Abstract
The character of protein synthesis between the 10th and the 50th min of cultivation of recipient cells in a TM2 medium was followed with the aid of14C-proline pulse labelling. Some of the peaks of14C-proline incorporation appear to be related to the frequency of transformation. It is assumed on the basis of chloramphenicol inhibition that different proteins are synthesized by recipient cells to ensure reversible as well as irreversible DNA uptake. Their initiation was localized before the 20th min and at about the 30th min, respectively.
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Tichý, P. De novo protein synthesis during the development of competence inBacillus subtilis. Folia Microbiol 17, 213–220 (1972). https://doi.org/10.1007/BF02875816
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DOI: https://doi.org/10.1007/BF02875816