeast grown on hydrocarbons as a new source of protein.World Rev.Nutrit. Diet. 14, 154 (1972).
Vadalkar K., Srivastava S.P., Nigam J.N., Chanda D., Baruah J.N., Krishna P.V., Iyenger M.S.: Production of single cell protein from processed gas oil and recovery of surface active agents and by-products.J.Ferment.Technol. 49, 529 (1971).
Vogel A.I.:Elementary Practical Chemistry, part 3. Longman, Green and Co., London 1958.
Walker T.: Protein production by unicellular organisms from hydrocarbon substrates, p. 328 in theBiological Efficiency of Protein Production (J.G.W. Jones, Ed.). Cambridge Univ. Press 1973.
Wilkinson J.F.: Hydrocarbons as source of SCP, p. 15 in Symp.Soc.Gen.Microbiol. No. 21.Microbes and Biological Productivity, 1971.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ghosh, B.B., Banerjee, A.K. Production of single cell protein from hydrocarbons byArthrobacter simplex 162. Folia Microbiol 29, 222–227 (1984). https://doi.org/10.1007/BF02877312
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02877312