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The effect of bilirubin on lipid peroxidation and antioxidant enzymes in cumene hydroperoxide-treated erythrocytes

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International Journal of Clinical and Laboratory Research

Abstract

Recently, it has been suggested that bilirubin may act as a potent biological chain-breaking antioxidant. To observe the effects of free bilirubin on antioxidant reactions in cumene hydroperoxide-treated erythrocytes (15 g hemoglobin/dl), we added bilirubin at four different concentrations (0.5, 1, 5, and 10 mg/dl). We measured the thiobarbituric acid-reactive substance and reduced glutathione levels, and some antioxidant enzyme activities, namely superoxide dismutase, catalase, and glucose-6-phosphate dehydrogenase. Thiobarbituric acid-reactive substance and chemiluminescent signals decreased during the incubation. Superoxide dismutase activities also decreased but not as much as in the control group. Glucose-6-phosphate dehydrogenase activities and reduced glutathione levels increased, but catalase activities remained the same as the control group. Our results suggest that bilirubin — in the concentrations we have used — partially prevented the oxidant effects of cumene hydroperoxide.

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References

  1. Halliwell B, Gutteridge MC. The antioxidants of human extracellular fluids. Arch Biochem Biophys 1990; 280: 1.

    Article  PubMed  CAS  Google Scholar 

  2. Stocker R, Yamamato Y, McDonagh AF, Glazer AN Ames BN. Bilirubin is an antioxidant of possible physiological importance. Science 1987; 235: 1043.

    Article  PubMed  CAS  Google Scholar 

  3. Stocker R, Peterhans E. Synergistic interaction between vitamin E and the bile pigments bilirubin and biliverdin. Biochem Biophys Acta 1989; 1002: 238.

    PubMed  CAS  Google Scholar 

  4. Fauchere J-C, Meier-Gibbons FE, Koerner F, Bossi E. Retinopathy of prematurity and bilirubin — no clinical evidence for a beneficial role of bilirubin as a physiological anti-oxidant. Eur J Pediatr 1994; 153: 358.

    Article  PubMed  CAS  Google Scholar 

  5. Wu T-W, Fung KP, Wu J, Yang C-C, Weisel RD. Antioxidation of human low density lipoprotein by unconjugated and conjugated bilirubins. Biochem Pharmacol 1996; 51: 859.

    Article  PubMed  CAS  Google Scholar 

  6. Neutzil J, Stocker R. Free and albumin-bound bilirubin are efficient co-antioxidants forα-tocopherol, inhibiting plasma and low density lipoprotein lipid peroxidation. J Biol Chem 1994; 269: 16712.

    Google Scholar 

  7. Jain SK. Evidence for membrane lipid peroxidation during the in vivo aging of human erythrocytes. Biochim Biophys Acta 1988; 937: 205.

    Article  PubMed  CAS  Google Scholar 

  8. Yalçin AS, Sabuncu N, Emerk K. Cumene-hydroperoxide-induced chemiluminescence in human erythrocytes. Effect of antioxidants and sulphydryl compounds. Int J Biochem 1992; 24: 499.

    Article  PubMed  Google Scholar 

  9. Smith JA, Baker MS, Weidemann MJ. Free radical generation in hydroperoxide-treated erythrocytes monitored continuously by luminol-amplified chemiluminescence. Biochem Int 1992; 28: 1009.

    PubMed  CAS  Google Scholar 

  10. Yeşilkaya A, Yeğin A, Yucel G, Aliciguzel Y, Aksu TA. Continuous monitoring of hydroperoxide-induced peroxidation in human erythrocytes by low-level chemiluminescence. Int J Clin Lab Res 1996; 26: 60.

    Article  PubMed  Google Scholar 

  11. Doğan P, Soyuer Ü, Tanrikulu G. Superoxide dismutase and myeloperoxidase activity in polymorphonuclear leukocytes, and serum ceruloplasmin and copper levels in psoriasis. Br J Dermatol 1989; 120: 239.

    PubMed  Google Scholar 

  12. Cohen G, Dembiec D, Marcus J. Measurement of catalase activity in tissue extracts. Anal Biochem 1970; 34: 30.

    Article  PubMed  CAS  Google Scholar 

  13. Beutler E. Red cell metabolism. A manual of biochemical methods, 2nd edn. New York: Grune and Stratton; 1975: 66–69.

    Google Scholar 

  14. Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med 1963; 61: 882.

    PubMed  CAS  Google Scholar 

  15. Frei B, England L, Ames BN. Ascorbate is an outstanding antioxidant in human blood plasma. Proc Natl Acad Sci USA 1989; 86: 6377.

    Article  PubMed  CAS  Google Scholar 

  16. Burmistrov SO, Dubinina EE, Shabalov NP, Khodov DA, Talabi E. In vitro effect of bilirubin on antioxidant activity in the plasma and erythrocytes of cord blood. Bull Exp Biol Med 1993; 116: 823.

    Article  Google Scholar 

  17. Gumuslu S, Demir AY, Yucel G, Yeşilkaya A, Aydin M, Aksu TA. Antioxidant role of bovine albumin detected by a chemiluminescence method. Turk J Med Sci 1996; 26: 431.

    CAS  Google Scholar 

  18. Pryor WA. The role of free radical reactions in biological systems. In: Pryor W, ed. Free radicals in biology, vol 1. San Diego: Academic Press; 1976: 1–49.

    Google Scholar 

  19. Pigeolet E, Corbisier P, Houbion A, Lambert D, Michiels C, Raes M, Zachary MD, Remacle J. Glutathione peroxidase, superoxide dismutase, and catalase inactivation by peroxides and oxygen derived free radicals. Mech Ageing Dev 1990; 51: 283.

    Article  PubMed  CAS  Google Scholar 

  20. Kuypers FA, Scott MD, Schott MA, Lubin B, Chiu DT. Use of ektacytometry to determine red cell susceptibility to oxidative stress. J Lab Clin Med 1990; 116: 535.

    PubMed  CAS  Google Scholar 

  21. Yeşilkaya A, Yeğin A. Inhibition of human erythrocyte (Na+-K+) ATPase by organic hydroperoxides and protection by ascorbic acid and butylated hydroxtoluene. Gen Pharmacol 1998; 30: 495.

    Article  PubMed  Google Scholar 

  22. Kwak JY, Takeshige K, Cheung BS, Minekami S. Bilirubin inhibits the activation of superoxide-producing NADPH oxidase in a neutrophil cell-free system. Biochem Biophys Acta 1991; 1076: 369.

    PubMed  CAS  Google Scholar 

  23. Chiu D, Kuypers F, Lubin B. Lipid peroxidation in human red cells. Semin Hematol 1989; 26: 257.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. School of Medicine, Department of Biochemistry, Akdeniz University, TR-07070, Antalya, Turkey

    A. Yeşilkaya, A. Yeğin, S. Özdem & T. A. Aksu

Authors
  1. A. Yeşilkaya
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  2. A. Yeğin
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  3. S. Özdem
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  4. T. A. Aksu
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Correspondence to A. Yeşilkaya.

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Yeşilkaya, A., Yeğin, A., Özdem, S. et al. The effect of bilirubin on lipid peroxidation and antioxidant enzymes in cumene hydroperoxide-treated erythrocytes. Int J Clin Lab Res 28, 230–234 (1998). https://doi.org/10.1007/BF02874114

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  • DOI: https://doi.org/10.1007/BF02874114

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